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Literature summary extracted from

  • El-Sayed, A.S.; Khalaf, S.A.; Aziz, H.A.
    Characterization of homocysteine gamma-lyase from submerged and solid cultures of Aspergillus fumigatus ASH (JX006238) (2013), J. Microbiol. Biotechnol., 23, 499-510.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.4.1.2 pyridoxal 5'-phosphate
-
 Aspergillus fumigatus

Application

EC Number Application Comment Organism
4.4.1.2 medicine the enzyme displays a strong affinity to homocysteine, followed by methionine and cysteine when compared with non-S amino acids, confirming its potency against homocysteinuria related diseases, and as an anti-cardiovascular agent and a specific biosensor for homocysteinuria Aspergillus fumigatus

Protein Variants

EC Number Protein Variants Comment Organism
4.4.1.2 up induction of the enzyme is mainly sulfur amino-acid-dependent, especially methionine and homocysteine. The induction of the enzyme in response to various amino acids is evaluated, and the results reveal that sulfur-containing amino acids strongly induce Hcy gamma-lyase production by the isolate when compared with other amino acids. The maximum enzyme productivity is detected in the presence of methionine (1.8 U/mg), homocysteine (1.2 U/mg), cysteine (1.23 U/mg), and asparagine (1.2 U/mg). Some enzyme activity is also detected when using various non-sulfur amino acids as substrates, such as glutamine, alanine, and phenylalanine, indicating the multifunctional deaminating catalytic activity of the crude enzyme. The lowest enzyme activity is detected in the presence of tyrosine and lysine Aspergillus fumigatus
4.4.1.2 up the influence of different carbon sources (0.05-0.2%) on the enzyme is assessed when using 100 mM L-methionine as the substrate. From the data, the highest enzyme yield is obtained with 0.15% sucrose (2.54 U/mg), followed by glucose (2.18 U/mg), representing about 1.42- and 1.2-fold increments, respectively, over the control (1.8 U/mg). The enzyme productivity increases about 12.7fold when using 0.15% sucrose over a carbon-free medium Aspergillus fumigatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.2 dithiothreitol 10 mM, 86% loss of activity Aspergillus fumigatus
4.4.1.2 Hg2+ 10 mM, 95% loss of activity Aspergillus fumigatus
4.4.1.2 hydroxylamine 10 mM, 89% loss of activity Aspergillus fumigatus
4.4.1.2 KCl 10 mM, 11% loss of activity Aspergillus fumigatus
4.4.1.2 additional information no inhibition by EDTA Aspergillus fumigatus
4.4.1.2 SDS 10 mM, 78% loss of activity Aspergillus fumigatus
4.4.1.2 Tween 80 1 mM, about 40% loss of activity Aspergillus fumigatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.2 2.46
-
 L-homocysteine pH 7.0, 35°C Aspergillus fumigatus
4.4.1.2 4.1
-
 L-methionine pH 7.0, 35°C Aspergillus fumigatus
4.4.1.2 4.9
-
 L-cysteine pH 7.0, 35°C Aspergillus fumigatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.4.1.2 extracellular
-
 Aspergillus fumigatus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.2 50000
-
 x * 50000, SDS-PAGE Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.2 L-homocysteine + H2O Aspergillus fumigatus
-
 hydrogen sulfide + NH3 + 2-oxobutanoate
-
 ?
4.4.1.2 L-homocysteine + H2O Aspergillus fumigatus ASH JX006238
-
 hydrogen sulfide + NH3 + 2-oxobutanoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.2 Aspergillus fumigatus
-
-
-
4.4.1.2 Aspergillus fumigatus ASH JX006238
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.2
-
 Aspergillus fumigatus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.2 8.48
-
 pH 7.0, 35°C, solid state fermentation, purified enzyme Aspergillus fumigatus
4.4.1.2 16.6
-
 pH 7.0, 35°C, submerged fermentation, purified enzyme Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.2 glycine + H2O 26.3% of the oxo-acid release activity compared to L-homocysteine as substrate Aspergillus fumigatus ?
-
 ?
4.4.1.2 glycine + H2O 26.3% of the oxo-acid release activity compared to L-homocysteine as substrate Aspergillus fumigatus ASH JX006238 ?
-
 ?
4.4.1.2 L-alanine + H2O 32.1% of the oxo-acid release activity compared to L-homocysteine as substrate Aspergillus fumigatus ?
-
 ?
4.4.1.2 L-alanine + H2O 32.1% of the oxo-acid release activity compared to L-homocysteine as substrate Aspergillus fumigatus ASH JX006238 ?
-
 ?
4.4.1.2 L-asparagine + H2O 33.3% of the oxo-acid release activity compared to L-homocysteine as substrate Aspergillus fumigatus ?
-
 ?
4.4.1.2 L-asparagine + H2O 33.3% of the oxo-acid release activity compared to L-homocysteine as substrate Aspergillus fumigatus ASH JX006238 ?
-
 ?
4.4.1.2 L-cysteine + H2O 43.3% of the oxo-acid release activity compared to L-homocysteine as substrate Aspergillus fumigatus 2-oxopropanoate + NH3 + ?
-
 ?
4.4.1.2 L-cystine + H2O 16.2% of the oxo-acid release activity compared to L-homocysteine as substrate Aspergillus fumigatus ?
-
 ?
4.4.1.2 L-cystine + H2O 16.2% of the oxo-acid release activity compared to L-homocysteine as substrate Aspergillus fumigatus ASH JX006238 ?
-
 ?
4.4.1.2 L-homocysteine + H2O
-
 Aspergillus fumigatus hydrogen sulfide + NH3 + 2-oxobutanoate
-
 ?
4.4.1.2 L-homocysteine + H2O
-
 Aspergillus fumigatus ASH JX006238 hydrogen sulfide + NH3 + 2-oxobutanoate
-
 ?
4.4.1.2 L-lysine + H2O 27.1% of the oxo-acid release activity compared to L-homocysteine as substrate Aspergillus fumigatus ?
-
 ?
4.4.1.2 L-methionine + H2O 90% of the oxo-acid release compared to L-homocysteine as substrate Aspergillus fumigatus 2-oxobutanoate + NH3 + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.2 ? x * 50000, SDS-PAGE Aspergillus fumigatus

