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Literature summary extracted from

  • Yoshikane, Y.; Tamura, A.; Yokochi, N.; Ellouze, K.; Yamamura, E.; Mizunaga, H.; Fujimoto, N.; Sakamoto, K.; Sawa, Y.; Yagi, T.
    Engineering Mesorhizobium loti pyridoxamine-pyruvate aminotransferase for production of pyridoxamine with L-glutamate as an amino donor (2010), J. Mol. Catal. B, 67, 104-110.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.30 expressed in Escherichia coli BL21(DE3) Mesorhizobium loti

Protein Variants

EC Number Protein Variants Comment Organism
2.6.1.30 M2T/V70K M2: surface residue Mesorhizobium loti
2.6.1.30 M2T/V70K/E212G E212: surface residue Mesorhizobium loti
2.6.1.30 M2T/Y35H/V70K Y35: active site residur Mesorhizobium loti
2.6.1.30 M2T/Y35H/V70K/E212G E212: surface residue Mesorhizobium loti
2.6.1.30 V70K active site residue, increase in reactivity toward 2-oxoglutarate Mesorhizobium loti
2.6.1.30 V70K/E212G E212: surface residue Mesorhizobium loti
2.6.1.30 V70R active site residue, elimination of activity Mesorhizobium loti
2.6.1.30 V70R/F247C F247: hydrophobic core residue Mesorhizobium loti
2.6.1.30 Y35H/V70K active site residues Mesorhizobium loti
2.6.1.30 Y35H/V70R/F247C F247: hydrophobic core residue Mesorhizobium loti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.30 8.8
-
 2-oxoglutarate Y35H/V70R/F247C mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 12.6
-
 2-oxoglutarate Y35H/V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 17.2
-
 2-oxoglutarate M2T/Y35H/V70K/E212G mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 21.1
-
 2-oxoglutarate M2T/Y35H/V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 29.3
-
 L-glutamate Y35H/V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 42.4
-
 L-glutamate Y35H/V70R/F247C mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 44.2
-
 L-glutamate M2T/Y35H/V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 51
-
 L-glutamate M2T/Y35H/V70K/E212G mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 61.3
-
 2-oxoglutarate V70R/F247C mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 77.3
-
 2-oxoglutarate M2T/V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 80.1
-
 2-oxoglutarate wild type protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 102.6
-
 2-oxoglutarate V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 106.8
-
 2-oxoglutarate M2T/V70K/E212G mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 110.3
-
 2-oxoglutarate V70K/E212G mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 123.4
-
 L-glutamate wild type protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 133.5
-
 L-glutamate V70R/F247C mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 142
-
 L-glutamate V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.30 41000
-
 4 * 41000, SDS-PAGE, gel filtration Mesorhizobium loti
2.6.1.30 154000
-
 gel filtration Mesorhizobium loti

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.30 Mesorhizobium loti Q988B8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.30 hydrophobic interaction chromatography, gel filtration Mesorhizobium loti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.30 pyridoxamine + 2-oxoglutarate
-
 Mesorhizobium loti pyridoxal + L-glutamate
-
 r
2.6.1.30 pyridoxamine + pyruvate
-
 Mesorhizobium loti pyridoxal + L-alanine
-
 r

Subunits

EC Number Subunits Comment Organism
2.6.1.30 homotetramer 4 * 41000, SDS-PAGE, gel filtration Mesorhizobium loti

Synonyms

EC Number Synonyms Comment Organism
2.6.1.30 pyridoxamine-pyruvate aminotransferase pyridoxamine-2-oxoglutarate aminotransferase activity Mesorhizobium loti

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.30 1.5
-
 L-glutamate wild type protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 1.9
-
 2-oxoglutarate wild type protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 2.7
-
 L-glutamate V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 2.9
-
 L-glutamate Y35H/V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 3
-
 L-glutamate M2T/Y35H/V70K/E212G mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 3.1
-
 L-glutamate M2T/Y35H/V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 3.5
-
 2-oxoglutarate M2T/Y35H/V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 3.9
-
 2-oxoglutarate M2T/V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 4
-
 2-oxoglutarate M2T/Y35H/V70K/E212G mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 4.1
-
 2-oxoglutarate V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 4.4
-
 L-glutamate V70R/F247C mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 4.6
-
 2-oxoglutarate M2T/V70K/E212G mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 4.6
-
 2-oxoglutarate V70K/E212G mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 5.2
-
 2-oxoglutarate Y35H/V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 6.4
-
 L-glutamate Y35H/V70R/F247C mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 8.1
-
 2-oxoglutarate Y35H/V70R/F247C mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 9.1
-
 2-oxoglutarate V70R/F247C mutant protein, pH 9.0, 30°C Mesorhizobium loti

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.6.1.30 0.012
-
 L-glutamate wild type protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 0.019
-
 L-glutamate V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 0.023
-
 2-oxoglutarate wild type protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 0.033
-
 L-glutamate V70R/F247C mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 0.04
-
 2-oxoglutarate V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 0.042
-
 2-oxoglutarate V70K/E212G mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 0.043
-
 2-oxoglutarate M2T/V70K/E212G mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 0.05
-
 2-oxoglutarate M2T/V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 0.058
-
 L-glutamate M2T/Y35H/V70K/E212G mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 0.07
-
 L-glutamate M2T/Y35H/V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 0.1
-
 L-glutamate Y35H/V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 0.148
-
 2-oxoglutarate V70R/F247C mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 0.152
-
 L-glutamate Y35H/V70R/F247C mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 0.167
-
 2-oxoglutarate M2T/Y35H/V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 0.234
-
 2-oxoglutarate M2T/Y35H/V70K/E212G mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 0.408
-
 2-oxoglutarate Y35H/V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.6.1.30 0.916
-
 2-oxoglutarate Y35H/V70R/F247C mutant protein, pH 9.0, 30°C Mesorhizobium loti