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Literature summary extracted from

  • Jones, D.C.; Alphey, M.S.; Wyllie, S.; Fairlamb, A.H.
    Chemical, genetic and structural assessment of pyridoxal kinase as a drug target in the African trypanosome (2012), Mol. Microbiol., 86, 51-64.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.35 expressed in Escherichia coli BL21(DE3) cells Trypanosoma brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.35 4-deoxypyridoxine
-
 Trypanosoma brucei
2.7.1.35 Ca2+ low substrate inhibition Trypanosoma brucei
2.7.1.35 ginkgotoxin i.e.4'-O-methylpyridoxine Trypanosoma brucei
2.7.1.35 Mg2+ low substrate inhibition Trypanosoma brucei
2.7.1.35 roscovitine
-
 Trypanosoma brucei
2.7.1.35 Zn2+ ZnCl2 shows pronounced high substrate inhibition Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.35 0.00165
-
 pyridoxal recombinant enzyme, apparent value, in HEPES/MgCl2 pH 7.4, 0.15 mM ZnCl2, at 37°C Trypanosoma brucei
2.7.1.35 0.038
-
 pyridoxal recombinant enzyme, apparent value, in 70 mM potassium phosphate pH 7.0, 0.15 mM ZnCl2, at 37°C Trypanosoma brucei
2.7.1.35 0.0466
-
 ginkgotoxin recombinant enzyme, apparent value, in HEPES/MgCl2 pH 7.4, 0.15 mM ZnCl2, at 37°C Trypanosoma brucei
2.7.1.35 0.048
-
 pyridoxine recombinant enzyme, apparent value, in HEPES/MgCl2 pH 7.4, 0.15 mM ZnCl2, at 37°C Trypanosoma brucei
2.7.1.35 0.0594
-
 pyridoxamine recombinant enzyme, apparent value, in HEPES/MgCl2 pH 7.4, 0.15 mM ZnCl2, at 37°C Trypanosoma brucei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.35 Co2+
-
 Trypanosoma brucei
2.7.1.35 Cu2+
-
 Trypanosoma brucei
2.7.1.35 Fe2+
-
 Trypanosoma brucei
2.7.1.35 Mg2+ best activator, optimum activity at 0.25 mM Mn2+ Trypanosoma brucei
2.7.1.35 Mn2+ optimum activity at 0.05 mM Mn2+ Trypanosoma brucei
2.7.1.35 additional information ZnCl2 and Ca2+ do not activate the enzyme Trypanosoma brucei
2.7.1.35 Ni2+
-
 Trypanosoma brucei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.35 33000
-
 x * 33000, SDS-PAGE Trypanosoma brucei

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.35 Trypanosoma brucei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.35 Q-Sepharose column chromatography and Superdex 75 gel filtration Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.35 ATP + ginkgotoxin ginkgotoxin is an effective pseudosubstrate Trypanosoma brucei ?
-
 ?
2.7.1.35 ATP + pyridoxal
-
 Trypanosoma brucei ADP + pyridoxal 5'-phosphate
-
 ?
2.7.1.35 ATP + pyridoxamine
-
 Trypanosoma brucei ADP + pyridoxamine 5'-phosphate
-
 ?
2.7.1.35 ATP + pyridoxine
-
 Trypanosoma brucei ADP + pyridoxine 5'-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.35 ? x * 33000, SDS-PAGE Trypanosoma brucei

Synonyms

EC Number Synonyms Comment Organism
2.7.1.35 PdxK
-
 Trypanosoma brucei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.35 106
-
 ginkgotoxin recombinant enzyme, in HEPES/MgCl2 pH 7.4, 0.15 mM ZnCl2, at 37°C Trypanosoma brucei
2.7.1.35 122
-
 pyridoxine recombinant enzyme, in HEPES/MgCl2 pH 7.4, 0.15 mM ZnCl2, at 37°C Trypanosoma brucei
2.7.1.35 127
-
 pyridoxamine recombinant enzyme, in HEPES/MgCl2 pH 7.4, 0.15 mM ZnCl2, at 37°C Trypanosoma brucei
2.7.1.35 131
-
 pyridoxal recombinant enzyme, in HEPES/MgCl2 pH 7.4, 0.15 mM ZnCl2, at 37°C Trypanosoma brucei

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.35 0.0398
-
 Zn2+ recombinant enzyme, apparent value, in 70 mM potassium phosphate pH 7.0, 0.15 mM ZnCl2, at 37°C Trypanosoma brucei
2.7.1.35 1.54
-
 Mg2+ recombinant enzyme, apparent value, in 70 mM potassium phosphate pH 7.0, 0.15 mM ZnCl2, at 37°C Trypanosoma brucei

General Information

EC Number General Information Comment Organism
2.7.1.35 physiological function the enzyme is essential for growth of Trypanosoma brucei in vitro and for infectivity in mice Trypanosoma brucei

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.35 0.000214
-
 pyridoxamine recombinant enzyme, apparent value, in HEPES/MgCl2 pH 7.4, 0.15 mM ZnCl2, at 37°C Trypanosoma brucei
2.7.1.35 0.000228
-
 ginkgotoxin recombinant enzyme, apparent value, in HEPES/MgCl2 pH 7.4, 0.15 mM ZnCl2, at 37°C Trypanosoma brucei
2.7.1.35 0.000254
-
 pyridoxine recombinant enzyme, apparent value, in HEPES/MgCl2 pH 7.4, 0.15 mM ZnCl2, at 37°C Trypanosoma brucei
2.7.1.35 0.0009
-
 pyridoxal recombinant enzyme, apparent value, in HEPES/MgCl2 pH 7.4, 0.15 mM ZnCl2, at 37°C Trypanosoma brucei