Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chen, C.; Thompson, D.A.; Koutalos, Y.
    Reduction of all-trans-retinal in vertebrate rod photoreceptors requires the combined action of RDH8 and RDH12 (2012), J. Biol. Chem., 287, 24662-24670.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.105 photoreceptor outer segment
-
 Mus musculus 1750
-
1.1.1.300 photoreceptor inner segment
-
 Mus musculus 1917
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.105 all-trans-retinol-[cellular-retinol-binding-protein] + NAD+ Mus musculus
-
 all-trans-retinal-[cellular-retinol-binding-protein] + NADH + H+
-
 ?
1.1.1.105 all-trans-retinol-[cellular-retinol-binding-protein] + NAD+ Mus musculus C57BL/6 x 129/Sv
-
 all-trans-retinal-[cellular-retinol-binding-protein] + NADH + H+
-
 ?
1.1.1.300 all-trans-retinol + NADP+ Mus musculus
-
 all-trans-retinal + NADPH + H+
-
 ?
1.1.1.300 all-trans-retinol + NADP+ Mus musculus C57BL/6 x 129/Sv
-
 all-trans-retinal + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.105 Mus musculus
-
-
-
1.1.1.105 Mus musculus C57BL/6 x 129/Sv
-
-
-
1.1.1.300 Mus musculus
-
-
-
1.1.1.300 Mus musculus C57BL/6 x 129/Sv
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.105 eye RDH8 localizes to photoreceptor outer segments Mus musculus
-
1.1.1.105 retina
-
 Mus musculus
-
1.1.1.300 eye RDH12 localizes to photoreceptor inner segments Mus musculus
-
1.1.1.300 retina
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.105 all-trans-retinol-[cellular-retinol-binding-protein] + NAD+
-
 Mus musculus all-trans-retinal-[cellular-retinol-binding-protein] + NADH + H+
-
 ?
1.1.1.105 all-trans-retinol-[cellular-retinol-binding-protein] + NAD+
-
 Mus musculus C57BL/6 x 129/Sv all-trans-retinal-[cellular-retinol-binding-protein] + NADH + H+
-
 ?
1.1.1.105 additional information no activity with decanal Mus musculus ?
-
 ?
1.1.1.105 additional information no activity with decanal Mus musculus C57BL/6 x 129/Sv ?
-
 ?
1.1.1.300 all-trans-retinol + NADP+
-
 Mus musculus all-trans-retinal + NADPH + H+
-
 ?
1.1.1.300 all-trans-retinol + NADP+
-
 Mus musculus C57BL/6 x 129/Sv all-trans-retinal + NADPH + H+
-
 ?
1.1.1.300 additional information no activity with decanal Mus musculus ?
-
 ?
1.1.1.300 additional information no activity with decanal Mus musculus C57BL/6 x 129/Sv ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.105 RDH8
-
 Mus musculus
1.1.1.105 retinoid dehydrogenase/reductase
-
 Mus musculus
1.1.1.300 RDH12
-
 Mus musculus
1.1.1.300 retinoid dehydrogenase/reductase
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.105 37
-
 assay at Mus musculus
1.1.1.300 37
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.105 7.4
-
 assay at Mus musculus
1.1.1.300 7.4
-
 assay at Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.105 NAD+
-
 Mus musculus
1.1.1.300 NADP+
-
 Mus musculus

General Information

EC Number General Information Comment Organism
1.1.1.105 evolution RDHs that catalyze the interconversion of retinal and retinol involved in rhodopsin turnover are members of the family of short chain dehydrogenase/reductases Mus musculus
1.1.1.105 malfunction outer segments of rods deficient in Rdh8 fail to reduce all-trans-retinal. Following exposure to light, a leak of retinoids from outer to inner segments is detected in rods from both wild-type and knock-out mice. In cells lacking Rdh8 or Rdh12, EC 1.1.1.300, this leak is mainly all-trans-retinal, overview. Retinal reductase activity is lost in RDH12-deficient mutants Mus musculus
1.1.1.105 physiological function in vertebrate rod cells, retinoid dehydrogenases/reductases are critical for reducing the reactive aldehyde all-trans-retinal that is released by photoactivated rhodopsin, to all-trans-retinol. Reduction of all-trans-retinal in vertebrate rod photoreceptors requires the combined action of RDH8 and RDH12. RDH8 in the outer segment provides most of the activity needed to reduce all-trans-retinal generated by the light response, overview Mus musculus
1.1.1.300 evolution RDHs that catalyze the interconversion of retinal and retinol involved in rhodopsin turnover are members of the family of short chain dehydrogenase/reductases Mus musculus
1.1.1.300 malfunction loss-of-function mutations of RDH12 cause retinal degeneration in some forms of Leber congenital amaurosis. Outer segments of rods deficient in Rdh12 show no altered phenotype. Following exposure to light, a leak of retinoids from outer to inner segments is detected in rods from both wild-type and knock-out mice. In cells lacking Rdh8, EC 1.1.1.105, or Rdh12, this leak is mainly all-trans-retinal, overview. Retinal reductase activity is lost in RDH8-deficient mutants Mus musculus
1.1.1.300 physiological function RDH12 activity in the photoreceptor inner segments is also key enzyme function. RDH12 in inner segments can protect vital cell organelles against aldehyde toxicity caused by an intracellular leak of all-trans-retinal, as well as other aldehydes originating both inside and outside the cell Mus musculus