Synonyms

EC Number Synonyms Comment Organism
4.4.1.2 Hyc gamma-lyase
-
 Aspergillus fumigatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.4.1.2 37 40
-
 Aspergillus fumigatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.4.1.2 30
-
 T1/2: 18.1 h, at 30°C Aspergillus fumigatus
4.4.1.2 35
-
 T1/2: 9.9 h, at 30°C Aspergillus fumigatus
4.4.1.2 40
-
 stable below, T1/2: 5.9 h, at 30°C Aspergillus fumigatus
4.4.1.2 50
-
 T1/2: 3.3 h, at 30°C Aspergillus fumigatus
4.4.1.2 60
-
 T1/2: 1.9 h, at 30°C Aspergillus fumigatus
4.4.1.2 70
-
 Tm-value is 70.1°C. Retention of about 50% of its initial activity when heated for 60 min at 70.1°C Aspergillus fumigatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4.1.2 0.00077
-
 L-cysteine pH 7.0, 35°C Aspergillus fumigatus
4.4.1.2 0.00097
-
 L-methionine pH 7.0, 35°C Aspergillus fumigatus
4.4.1.2 0.00139
-
 L-homocysteine pH 7.0, 35°C Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.2 7.8
-
-
 Aspergillus fumigatus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.4.1.2 3
-
 4°C, 2 h, 50% reduction in enzyme activity Aspergillus fumigatus
4.4.1.2 6.5 7.8 maximal structural and catalytic stability within the pH-range 6.5-7.8 Aspergillus fumigatus
4.4.1.2 10.4
-
 4°C, 2 h, 20% reduction in enzyme activity Aspergillus fumigatus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.4.1.2 Aspergillus fumigatus isoelectric focusing, detected by pH precipitation with no glycosyl residues
-
 5