Literature summary extracted from

Hagen, W.R.; Silva, P.J.; Amorim, M.A.; Hagedoorn, P.L.; Wassink, H.; Haaker, H.; Robb, F.T.
Novel structure and redox chemistry of the prosthetic groups of the iron-sulfur flavoprotein sulfide dehydrogenase from Pyrococcus furiosus; evidence for a [2Fe-2S] cluster with Asp(Cys)3 ligands (2000), J. Biol. Inorg. Chem., 5, 527-534.

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.8.1.19	30686	-	1 * 52598 (alpha-subunit, SudA) + 1 * 30686 (beta-subunit, SudB), calculated from sequence	Pyrococcus furiosus
1.8.1.19	52598	-	1 * 52598 (alpha-subunit, SudA) + 1 * 30686 (beta-subunit, SudB), calculated from sequence	Pyrococcus furiosus

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.8.1.19	Pyrococcus furiosus	Q8U195 and Q8U194	Q8U195: subunit alpha, and Q8U194: subunit beta	-

Subunits

EC Number	Subunits	Comment	Organism
1.8.1.19	heterodimer	1 * 52598 (alpha-subunit, SudA) + 1 * 30686 (beta-subunit, SudB), calculated from sequence	Pyrococcus furiosus

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.8.1.19	FAD	both the alpha-subunit and the beta-subunit contains one FAD	Pyrococcus furiosus
1.8.1.19	Fe-S center	the alpha-subunit carries a [2Fe-2S]2+,+ prosthetic group. It exhibits EPR g-values, 2.035, 1.908, 1.786, and reduction potential, Em,8 = +80 mV, reminiscent of Rieske-type clusters. Comparative sequence analysis indicates that this cluster is coordinated by a novel motif of one Asp and three Cys ligands. The beta-subunit carries a [3Fe-4S]+,0 cluster, and a [4Fe-4S]2+,+ cluster. The 3Fe cluster has an unusually high reduction potential, Em,8 = +230 mV. The reduced 4Fe cluster exhibits a complex EPR signal, presumably resulting from magnetic interaction of its S = 1/2 spin with the S=2 spin of the reduced 3Fe cluster. The 4Fe cluster can be reduced with deazaflavin/EDTA/light but not with sodium dithionite, however, it is readily reduced with NADPH	Pyrococcus furiosus
1.8.1.19	NADPH	both the alpha-subunit and the beta-subunit carry one putative nucleotide binding site for NADPH	Pyrococcus furiosus

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Release 2023.1 (January 2023)