Literature summary extracted from
Miyoshi, K.; Matsuura, H.; Kumon, T.; Matsuda, K.; Yoshikawa, T.; Bamba, T.; Harada, K.; Hirata, K.
Isolation and partial characterization of the Chlamydomonas reinhardtii gamma-glutamyl kinase (2011), J. Biosci. Bioeng., 112, 75-78.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.7.2.11 |
expressed in Escherichia coli as a His-tagged fusion protein |
Chlamydomonas reinhardtii |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
2.7.2.11 |
L-proline |
results indicate that the sensitivity of Chlamydomonas glutamte kinase to feedback inhibition by L-proline is much lower than that of similar microbial glutamte kinase |
Chlamydomonas reinhardtii |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
2.7.2.11 |
chloroplast |
computer predictions suggest that a chloroplast transit peptide exists at the N-terminal region |
Chlamydomonas reinhardtii |
9507 |
- |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
2.7.2.11 |
100000 |
- |
SDS-PAGE, recombinant protein |
Chlamydomonas reinhardtii |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.7.2.11 |
Chlamydomonas reinhardtii |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.7.2.11 |
using Ni-NTA chromatography |
Chlamydomonas reinhardtii |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.7.2.11 |
ATP + L-glutamate |
- |
Chlamydomonas reinhardtii |
ADP + L-glutamate 5-phosphate |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.7.2.11 |
gamma-glutamyl kinase |
- |
Chlamydomonas reinhardtii |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
2.7.2.11 |
37 |
- |
assay at |
Chlamydomonas reinhardtii |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
2.7.2.11 |
7 |
- |
assay at |
Chlamydomonas reinhardtii |
IC50 Value
EC Number |
IC50 Value |
IC50 Value Maximum |
Comment |
Organism |
Inhibitor |
Structure |
---|
2.7.2.11 |
90 |
- |
pH 7.0, 37°C |
Chlamydomonas reinhardtii |
L-proline |
|
General Information
EC Number |
General Information |
Comment |
Organism |
---|
2.7.2.11 |
physiological function |
Chlamydomonas reinhardtii gamma-glutamte kinase is functional as demonstrated by successful complementation of Escherichia coli auxotroph JW0232 (strain in which the proB gene has been knocked-out) |
Chlamydomonas reinhardtii |