Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Grundmann, A.; Li, S.M.
    Overproduction, purification and characterization of FtmPT1, a brevianamide F prenyltransferase from Aspergillus fumigatus (2005), Microbiology, 151, 2199-2207.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.106 expression in Escherichia coli Aspergillus fumigatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.106 Mg2+ at 2-5 mM, slight activation. Inhibitory above 10 mM Aspergillus fumigatus
2.5.1.106 Mn2+ inhibitory above 5 mM Aspergillus fumigatus
2.5.1.106 additional information not inhibitory: EDTA up to 5 mM Aspergillus fumigatus
2.5.1.106 Zn2+ inhibitory above 5 mM Aspergillus fumigatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.106 0.055
-
 brevianamide F pH 7.5, 30°C Aspergillus fumigatus
2.5.1.106 0.074
-
 dimethylallyl diphosphate pH 7.5, 30°C Aspergillus fumigatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.106 Ca2+ at 2-5 mM, slight activation Aspergillus fumigatus
2.5.1.106 Mg2+ at 2-5 mM, slight activation. Inhibitory above 10 mM Aspergillus fumigatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.106 49000
-
 1 * 49000, SDS-PAGE, 1 * 53400, calculated Aspergillus fumigatus
2.5.1.106 53400
-
 1 * 49000, SDS-PAGE, 1 * 53400, calculated Aspergillus fumigatus
2.5.1.106 66000
-
 gel filtration Aspergillus fumigatus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.106 Aspergillus fumigatus Q4WAW7
-
-
2.5.1.106 Aspergillus fumigatus Af293 Q4WAW7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.106 dimethylallyl diphosphate + brevianamide F catalyzes the prenylation of cyclo-L-Trp-L-Pro , i.e.brevianamide F, at the C-2 position of the indole nucleus Aspergillus fumigatus diphosphate + tryprostatin B
-
 ?
2.5.1.106 dimethylallyl diphosphate + brevianamide F catalyzes the prenylation of cyclo-L-Trp-L-Pro , i.e.brevianamide F, at the C-2 position of the indole nucleus Aspergillus fumigatus Af293 diphosphate + tryprostatin B
-
 ?
2.5.1.106 dimethylallyl diphosphate + cyclo-(D-Trp-L-Pro) 32.6% of the activity with brevianamide F Aspergillus fumigatus diphosphate + cyclo-(D-2-dimethylallyl-Trp-L-Pro)
-
 ?
2.5.1.106 dimethylallyl diphosphate + cyclo-(D-Trp-L-Pro) 32.6% of the activity with brevianamide F Aspergillus fumigatus Af293 diphosphate + cyclo-(D-2-dimethylallyl-Trp-L-Pro)
-
 ?
2.5.1.106 dimethylallyl diphosphate + cyclo-(L-Trp-Gly) 4.7% of the activity with brevianamide F Aspergillus fumigatus diphosphate + cyclo-(L-2-dimethylallyl-Trp-Gly)
-
 ?
2.5.1.106 dimethylallyl diphosphate + cyclo-(L-Trp-Gly) 4.7% of the activity with brevianamide F Aspergillus fumigatus Af293 diphosphate + cyclo-(L-2-dimethylallyl-Trp-Gly)
-
 ?
2.5.1.106 dimethylallyl diphosphate + cyclo-(L-Trp-L-Leu) 21.9% of the activity with brevianamide F Aspergillus fumigatus diphosphate + cyclo-(D-2-dimethylallyl-Trp-L-Leu)
-
 ?
2.5.1.106 dimethylallyl diphosphate + cyclo-(L-Trp-L-Leu) 21.9% of the activity with brevianamide F Aspergillus fumigatus Af293 diphosphate + cyclo-(D-2-dimethylallyl-Trp-L-Leu)
-
 ?
2.5.1.106 dimethylallyl diphosphate + cyclo-(L-Trp-L-Phe) 3.4% of the activity with brevianamide F Aspergillus fumigatus diphosphate + cyclo-(L-2-dimethylallyl-Trp-L-Phe)
-
 ?
2.5.1.106 dimethylallyl diphosphate + cyclo-(L-Trp-L-Trp) 6.4% of the activity with brevianamide F Aspergillus fumigatus diphosphate + cyclo-(L-2-dimethylallyl-Trp-L-Trp)
-
 ?
2.5.1.106 dimethylallyl diphosphate + cyclo-(L-Trp-L-Tyr) 5.0% of the activity with brevianamide F Aspergillus fumigatus diphosphate + cyclo-(L-2-dimethylallyl-Trp-L-Tyr)
-
 ?
2.5.1.106 additional information enzyme shows a high substrate specificity towards dimethylallyl diphosphate, but accepts different tryptophan-containing cyclic dipeptides. No substrates: isopentenyl diphosphate or geranyl diphosphate Aspergillus fumigatus ?
-
 ?
2.5.1.106 additional information enzyme shows a high substrate specificity towards dimethylallyl diphosphate, but accepts different tryptophan-containing cyclic dipeptides. No substrates: isopentenyl diphosphate or geranyl diphosphate Aspergillus fumigatus Af293 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.106 monomer 1 * 49000, SDS-PAGE, 1 * 53400, calculated Aspergillus fumigatus

Synonyms

EC Number Synonyms Comment Organism
2.5.1.106 FtmPT1
-
 Aspergillus fumigatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.106 5.57
-
 brevianamide F pH 7.5, 30°C Aspergillus fumigatus