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Literature summary extracted from

  • Luque-Almagro, V.M.; Merchan, F.; Blasco, R.; Igeno, M.I.; Martinez-Luque, M.; Moreno-Vivian, C.; Castillo, F.; Roldan, M.D.
    Cyanide degradation by Pseudomonas pseudoalcaligenes CECT5344 involves a malate:quinone oxidoreductase and an associated cyanide-insensitive electron transfer chain (2011), Microbiology, 157, 739-746.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.5.4 membrane
-
 Pseudomonas oleovorans 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.5.4 (S)-malate + ubiquinone Pseudomonas oleovorans
-
 oxaloacetate + ubiquinol
-
 ?
1.1.5.4 (S)-malate + ubiquinone Pseudomonas oleovorans CECT 5344
-
 oxaloacetate + ubiquinol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.4 Pseudomonas oleovorans
-
 isolated from the Guadalquivir River
-
1.1.5.4 Pseudomonas oleovorans CECT 5344
-
 isolated from the Guadalquivir River
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.4 (S)-malate + ubiquinone
-
 Pseudomonas oleovorans oxaloacetate + ubiquinol
-
 ?
1.1.5.4 (S)-malate + ubiquinone with dichlorophenolindophenol as terminal acceptor Pseudomonas oleovorans oxaloacetate + ubiquinol
-
 ?
1.1.5.4 (S)-malate + ubiquinone
-
 Pseudomonas oleovorans CECT 5344 oxaloacetate + ubiquinol
-
 ?
1.1.5.4 (S)-malate + ubiquinone with dichlorophenolindophenol as terminal acceptor Pseudomonas oleovorans CECT 5344 oxaloacetate + ubiquinol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.5.4 malate: quinone oxidoreductase
-
 Pseudomonas oleovorans
1.1.5.4 malate:quinone oxidoreductase
-
 Pseudomonas oleovorans
1.1.5.4 Mqo
-
 Pseudomonas oleovorans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.5.4 30
-
 assay at Pseudomonas oleovorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.4 8
-
 assay at Pseudomonas oleovorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.4 additional information the enzyme is active with 2,6-dichlorophenolindophenol Pseudomonas oleovorans
1.1.5.4 ubiquinone
-
 Pseudomonas oleovorans

General Information

EC Number General Information Comment Organism
1.1.5.4 additional information oxaloacetate reacts chemically inside the cyanide-insensitive cells to produce a cyanohydrin (2-hydroxynitrile), which is further converted to ammonium. The nitrile is transiently accumulated in cyanide-containing media Pseudomonas oleovorans
1.1.5.4 physiological function the enzyme is a component of the electron transfer chain in Pseudomonas pseudoalcaligenes CECT5344 cells, overview Pseudomonas oleovorans