BRENDA - Enzyme Database

Phosphatidylserine synthase 2: high efficiency for synthesizing phosphatidylserine containing docosahexaenoic acid

Kimura, A.K.; Kim, H.Y.; J. Lipid Res. 54, 214-222 (2013)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.8.29
expressed in HEK-293 cells; gene PTDSS2, functional recombinant overexpression of FLAG-tagged PSS2 isozyme in HEK-293 and CHO-K1 cells
Mus musculus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.29
endoplasmic reticulum
;
Mus musculus
5783
-
2.7.8.29
membrane
; integral membrane protein; integral membrane protein
Mus musculus
16020
-
2.7.8.29
microsome
;
Mus musculus
-
-
2.7.8.29
mitochondrial membrane
;
Mus musculus
31966
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.8.29
50000
-
; x * 50000, calculated from amino acid sequence
Mus musculus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.8.29
L-1-phosphatidylcholine + L-serine
Mus musculus
isozyme PSS1
L-1-phosphatidylserine + choline
-
-
r
2.7.8.29
L-1-phosphatidylethanolamine + L-serine
Mus musculus
isozyme PSS2
L-1-phosphatidylserine + ethanolamine
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.8.29
Mus musculus
-
-
-
2.7.8.29
Mus musculus
Q99LH2
-
-
2.7.8.29
Mus musculus
Q9Z1X2
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.8.29
anti-FLAG M2 affinity gel chromatography; purification of functional recombinant FLAG-tagged PSS2 from HEK-293 and CHO-K1 cell microsomes by ultracentrifugation, immunoaffinity chromatography
Mus musculus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.29
brain
;
Mus musculus
-
2.7.8.29
additional information
isozyme PSS1 is ubiquitously expressed; isozyme PSS2 is expressed in a tissue-specific manner. It is highly expressed in tissues such as brain and testis, where docosahexaenoic acid (DHA, 22:6n-3) is also highly enriched
Mus musculus
-
2.7.8.29
Purkinje neuron
-
Mus musculus
-
2.7.8.29
Sertoli cell
-
Mus musculus
-
2.7.8.29
testis
;
Mus musculus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.29
1,2-diacylphosphatidylethanolamine + L-serine
the substrate is 6times better utilized than phosphatidylethanolamine plasmalogen
722906
Mus musculus
?
-
-
-
?
2.7.8.29
L-1-phosphatidylcholine + L-serine
isozyme PSS1
722906
Mus musculus
L-1-phosphatidylserine + choline
-
-
-
r
2.7.8.29
L-1-phosphatidylethanolamine + L-serine
-
722906
Mus musculus
L-1-phosphatidylserine + ethanolamine
-
-
-
?
2.7.8.29
L-1-phosphatidylethanolamine + L-serine
isozyme PSS2
722906
Mus musculus
L-1-phosphatidylserine + ethanolamine
-
-
-
r
2.7.8.29
additional information
the enzyme synthesizes docosahexaenoic acid-phosphatidylserine much more efficiently than phosphatidylserine containing either 18:1 or 20:4 at the sn-2 position. Although no sn-1 fatty acyl preference is noted, phosphatidylserine synthase 2 exhibits significant preference toward docosahexaenoic acid compared with 18:1 or 20:4 at the sn-2 position
722906
Mus musculus
?
-
-
-
-
2.7.8.29
additional information
isozyme PSS1 shows a 2fold higher preference for sn-1 18:0 compared with 16:0 when the sn-2 position contains 18:1
722906
Mus musculus
?
-
-
-
-
2.7.8.29
additional information
the recombinant FLAG-tagged enzyme is also active with phosphatidylethanolamine plasmalogen, [C18(plasm),22:6-PS], which is six times less effective as a substrate than the corresponding ester-linked phosphatidylethanolamine species, the isozyme is active with oleic acid (OA; 18:1n-9), DHA (DHA; 22:6n-3), or arachidonic acid esters. Compounds analysis by mass spectroscopy. A carbonyl group next to the glycerol backbone in the substrate-binding pocket substantially increases the catalytic activity of PSS2. Isozyme PSS2 shows a 10fold preference for sn-1 18:0, when 22:6 is esterified at the sn-2 position. Synthesis of 18:0,22:6-PS is most efficient compared with 18:0,18:1-PS and with 18:0,20:4-PS. Substrate specificity and acyl chain preference of isozyme PS2, overview
722906
Mus musculus
?
-
-
-
-
2.7.8.29
phosphatidylethanolamine plasmalogen + L-serine
-
722906
Mus musculus
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.8.29
?
x * 50000, about, recombinant FLAG-tagged PSS2, SDS-PAGE; x * 50000, calculated from amino acid sequence
Mus musculus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.8.29
37
-
assay at
Mus musculus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.8.29
7.4
-
assay at
Mus musculus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.8.29
expressed in HEK-293 cells
Mus musculus
2.7.8.29
gene PTDSS2, functional recombinant overexpression of FLAG-tagged PSS2 isozyme in HEK-293 and CHO-K1 cells
Mus musculus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.29
endoplasmic reticulum
-
Mus musculus
5783
-
2.7.8.29
membrane
-
Mus musculus
16020
-
2.7.8.29
membrane
integral membrane protein
Mus musculus
16020
-
2.7.8.29
microsome
-
Mus musculus
-
-
2.7.8.29
mitochondrial membrane
-
Mus musculus
31966
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.8.29
50000
-
x * 50000, calculated from amino acid sequence
Mus musculus
2.7.8.29
50000
-
-
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.8.29
L-1-phosphatidylcholine + L-serine
Mus musculus
isozyme PSS1
L-1-phosphatidylserine + choline
-
-
r
2.7.8.29
L-1-phosphatidylethanolamine + L-serine
Mus musculus
isozyme PSS2
L-1-phosphatidylserine + ethanolamine
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.8.29
anti-FLAG M2 affinity gel chromatography
Mus musculus
2.7.8.29
purification of functional recombinant FLAG-tagged PSS2 from HEK-293 and CHO-K1 cell microsomes by ultracentrifugation, immunoaffinity chromatography
Mus musculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.29
brain
-
Mus musculus
-
2.7.8.29
additional information
isozyme PSS1 is ubiquitously expressed
Mus musculus
-
2.7.8.29
additional information
isozyme PSS2 is expressed in a tissue-specific manner. It is highly expressed in tissues such as brain and testis, where docosahexaenoic acid (DHA, 22:6n-3) is also highly enriched
Mus musculus
-
2.7.8.29
Purkinje neuron
-
Mus musculus
-
2.7.8.29
Sertoli cell
-
Mus musculus
-
2.7.8.29
testis
-
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.29
1,2-diacylphosphatidylethanolamine + L-serine
the substrate is 6times better utilized than phosphatidylethanolamine plasmalogen
722906
Mus musculus
?
