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Literature summary extracted from

  • Chandrayan, S.K.; McTernan, P.M.; Hopkins, R.C.; Sun, J.; Jenney, F.E.; Adams, M.W.
    Engineering hyperthermophilic archaeon Pyrococcus furiosus to overproduce its cytoplasmic [NiFe]-hydrogenase (2012), J. Biol. Chem., 287, 3257-3264.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.12.1.5 synthesis the recombinant enzyme is efficient in vitro biohydrogen production Pyrococcus furiosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.12.1.5 cytoplasm
-
 Pyrococcus furiosus 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.1.5 Iron contains 1 nickel-iron catalytic site and 6 iron-sulfur clusters, contains 23 iron atoms/heterotetramer Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
1.12.1.5 Pyrococcus furiosus
-
-
-
1.12.1.5 Pyrococcus furiosus COM1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.1.5 Strep-tag II column chromatography Pyrococcus furiosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.1.5 1.23
-
 crude native enzyme, using methyl viologen as cosubstrate, at pH 8.4 and 80°C Pyrococcus furiosus
1.12.1.5 7.96
-
 crude recombinant enzyme, using methyl viologen as cosubstrate, at pH 8.4 and 80°C Pyrococcus furiosus
1.12.1.5 126
-
 recombinant enzyme after 24fold purification, using methyl viologen as cosubstrate, at pH 8.4 and 80°C Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.1.5 H+ + NADPH
-
 Pyrococcus furiosus H2 + NADP+
-
 r
1.12.1.5 H+ + NADPH
-
 Pyrococcus furiosus COM1 H2 + NADP+
-
 r
1.12.1.5 H+ + reduced methyl viologen
-
 Pyrococcus furiosus H2 + oxidized methyl viologen
-
 ?
1.12.1.5 H+ + reduced methyl viologen
-
 Pyrococcus furiosus COM1 H2 + oxidized methyl viologen
-
 ?

Subunits

EC Number Subunits Comment Organism
1.12.1.5 heterotetramer
-
 Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
1.12.1.5 SHI
-
 Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.12.1.5 25 90 the native enzyme shows half-lives of 14 h at 90°C under argon (H2 evolution), 21 h at 25°C under air (H2 evolution), and 10 h at 90°C under argon (H2 oxidation). The recombinant enzyme shows half-lives of 6 h at 90°C under argon (H2 evolution), 25 h at 25°C under air (H2 evolution), and 5 h at 90°C under argon (H2 oxidation) Pyrococcus furiosus

Cofactor

EC Number Cofactor Comment Organism Structure
1.12.1.5 flavin
-
 Pyrococcus furiosus
1.12.1.5 NADPH dependent on Pyrococcus furiosus
1.12.1.5 [2Fe-2S]-center contains 1 [2Fe-2S] centre Pyrococcus furiosus
1.12.1.5 [4Fe-4S]-center contains 2 [4Fe-4S] centres Pyrococcus furiosus