BRENDA - Enzyme Database

Phosphodeoxyribosyltransferases: designed enzymes for deoxyribonucleotide synthesis

Kaminski, P.A.; Labesse, G.; J. Biol. Chem. 288, 6534-6541 (2013)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.4.2.6
-
Lactobacillus leichmannii
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.6
11
-
2'-deoxycytidine
mutant F13R/D92S/N123S, pH 6.0, 37°C
Lactobacillus leichmannii
2.4.2.6
22
-
2'-deoxycytidine
mutant F13R/D92S, pH 6.0, 37°C
Lactobacillus leichmannii
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.2.6
Lactobacillus leichmannii
Q9R5V5
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.6
2'-deoxyadenosine + 5-fluorouracil
reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S
722820
Lactobacillus leichmannii
2'-deoxy-D-ribosyl-5-fluorouracil + adenine
-
-
-
?
2.4.2.6
2'-deoxycytidine + 2,6-diaminopurine
reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S
722820
Lactobacillus leichmannii
2,6-diaminopurine 2-deoxy-D-ribose 5-phosphate + cytosine
-
-
-
?
2.4.2.6
2'-deoxycytidine + 5-bromouracil
reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S
722820
Lactobacillus leichmannii
2'-deoxy-5-bromouridine + cytosine
-
-
-
?
2.4.2.6
2'-deoxycytidine + 6-chloropurine
reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S
722820
Lactobacillus leichmannii
6-chloropurine 2-deoxy-D-ribose 5-phosphate + cytosine
-
-
-
?
2.4.2.6
2'-deoxycytidine + adenine
-
722820
Lactobacillus leichmannii
2'-deoxyadenosine + cytosine
-
-
-
?
2.4.2.6
dCMP + 2,6-dichloropurine
reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S
722820
Lactobacillus leichmannii
2,6-dichloropurin-9-yl-2'-deoxy-beta-D-ribose 5'-phosphate + cytosine
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.4.2.6
0.3
-
2'-deoxycytidine
mutant F13R/D92S, pH 6.0, 37°C
Lactobacillus leichmannii
2.4.2.6
2.1
-
2'-deoxycytidine
mutant F13R/D92S/N123S, pH 6.0, 37°C
Lactobacillus leichmannii
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.2.6
-
Lactobacillus leichmannii
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.6
11
-
2'-deoxycytidine
mutant F13R/D92S/N123S, pH 6.0, 37°C
Lactobacillus leichmannii
2.4.2.6
22
-
2'-deoxycytidine
mutant F13R/D92S, pH 6.0, 37°C
Lactobacillus leichmannii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.6
2'-deoxyadenosine + 5-fluorouracil
reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S
722820
Lactobacillus leichmannii
2'-deoxy-D-ribosyl-5-fluorouracil + adenine
-
-
-
?
2.4.2.6
2'-deoxycytidine + 2,6-diaminopurine
reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S
722820
Lactobacillus leichmannii
2,6-diaminopurine 2-deoxy-D-ribose 5-phosphate + cytosine
-
-
-
?
2.4.2.6
2'-deoxycytidine + 5-bromouracil
reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S
722820
Lactobacillus leichmannii
2'-deoxy-5-bromouridine + cytosine
-
-
-
?
2.4.2.6
2'-deoxycytidine + 6-chloropurine
reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S
722820
Lactobacillus leichmannii
6-chloropurine 2-deoxy-D-ribose 5-phosphate + cytosine
-
-
-
?
2.4.2.6
2'-deoxycytidine + adenine
-
722820
Lactobacillus leichmannii
2'-deoxyadenosine + cytosine
-
-
-
?
2.4.2.6
dCMP + 2,6-dichloropurine
reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S
722820
Lactobacillus leichmannii
2,6-dichloropurin-9-yl-2'-deoxy-beta-D-ribose 5'-phosphate + cytosine
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.4.2.6
0.3
-
2'-deoxycytidine
mutant F13R/D92S, pH 6.0, 37°C
Lactobacillus leichmannii
2.4.2.6
2.1
-
2'-deoxycytidine
mutant F13R/D92S/N123S, pH 6.0, 37°C
Lactobacillus leichmannii
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.4.2.6
0.0136
-
2'-deoxycytidine
mutant F13R/D92S, pH 6.0, 37°C
Lactobacillus leichmannii
2.4.2.6
0.192
-
2'-deoxycytidine
mutant F13R/D92S/N123S, pH 6.0, 37°C
Lactobacillus leichmannii
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.4.2.6
0.0136
-
2'-deoxycytidine
mutant F13R/D92S, pH 6.0, 37°C
Lactobacillus leichmannii
2.4.2.6
0.192
-
2'-deoxycytidine
mutant F13R/D92S/N123S, pH 6.0, 37°C
Lactobacillus leichmannii