-
-
-
?
2.7.8.29
L-1-phosphatidylcholine + L-serine
isozyme PSS1
722906
Mus musculus
L-1-phosphatidylserine + choline
-
-
-
r
2.7.8.29
L-1-phosphatidylethanolamine + L-serine
-
722906
Mus musculus
L-1-phosphatidylserine + ethanolamine
-
-
-
?
2.7.8.29
L-1-phosphatidylethanolamine + L-serine
isozyme PSS2
722906
Mus musculus
L-1-phosphatidylserine + ethanolamine
-
-
-
r
2.7.8.29
additional information
the enzyme synthesizes docosahexaenoic acid-phosphatidylserine much more efficiently than phosphatidylserine containing either 18:1 or 20:4 at the sn-2 position. Although no sn-1 fatty acyl preference is noted, phosphatidylserine synthase 2 exhibits significant preference toward docosahexaenoic acid compared with 18:1 or 20:4 at the sn-2 position
722906
Mus musculus
?
-
-
-
-
2.7.8.29
additional information
isozyme PSS1 shows a 2fold higher preference for sn-1 18:0 compared with 16:0 when the sn-2 position contains 18:1
722906
Mus musculus
?
-
-
-
-
2.7.8.29
additional information
the recombinant FLAG-tagged enzyme is also active with phosphatidylethanolamine plasmalogen, [C18(plasm),22:6-PS], which is six times less effective as a substrate than the corresponding ester-linked phosphatidylethanolamine species, the isozyme is active with oleic acid (OA; 18:1n-9), DHA (DHA; 22:6n-3), or arachidonic acid esters. Compounds analysis by mass spectroscopy. A carbonyl group next to the glycerol backbone in the substrate-binding pocket substantially increases the catalytic activity of PSS2. Isozyme PSS2 shows a 10fold preference for sn-1 18:0, when 22:6 is esterified at the sn-2 position. Synthesis of 18:0,22:6-PS is most efficient compared with 18:0,18:1-PS and with 18:0,20:4-PS. Substrate specificity and acyl chain preference of isozyme PS2, overview
722906
Mus musculus
?
-
-
-
-
2.7.8.29
phosphatidylethanolamine plasmalogen + L-serine
-
722906
Mus musculus
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.8.29
?
x * 50000, calculated from amino acid sequence
Mus musculus
2.7.8.29
?
x * 50000, about, recombinant FLAG-tagged PSS2, SDS-PAGE
Mus musculus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.8.29
37
-
assay at
Mus musculus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.8.29
7.4
-
assay at
Mus musculus
General Information
EC Number
General Information
Commentary
Organism
2.7.8.29
metabolism
phosphatidylserine is synthesized in mammalian cells by two integral membrane proteins, PS synthase 1 and 2 (PSS1 and PSS2). These enzymes catalyze the formation of phosphatidylserine by an exchange reaction in which serine replaces the head group of the corresponding substrate phospholipids, phosphatidylcholine (PC) for isozyme PSS1 and phosphatidylethanolamine (PE) for isozyme PSS2; phosphatidylserine is synthesized in mammalian cells by two integral membrane proteins, PS synthase 1 and 2 (PSS1 and PSS2). These enzymes catalyze the formation of phosphatidylserine by an exchange reaction in which serine replaces the head group of the corresponding substrate phospholipids, phosphatidylcholine (PC) for isozyme PSS1 and phosphatidylethanolamine (PE) for isozyme PSS2
Mus musculus
2.7.8.29
physiological function
isozyme PSS2 may play a key role in phosphatidylserine accumulation in brain and testis through high activity toward DHA-containing substrates that are abundant in these tissues; the enzyme plays a key role in phosphatidylserine accumulation in brain and testis through high activity toward docosahexaenoic acid-containing substrates
Mus musculus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.7.8.29
metabolism
phosphatidylserine is synthesized in mammalian cells by two integral membrane proteins, PS synthase 1 and 2 (PSS1 and PSS2). These enzymes catalyze the formation of phosphatidylserine by an exchange reaction in which serine replaces the head group of the corresponding substrate phospholipids, phosphatidylcholine (PC) for isozyme PSS1 and phosphatidylethanolamine (PE) for isozyme PSS2
Mus musculus
2.7.8.29
physiological function
the enzyme plays a key role in phosphatidylserine accumulation in brain and testis through high activity toward docosahexaenoic acid-containing substrates
Mus musculus
2.7.8.29
physiological function
isozyme PSS2 may play a key role in phosphatidylserine accumulation in brain and testis through high activity toward DHA-containing substrates that are abundant in these tissues
Mus musculus