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Literature summary extracted from

  • Mathieu, Y.; Prosper, P.; Buee, M.; Dumarcay, S.; Favier, F.; Gelhaye, E.; Gerardin, P.; Harvengt, L.; Jacquot, J.P.; Lamant, T.; Meux, E.; Mathiot, S.; Didierjean, C.; Morel, M.
    Characterization of a Phanerochaete chrysosporium glutathione transferase reveals a novel structural and functional class with ligandin properties (2012), J. Biol. Chem., 287, 39001-39011.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.18 expression of wild-type and mutant GST5118 in Escherichia coli strain BL21(DE3), production of the selenomethionine-substituted GST5118 in a methionine auxotroph strain BL21(DE3)Met- Phanerodontia chrysosporium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.18 purified recombinant selenomethionine-apoGST5118 and native holoGST5118 with bound glutathione, microbatch under oil, paraffin, method at 4°C, 4 Selenomethionine-apoGST5118 crystals appear after 4-5 days from droplets containing 0.002 ml of 15-20 mg/ml protein in TE buffer, and 0.002 ml of precipitating solution containing 30% PEG 8000, 200 mM sodium acetate,and 100 mM sodium cacodylate, pH 6.5. The best crystals of GST5118 in complex with GSH grow after 2 months in droplets containing a mixture of 14 mg/ml protein and 6 mM GSH in TE buffer and precipitating solution containing 30% PEG 8000, 200 mM sodium acetate, and 100 mM HEPES, pH 7.0. X-ray diffraction structure determination and analysis at 1.8-2.0 A resolution, single-wavelength anomalous dispersion, the molecular replacement method fails to solve the protein structure Phanerodontia chrysosporium

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.18 S22A site-directed mutagenesis Phanerodontia chrysosporium
2.5.1.18 S22C site-directed mutagenesis Phanerodontia chrysosporium

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.18 Vanillin
-
 Phanerodontia chrysosporium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 additional information
-
 additional information GST5118 kinetic mechanism, overview Phanerodontia chrysosporium
2.5.1.18 0.119
-
 phenethyl isothiocyanate recombinant wild-type, pH 6.5, temperature not specified in the publication Phanerodontia chrysosporium
2.5.1.18 0.191
-
 4-nitrophenyl butyrate recombinant mutant S22A, pH 6.5, temperature not specified in the publication Phanerodontia chrysosporium
2.5.1.18 0.216
-
 glutathione recombinant wild-type, pH 6.5, temperature not specified in the publication Phanerodontia chrysosporium
2.5.1.18 0.615
-
 glutathione recombinant mutant S22A, pH 6.5, temperature not specified in the publication Phanerodontia chrysosporium
2.5.1.18 0.654
-
 1-chloro-2,4-dinitrobenzene recombinant mutant S22A, pH 6.5, temperature not specified in the publication Phanerodontia chrysosporium
2.5.1.18 0.774
-
 4-nitrophenyl butyrate recombinant wild-type, pH 6.5, temperature not specified in the publication Phanerodontia chrysosporium

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Phanerodontia chrysosporium I6ZUH3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 recombinant wild-type and mutant GST5118 from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography, followed by dialysis and ultrafiltration Phanerodontia chrysosporium

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.18 RX + glutathione = HX + R-S-glutathione substrate binding structure and reaction mechanism, conformational changes, detailed overview Phanerodontia chrysosporium
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 1-chloro-2,4-dinitrobenzene + glutathione
-
 Phanerodontia chrysosporium S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?
2.5.1.18 4-nitrophenyl butyrate + glutathione
-
 Phanerodontia chrysosporium S-(4-nitrophenyl)glutathione + butyrate
-
 ?
2.5.1.18 additional information GST5118 binds glutathionylated and sulfated compounds but also wood extractive molecules, such as vanillin, chloronitrobenzoic acid, hydroxyacetophenone, catechins, and aldehydes, in the glutathione pocket, overview. Glutathione binding structure and mechanism, detailed overview. The enzyme also shows peroxidase activity Phanerodontia chrysosporium ?
-
 ?
2.5.1.18 phenethyl isothiocyanate + glutathione
-
 Phanerodontia chrysosporium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.18 homodimer the GST5118 monomer adopts the GST canonical fold, which consists of an N-terminal thioredoxin-like motif and a C-terminal domain of at least four helices, unique dimeric organization of GST5118, overview Phanerodontia chrysosporium

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 glutathione S-transferase
-
 Phanerodontia chrysosporium
2.5.1.18 GST
-
 Phanerodontia chrysosporium
2.5.1.18 GST5118
-
 Phanerodontia chrysosporium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.18 0.23
-
 glutathione recombinant mutant S22A, pH 6.5, temperature not specified in the publication Phanerodontia chrysosporium
2.5.1.18 0.29
-
 4-nitrophenyl butyrate recombinant mutant S22A, pH 6.5, temperature not specified in the publication Phanerodontia chrysosporium
2.5.1.18 8.52
-
 phenethyl isothiocyanate recombinant wild-type, pH 6.5, temperature not specified in the publication Phanerodontia chrysosporium
2.5.1.18 11.03
-
 glutathione recombinant wild-type, pH 6.5, temperature not specified in the publication Phanerodontia chrysosporium
2.5.1.18 27.6
-
 4-nitrophenyl butyrate recombinant wild-type, pH 6.5, temperature not specified in the publication Phanerodontia chrysosporium
2.5.1.18 29.42
-
 1-chloro-2,4-dinitrobenzene recombinant mutant S22A, pH 6.5, temperature not specified in the publication Phanerodontia chrysosporium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.18 6.5 8 assay at Phanerodontia chrysosporium

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.18 0.16
-
 Vanillin pH 6.5, temperature not specified in the publication Phanerodontia chrysosporium

General Information

EC Number General Information Comment Organism
2.5.1.18 evolution GST5118 belongs to a distinct class named GSTFuA, for fungal specific GST class A, as part of the GST superfamily Phanerodontia chrysosporium
2.5.1.18 physiological function glutathione S-transferases form a superfamily of multifunctional proteins with essential roles in cellular detoxification processes. GST5118 of Phanerochaete chrysosporium can function as a classical GST through the addition of glutathione mainly to phenethyl isothiocyanate, but alternatively and in a competitive way, it can also act as a ligandin of wood extractive compounds Phanerodontia chrysosporium

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.18 0.37
-
 glutathione recombinant mutant S22A, pH 6.5, temperature not specified in the publication Phanerodontia chrysosporium
2.5.1.18 1.53
-
 4-nitrophenyl butyrate recombinant mutant S22A, pH 6.5, temperature not specified in the publication Phanerodontia chrysosporium
2.5.1.18 3.5
-
 4-nitrophenyl butyrate recombinant wild-type, pH 6.5, temperature not specified in the publication Phanerodontia chrysosporium
2.5.1.18 44.83
-
 1-chloro-2,4-dinitrobenzene recombinant mutant S22A, pH 6.5, temperature not specified in the publication Phanerodontia chrysosporium
2.5.1.18 51.67
-
 glutathione recombinant wild-type, pH 6.5, temperature not specified in the publication Phanerodontia chrysosporium
2.5.1.18 71.67
-
 phenethyl isothiocyanate recombinant wild-type, pH 6.5, temperature not specified in the publication Phanerodontia chrysosporium