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Literature summary extracted from

  • Moore, S.J.; Biedendieck, R.; Lawrence, A.D.; Deery, E.; Howard, M.J.; Rigby, S.E.; Warren, M.J.
    Characterization of the enzyme CbiH60 involved in anaerobic ring contraction of the cobalamin (vitamin B12) biosynthetic pathway (2013), J. Biol. Chem., 288, 297-305.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.272 expression in Bacillus megaterium DSM319 Priestia megaterium

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.272 C402A mutation introduced to disrupt the iron-sulfur center. Mutant lacks any visible coloration and has decreased absorbance between 350 and 420 nm and less than 95% of wild-type activity Priestia megaterium
2.1.1.272 C443A mutation introduced to disrupt the iron-sulfur center. Mutant lacks any visible coloration and has decreased absorbance between 350 and 420 nm and less than 95% of wild-type activity Priestia megaterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.272 65000
-
 x * 65000, SDS-PAGE of recombinant enzyme including His-tag Priestia megaterium

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.272 Priestia megaterium O87689
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.272 additional information enzyme catalyzes both methylation at C-17 and ring contraction Priestia megaterium ?
-
 ?
2.1.1.272 S-adenosyl-L-methionine + cobalt(II)-factor-III + dithiothreitol
-
 Priestia megaterium S-adenosyl-L-homocysteine + cobalt(II)-precorrin-4 + oxidized dithiothreitol
-
 ?
2.1.1.272 S-adenosyl-L-methionine + cobalt(II)-precorrin-3
-
 Priestia megaterium S-adenosyl-L-homocysteine + cobalt(II)-factor-IV
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.272 ? x * 65000, SDS-PAGE of recombinant enzyme including His-tag Priestia megaterium

Synonyms

EC Number Synonyms Comment Organism
2.1.1.272 CbiH60
-
 Priestia megaterium
2.1.1.272 Precorrin-3 methylase
-
 Priestia megaterium

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.272 [4Fe-4S]-center enzyme contains a [4Fe-4S]-center required for ring contraction reaction. Iron content is 4.8 mol of iron/mol of enzyme Priestia megaterium