EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.3.3 | isoform AK2 fused to maltose-binding protein is expressed in Escherichia coli TB1 cells | Sabellastarte spectabilis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.3.3 | G54A | the mutant shows considerably increased catalytic efficiency for L-arginine and D-arginine compared to the wild type enzyme | Sabellastarte spectabilis |
2.7.3.3 | G54I | the mutant displays undetectable enzymatic activity | Sabellastarte spectabilis |
2.7.3.3 | G54L | the mutant displays undetectable enzymatic activity | Sabellastarte spectabilis |
2.7.3.3 | G54S | the mutant shows increased catalytic efficiency for L-arginine and D-arginine compared to the wild type enzyme | Sabellastarte spectabilis |
2.7.3.3 | G54V | the mutant displays undetectable enzymatic activity | Sabellastarte spectabilis |
2.7.3.3 | L64A | the mutant displays undetectable enzymatic activity | Sabellastarte spectabilis |
2.7.3.3 | L64G | the mutant displays undetectable enzymatic activity | Sabellastarte spectabilis |
2.7.3.3 | L64I | in the mutant, the affinity for L-arginine is greatly increased (9.5fold that of the wild type), whereas its affinity for D-arginine is increased 2.9fold | Sabellastarte spectabilis |
2.7.3.3 | L64V | the mutant shows a 1.7fold decrease in affinity for L-arginine, but unchanged affinity for D-arginine | Sabellastarte spectabilis |
2.7.3.3 | N320A | the mutant shows considerably reduced enzymatic activity (17.1% for L-arginine and 5.19% for D-arginine compared to the wild type enzyme) | Sabellastarte spectabilis |
2.7.3.3 | N320D | the mutant shows considerably reduced enzymatic activity (32.4% for L-arginine and 48.4% for D-arginine compared to the wild type enzyme) | Sabellastarte spectabilis |
2.7.3.3 | N320E | the mutant shows considerably reduced enzymatic activity (52.3% for L-arginine and 10.1% for D-arginine compared to the wild type enzyme) | Sabellastarte spectabilis |
2.7.3.3 | N320H | the mutant shows considerably reduced enzymatic activity (29.5% for L-arginine and 10.2% for D-arginine compared to the wild type enzyme) | Sabellastarte spectabilis |
2.7.3.3 | N320K | the mutant shows considerably reduced enzymatic activity (0.612% for L-arginine and 0.489% for D-arginine compared to the wild type enzyme) | Sabellastarte spectabilis |
2.7.3.3 | N320Q | the mutant shows considerably reduced enzymatic activity (8.97% for L-arginine and 2,6% for D-arginine compared to the wild type enzyme) | Sabellastarte spectabilis |
2.7.3.3 | N320R | the mutant shows considerably reduced enzymatic activity (4.11% for L-arginine and 1.46% for D-arginine compared to the wild type enzyme) | Sabellastarte spectabilis |
2.7.3.3 | Y89Q | the mutant shows increased catalytic efficiency for L-arginine and D-arginine compared to the wild type enzyme | Sabellastarte spectabilis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.3.3 | 0.389 | - |
L-Arg | isoform AK2 mutant L64I, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 0.4 | - |
ATP | isoform AK2 mutant L64I, using L-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 0.661 | - |
ATP | isoform AK2 mutant G54A, using L-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 0.774 | - |
ATP | isoform AK2 mutant L64V, using L-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 0.837 | - |
ATP | isoform AK2 mutant L64I, using D-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 0.926 | - |
ATP | isoform AK2 mutant G54S, using L-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 0.93 | - |
ATP | isoform AK2 mutant Y89Q, using L-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 1.12 | - |
ATP | isoform AK2 mutant G54A, using D-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 1.13 | - |
ATP | wild type enzyme isoform AK2, using L-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 1.59 | - |
ATP | isoform AK2 mutant Y89Q, using D-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 2.05 | - |
L-Arg | isoform AK2 mutant G54S, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 2.72 | - |
L-Arg | isoform AK2 mutant G54A, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 2.99 | - |
ATP | wild type enzyme isoform AK2, using D-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 3.14 | - |
D-Arg | isoform AK2 mutant L64I, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 3.49 | - |
ATP | isoform AK2 mutant G54S, using D-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 3.69 | - |
L-Arg | wild type enzyme isoform AK2, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 3.72 | - |
ATP | isoform AK2 mutant L64V, using D-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 4.4 | - |
D-Arg | isoform AK2 mutant G54A, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 5.07 | - |
