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Literature summary extracted from

  • Bunker, R.D.; Bulloch, E.M.; Dickson, J.M.; Loomes, K.M.; Baker, E.N.
    Structure and function of human xylulokinase, an enzyme with important roles in carbohydrate metabolism (2013), J. Biol. Chem., 288, 1643-1652.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.17 expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.17 sitting drop vapor diffusion method, using 200 mM MES-KOH, pH 6.0, 16% (w/v) polyethylene glycol 6000, 0.3 mM NaN3 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.17 5-deoxy-5-fluoro-D-xylulose
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.17 0.024
-
 D-xylulose in 50 mM NH4HCO3, pH 8.0, 10% (v/v) D2O, 10 mM MgCl2 at 25°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.17 Homo sapiens O75191
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.17 apo-enzyme and in complex with D-xylulose, ATP plus D-xylulose or 5-deoxy-5-fluoro-D-xylulose, nickel affinity column chromatography and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.17 ATP + D-xylulose
-
 Homo sapiens ADP + D-xylulose 5-phosphate
-
 ?
2.7.1.17 additional information D-ribulose is not a substrate Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.17 D-xylulokinase
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.17 35
-
 D-xylulose in 50 mM NH4HCO3, pH 8.0, 10% (v/v) D2O, 10 mM MgCl2 at 25°C Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.17 0.025
-
 5-deoxy-5-fluoro-D-xylulose in 50 mM NH4HCO3, pH 8.0, 10% (v/v) D2O, 10 mM MgCl2 at 25°C Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.17 1500
-
 D-xylulose in 50 mM NH4HCO3, pH 8.0, 10% (v/v) D2O, 10 mM MgCl2 at 25°C Homo sapiens