Literature summary extracted from

de Vos, W.M.; Voorhorst, W.G.; Dijkgraaf, M.; Kluskens, L.D.; van der Oost, J.; Siezen, R.J.
Purification, characterization, and molecular modeling of pyrolysin and other extracellular thermostable serine proteases from hyperthermophilic microorganisms (2001), Methods Enzymol., 330, 383-393.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.4.21.B55	-	Pyrococcus furiosus

General Stability

EC Number	General Stability	Organism
3.4.21.B55	glycoprotein	Pyrococcus furiosus

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
3.4.21.B55	Ca2+	stimulates	Pyrococcus furiosus

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
3.4.21.B55	80000	-	x * 80000, low-molecular weight form, SDS-PAGE	Pyrococcus furiosus
3.4.21.B55	105000	-	x * 105000, high-molecular weight form, SDS-PAGE	Pyrococcus furiosus

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.4.21.B55	Pyrococcus furiosus	P72186	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.4.21.B55	-	Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.4.21.B55	casein + H2O	-	Pyrococcus furiosus	?	-	?

Subunits

EC Number	Subunits	Comment	Organism
3.4.21.B55	?	x * 105000, high-molecular weight form, SDS-PAGE	Pyrococcus furiosus
3.4.21.B55	?	x * 80000, low-molecular weight form, SDS-PAGE	Pyrococcus furiosus

Temperature Stability [°C]

EC Number	Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
3.4.21.B55	100	-	half-life: 4 h	Pyrococcus furiosus

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Release 2023.1 (January 2023)