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Literature summary extracted from

  • Troncoso-Ponce, M.A.; Rivoal, J.; Venegas-Caleron, M.; Dorion, S.; Sanchez, R.; Cejudo, F.J.; Garces, R.; Martinez-Force, E.
    Molecular cloning and biochemical characterization of three phosphoglycerate kinase isoforms from developing sunflower (Helianthus annuus L.) seeds (2012), Phytochemistry, 79, 27-38.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.3 expressed in Escherichia coli as a His-tagged fusion protein Helianthus annuus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.3 ADP 0.1 mM, 63.1% activity; 0.1 mM, 85% activity; 0.1 mM, 86% activity; 1 mM, 31% activity; 1 mM, 32% activity Helianthus annuus
2.7.2.3 AMP 0.625 mM, 95% activity; 0.625 mM, 98% activity; 10 mM, 40% activity; 10 mM, 44% activity; 10 mM, 50% activity Helianthus annuus
2.7.2.3 Glycerol 2-phosphate 10 mM, 97% activity Helianthus annuus
2.7.2.3 glycerol 3-phosphate 10 mM, 92% activity; 10 mM, 95% activity; 10 mM, 97% activity Helianthus annuus
2.7.2.3 UDP 10 mM, 94.3% activity; 10 mM, 97% activity Helianthus annuus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.3 0.215
-
 ATP pH 8.2, 25°C Helianthus annuus
2.7.2.3 0.227
-
 ATP pH 7.6, 25°C Helianthus annuus
2.7.2.3 0.243
-
 ATP pH 7, 25°C Helianthus annuus
2.7.2.3 0.3
-
 ATP pH 7, 25°C Helianthus annuus
2.7.2.3 0.356
-
 ATP pH 8.2, 25°C Helianthus annuus
2.7.2.3 0.357
-
 ATP pH 7, 25°C Helianthus annuus
2.7.2.3 0.367
-
 ATP pH 7.6, 25°C Helianthus annuus
2.7.2.3 0.404
-
 ATP pH 8.2, 25°C Helianthus annuus
2.7.2.3 0.406
-
 ATP pH 7.6, 25°C Helianthus annuus
2.7.2.3 0.589
-
 3-phospho-D-glycerate pH 8.2, 25°C Helianthus annuus
2.7.2.3 0.601
-
 3-phospho-D-glycerate pH 7, 25°C Helianthus annuus
2.7.2.3 0.683
-
 3-phospho-D-glycerate pH 8.2, 25°C Helianthus annuus
2.7.2.3 0.707
-
 3-phospho-D-glycerate pH 7, 25°C Helianthus annuus
2.7.2.3 0.711
-
 3-phospho-D-glycerate pH 7.6, 25°C Helianthus annuus
2.7.2.3 0.719
-
 3-phospho-D-glycerate pH 7.6, 25°C Helianthus annuus
2.7.2.3 1.635
-
 3-phospho-D-glycerate pH 8.2, 25°C Helianthus annuus
2.7.2.3 1.787
-
 3-phospho-D-glycerate pH 7.6, 25°C Helianthus annuus
2.7.2.3 1.948
-
 3-phospho-D-glycerate pH 7, 25°C Helianthus annuus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.2.3 cytosol
-
 Helianthus annuus 5829
-
2.7.2.3 plastid
-
 Helianthus annuus 9536
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.3 42300
-
 calculated from cDNA Helianthus annuus
2.7.2.3 42400
-
 calculated from cDNA Helianthus annuus
2.7.2.3 45000
-
 SDS-PAGE, recombinant protein Helianthus annuus

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.3 Helianthus annuus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.3 using Ni-NTA chromatography Helianthus annuus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.2.3 cotyledon
-
 Helianthus annuus
-
2.7.2.3 cotyledon high expression Helianthus annuus
-
2.7.2.3 leaf
-
 Helianthus annuus
-
2.7.2.3 leaf high expression Helianthus annuus
-
2.7.2.3 root
-
 Helianthus annuus
-
2.7.2.3 stem
-
 Helianthus annuus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Helianthus annuus ADP + 3-phospho-D-glyceroyl phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.2.3 HacPGK1
-
 Helianthus annuus
2.7.2.3 HacPGK2
-
 Helianthus annuus
2.7.2.3 HapPGK
-
 Helianthus annuus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.2.3 25
-
 assay at Helianthus annuus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.2.3 65
-
 80% activity at 65°C, then rapidly decreasing between 65-80°C Helianthus annuus
2.7.2.3 65
-
 enzymatic activity is completely lost at temperatures over 65°C Helianthus annuus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.3 7.8
-
 assay at Helianthus annuus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.2.3 7.5 8.75 optimal pH range Helianthus annuus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.2.3 7 9.2 75% activity retained at pH 9.2, a sharp reduction of activity below pH 7 Helianthus annuus
2.7.2.3 9.2
-
 75% activity retained at pH 9.2 Helianthus annuus

Expression

EC Number Organism Comment Expression
2.7.2.3 Helianthus annuus HacPGK1 shows the highest level of expression during seed development, especially at the initial stages, 12 days after flowering (DAF), and accounts for most (approximately 89%) of the combined expression of the three genes. As seed development progresses, the level of expression for this gene decreases up
2.7.2.3 Helianthus annuus highest expression of HapPGK in photosynthetically active tissues, cotyledons and leaves up