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Literature summary extracted from

  • Cherney, M.M.; Cherney, L.T.; Garen, C.R.; James, M.N.
    The structures of Thermoplasma volcanium phosphoribosyl pyrophosphate synthetase bound to ribose-5-phosphate and ATP analogs (2011), J. Mol. Biol., 413, 844-856.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.6.1 expressed in Escherichia coli BL21(DE3) cells Thermoplasma volcanium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.6.1 in complex with its substrate D-ribose 5-phosphate and with substrate analogs beta,gamma-methylene ATP and ADP, hanging drop vapor diffusion method, using 2 M ammonium sulfate, pH 4.0 or 30% (w/v) polyethylene glycol 600, pH 7.5 Thermoplasma volcanium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.6.1 Mg2+ required Thermoplasma volcanium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.6.1 55000
-
 gel filtration Thermoplasma volcanium

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.1 Thermoplasma volcanium Q97CA5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate
-
 Thermoplasma volcanium AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ir

Synonyms

EC Number Synonyms Comment Organism
2.7.6.1 phosphoribosyl pyrophosphate synthetase
-
 Thermoplasma volcanium
2.7.6.1 PRPP synthetase
-
 Thermoplasma volcanium