L-Arg | isoform AK2 mutant Y89Q, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 6.45 | - |
L-Arg | isoform AK2 mutant L64V, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 6.45 | - |
D-Arg | isoform AK2 mutant Y89Q, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 9 | - |
D-Arg | wild type enzyme, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 9.34 | - |
D-Arg | isoform AK2 mutant G54S, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 9.55 | - |
D-Arg | isoform AK2 mutant L64V, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.3.3 | Sabellastarte spectabilis | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.3.3 | ATP + D-Arg | isoform AK1 shows 7.6% activity with D-arginine compared to L-arginine, isoform AK2 shows 35% activity with D-arginine compared to L-arginine | Sabellastarte spectabilis | ADP + Nomega-phospho-D-Arg | - |
? | |
2.7.3.3 | ATP + L-Arg | isoforms AK1 and AK2 are primarily active towards L-arginine | Sabellastarte spectabilis | ADP + Nomega-phospho-L-Arg | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.3.3 | AK1 | isoform | Sabellastarte spectabilis |
2.7.3.3 | AK2 | isoform | Sabellastarte spectabilis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.3.3 | 33.4 | - |
ATP | isoform AK2 mutant Y89Q, using L-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 36.6 | - |
ATP | wild type enzyme isoform AK2, using D-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 39.5 | - |
ATP | wild type isoform AK2, using L-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 40.8 | - |
D-Arg | wild type enzyme isoform AK2, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 41 | - |
ATP | isoform AK2 mutant G54A, using L-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 41.9 | - |
ATP | isoform AK2 mutant Y89Q, using D-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 43.2 | - |
ATP | isoform AK2 mutant G54S, using L-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 43.3 | - |
ATP | isoform AK2 mutant L64V, using L-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 46 | - |
L-Arg | wild type enzyme isoform AK2, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 50.1 | - |
ATP | isoform AK2 mutant L64I, using L-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 60.7 | - |
ATP | isoform AK2 mutant G54S, using D-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 61.5 | - |
D-Arg | isoform AK2 mutant Y89Q, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 61.8 | - |
ATP | isoform AK2 mutant L64V, using D-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 62.3 | - |
L-Arg | isoform AK2 mutant L64V, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 65.8 | - |
ATP | isoform AK2 mutant G54A, using D-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 68.3 | - |
ATP | isoform AK2 mutant L64I, using D-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 75.4 | - |
L-Arg | isoform AK2 mutant L64I, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 77.8 | - |
L-Arg | isoform AK2 mutant Y89Q, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 81.2 | - |
L-Arg | isoform AK2 mutant G54S, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 86.7 | - |
D-Arg | isoform AK2 mutant G54S, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 90.7 | - |
D-Arg | isoform AK2 mutant L64I, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 90.7 | - |
D-Arg | isoform AK2 mutant L64V, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 121 | - |
L-Arg | isoform AK2 mutant G54A, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 121 | - |
D-Arg | isoform AK2 mutant G54A, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.3.3 | 4.52 | - |
D-Arg | wild type enzyme, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 9.29 | - |
D-Arg | isoform AK2 mutant G54S, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 9.52 | - |
D-Arg | isoform AK2 mutant L64V, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 9.54 | - |
D-Arg | isoform AK2 mutant Y89Q, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 9.65 | - |
L-Arg | isoform AK2 mutant L64V, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 12.5 | - |
L-Arg | wild type enzyme isoform AK2, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 15.4 | - |
L-Arg | isoform AK2 mutant Y89Q, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 27.5 | - |
D-Arg | isoform AK2 mutant G54A, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 29 | - |
D-Arg | isoform AK2 mutant L64I, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 39.5 | - |
L-Arg | isoform AK2 mutant G54S, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 44.7 | - |
L-Arg | isoform AK2 mutant G54A, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 195 | - |
L-Arg | isoform AK2 mutant L64I, in 100 mM Tris/HCl (pH 8.0), at 25°C | Sabellastarte spectabilis |