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Literature summary extracted from

  • Liao, R.Z.; Yu, J.G.; Himo, F.
    Tungsten-dependent formaldehyde ferredoxin oxidoreductase: reaction mechanism from quantum chemical calculations (2011), J. Inorg. Biochem., 105, 927-936.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.B2 Tungsten tungsten-dependent enzyme. A mechanism is proposed in which the formaldehyde substrate binds directly to the tungsten ion. W(VI)=O then performs a nucleophilic attack on the formaldehyde carbon to form a tetrahedral intermediate. In the second step, which is calculated to be rate limiting, a proton is transferred to the second-shell Glu308 residue, coupled with a two-electron reduction of the tungsten ion Pyrococcus furiosus
1.2.7.5 Tungsten dependent on Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.7.5 formaldehyde + H2O + oxidized ferredoxin Pyrococcus furiosus
-
 formate + H+ + reduced ferredoxin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.B2 Pyrococcus furiosus
-
-
-
1.2.7.5 Pyrococcus furiosus Q8U1K3
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.2.7.B2 formaldehyde + H2O + 2 oxidized ferredoxin = formate + 2 H+ + 2 reduced ferredoxin a mechanism is proposed in which the formaldehyde substrate binds directly to the tungsten ion. W(VI)=O then performs a nucleophilic attack on the formaldehyde carbon to form a tetrahedral intermediate. In the second step, which is calculated to be rate limiting, a proton is transferred to the second-shell Glu308 residue, coupled with a two-electron reduction of the tungsten ion Pyrococcus furiosus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.5 formaldehyde + H2O + oxidized ferredoxin
-
 Pyrococcus furiosus formate + H+ + reduced ferredoxin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.7.5 FOR
-
 Pyrococcus furiosus
1.2.7.5 formaldehyde ferredoxin oxidoreductase
-
 Pyrococcus furiosus