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Literature summary extracted from

  • Wang, R.; Zhang, L.; Wang, D.; Gao, X.; Hong, J.
    Identification of a xylulokinase catalyzing xylulose phosphorylation in the xylose metabolic pathway of Kluyveromyces marxianus NBRC1777 (2011), J. Ind. Microbiol. Biotechnol., 38, 1739-1746.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.17 expressed in Escherichia coli BL21(DE3) cells Kluyveromyces marxianus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.17 Kluyveromyces marxianus F6JVF3
-
-
2.7.1.17 Kluyveromyces marxianus NBRC1777 F6JVF3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.17 Ni-NTA column chromatography and Sephacryl S200 gel filtration Kluyveromyces marxianus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.17 23.5
-
 using D-xylulose as substrate, 50 mM Tris-HCl (pH 7.5), at 25°C Kluyveromyces marxianus
2.7.1.17 46
-
 using D-fructose as substrate, 50 mM Tris-HCl (pH 7.5), at 25°C Kluyveromyces marxianus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.17 ATP + D-fructose
-
 Kluyveromyces marxianus ADP + D-fructose 5-phosphate
-
 ?
2.7.1.17 ATP + D-fructose
-
 Kluyveromyces marxianus NBRC1777 ADP + D-fructose 5-phosphate
-
 ?
2.7.1.17 ATP + D-xylulose
-
 Kluyveromyces marxianus ADP + D-xylulose 5-phosphate
-
 ?
2.7.1.17 ATP + D-xylulose
-
 Kluyveromyces marxianus NBRC1777 ADP + D-xylulose 5-phosphate
-
 ?
2.7.1.17 additional information no activity with D-xylose Kluyveromyces marxianus ?
-
 ?
2.7.1.17 additional information no activity with D-xylose Kluyveromyces marxianus NBRC1777 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.17 D-xylulokinase
-
 Kluyveromyces marxianus
2.7.1.17 XYL3
-
 Kluyveromyces marxianus

General Information

EC Number General Information Comment Organism
2.7.1.17 malfunction disruption of XYL3 results in both loss of xylitol utilization and marked decrease in xylose utilization Kluyveromyces marxianus
2.7.1.17 metabolism the enzyme is critical for xylose and xylitol utilization Kluyveromyces marxianus