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Literature summary extracted from

  • Koenigsknecht, M.; Fenlon, L.; Downs, D.
    Phosphoribosylpyrophosphate synthetase (PrsA) variants alter cellular pools of ribose 5-phosphate and influence thiamine synthesis in Salmonella enterica (2010), Microbiology, 156, 950-959.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.6.1 expressed in Escherichia coli BL21(AI) cells Salmonella enterica

Protein Variants

EC Number Protein Variants Comment Organism
2.7.6.1 A181D the mutant shows reduced specific activity compared to the wild type enzyme Salmonella enterica
2.7.6.1 D224A the mutant shows reduced specific activity compared to the wild type enzyme Salmonella enterica
2.7.6.1 E306K the mutant shows reduced specific activity compared to the wild type enzyme Salmonella enterica
2.7.6.1 G209V the mutant shows 45fold reduced specific activity compared to the wild type enzyme Salmonella enterica
2.7.6.1 G50D the mutant shows reduced specific activity compared to the wild type enzyme Salmonella enterica

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.1 Salmonella enterica
-
-
-
2.7.6.1 Salmonella enterica DM10373
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.6.1 Ni2+ resin column chromatography Salmonella enterica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.6.1 0.016
-
 mutant enzyme G209V, at pH 7.5 and 37°C Salmonella enterica
2.7.6.1 0.027
-
 mutant enzyme D224A, at pH 7.5 and 37°C Salmonella enterica
2.7.6.1 0.032
-
 mutant enzyme G50D, at pH 7.5 and 37°C Salmonella enterica
2.7.6.1 0.041
-
 mutant enzyme E306K, at pH 7.5 and 37°C Salmonella enterica
2.7.6.1 0.054
-
 mutant enzyme A181D, at pH 7.5 and 37°C Salmonella enterica
2.7.6.1 0.094
-
 wild type enzyme, at pH 7.5 and 37°C Salmonella enterica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate
-
 Salmonella enterica AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ir
2.7.6.1 ATP + D-ribose 5-phosphate
-
 Salmonella enterica DM10373 AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ir

Synonyms

EC Number Synonyms Comment Organism
2.7.6.1 phosphoribosylpyrophosphate synthetase
-
 Salmonella enterica
2.7.6.1 PRPP synthetase
-
 Salmonella enterica
2.7.6.1 PrsA
-
 Salmonella enterica