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Literature summary extracted from

  • Suzuki, H.; Ohnishi, Y.; Furusho, Y.; Sakuda, S.; Horinouchi, S.
    Novel benzene ring biosynthesis from C3 and C4 primary metabolites by two enzymes (2006), J. Biol. Chem., 281, 36944-36951.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.56 expression in Escherichia coli Streptomyces griseus
4.1.99.20 expression in Escherichia coli Streptomyces griseus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.99.20 pyridoxal 5'-phosphate
-
 Streptomyces griseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.56 0.0056
-
 L-aspartate 4-semialdehyde pH 7.2, 30°C Streptomyces griseus
4.1.2.56 0.14
-
 glycerone phosphate pH 7.2, 30°C Streptomyces griseus
4.1.99.20 0.012
-
 2-amino-4,5-dihydroxy-6-oxo-7-(phosphonooxy)heptanoate 30°C, pH 7.2 Streptomyces griseus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.99.20 Co2+ 0.1 mM, divalent mtal ion is essential for activity. Co2+ show 85% of the activity compared to Mn2+ Streptomyces griseus
4.1.99.20 Fe2+ 0.1 mM, divalent mtal ion is essential for activity. Fe2+ show 81% of the activity compared to Mn2+ Streptomyces griseus
4.1.99.20 Mg2+ 0.1 mM, divalent mtal ion is essential for activity. Mg2+ show 41% of the activity compared to Mn2+ Streptomyces griseus
4.1.99.20 Mn2+ 0.1 mM, divalent mtal ion is essential for activity. Co2+, Fe2+ and Mg2+ can be used to a lesser extent than Mn2+ Streptomyces griseus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.56 30000
-
 gel filtration Streptomyces griseus
4.1.2.56 33000
-
 10 * 33000, SDS-PAGE Streptomyces griseus
4.1.99.20 46000
-
 1 * 46000, SDS-PAGE Streptomyces griseus
4.1.99.20 54000
-
 gel filtration Streptomyces griseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.10.3.15 3-amino-4-hydroxybenzoate + N-acetyl-L-cysteine + O2 Streptomyces griseus
-
 grixazone B + H2O + CO2
-
 ?
1.10.3.15 3-amino-4-hydroxybenzoate + N-acetyl-L-cysteine + O2 Streptomyces griseus IFO 13350
-
 grixazone B + H2O + CO2
-
 ?
4.1.2.56 glycerone phosphate + L-aspartate 4-semialdehyde Streptomyces griseus
-
 2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate
-
 ?
4.1.2.56 glycerone phosphate + L-aspartate 4-semialdehyde Streptomyces griseus TK21
-
 2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate
-
 ?
4.1.99.20 2-amino-4,5-dihydroxy-6-oxo-7-(phosphonooxy)heptanoate Streptomyces griseus the enzyme is involved in biosynthesis of grixazone, a yellow pigment that contains a phenoxazinone chromophore 3-amino-4-hydroxybenzoate + phosphate + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.15 Streptomyces griseus
-
-
-
1.10.3.15 Streptomyces griseus IFO 13350
-
-
-
4.1.2.56 Streptomyces griseus A0JC77
-
-
4.1.2.56 Streptomyces griseus TK21 A0JC77
-
-
4.1.99.20 Streptomyces griseus A0JC76
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.56
-
 Streptomyces griseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.15 3-amino-4-hydroxybenzoate + N-acetyl-L-cysteine + O2
-
 Streptomyces griseus grixazone B + H2O + CO2
-
 ?
1.10.3.15 3-amino-4-hydroxybenzoate + N-acetyl-L-cysteine + O2
-
 Streptomyces griseus IFO 13350 grixazone B + H2O + CO2
-
 ?
4.1.2.56 glycerone phosphate + L-aspartate 4-semialdehyde
-
 Streptomyces griseus 2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate
-
 ?
4.1.2.56 glycerone phosphate + L-aspartate 4-semialdehyde part of the pathway for the biosynthesis of grixazone, a mixture of yellow pigments produced by the bacterium Streptomyces griseus Streptomyces griseus 2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate
-
 ?
4.1.2.56 glycerone phosphate + L-aspartate 4-semialdehyde
-
 Streptomyces griseus TK21 2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate
-
 ?
4.1.2.56 glycerone phosphate + L-aspartate 4-semialdehyde part of the pathway for the biosynthesis of grixazone, a mixture of yellow pigments produced by the bacterium Streptomyces griseus Streptomyces griseus TK21 2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate
-
 ?
4.1.99.20 2-amino-4,5-dihydroxy-6-oxo-7-(phosphonooxy)heptanoate the enzyme is involved in biosynthesis of grixazone, a yellow pigment that contains a phenoxazinone chromophore Streptomyces griseus 3-amino-4-hydroxybenzoate + phosphate + 2 H2O
-
 ?
4.1.99.20 2-amino-4,5-dihydroxy-6-oxo-7-(phosphonooxy)heptanoate the reaction is suggested to take place in several steps. Schiff base formation, double bond migration and dephosphorylation followed by ring opening and closing to form a pyrrolidine ring, and finally dehydration to form the product 3-amino-4-hydroxybenzoate Streptomyces griseus 3-amino-4-hydroxybenzoate + phosphate + 2 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.56 decamer 10 * 33000, SDS-PAGE Streptomyces griseus
4.1.99.20 monomer 1 * 46000, SDS-PAGE Streptomyces griseus

Synonyms

EC Number Synonyms Comment Organism
4.1.2.56 griI
-
 Streptomyces griseus
4.1.99.20 3,4-AHBA synthase
-
 Streptomyces griseus
4.1.99.20 griH
-
 Streptomyces griseus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.56 30
-
 assay at Streptomyces griseus
4.1.99.20 30
-
-
 Streptomyces griseus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.2.56 40
-
 stable below Streptomyces griseus
4.1.99.20 30
-
 at pH 7.2, 1 h, stable below Streptomyces griseus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.56 0.2
-
 glycerone phosphate pH 7.2, 30°C Streptomyces griseus
4.1.2.56 0.2
-
 L-aspartate 4-semialdehyde pH 7.2, 30°C Streptomyces griseus
4.1.99.20 0.025
-
 2-amino-4,5-dihydroxy-6-oxo-7-(phosphonooxy)heptanoate 30°C, pH 7.2 Streptomyces griseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.56 8
-
-
 Streptomyces griseus
4.1.99.20 8
-
-
 Streptomyces griseus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.99.20 7.5 9 pH 7.5: about 75% of maximal activity, pH 9.0: about 75% of maximal activity Streptomyces griseus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.2.56 6.5 10 1 h, 30°C, stable Streptomyces griseus
4.1.99.20 6.5 10.5 at 30°C, stable for 1 h Streptomyces griseus

General Information

EC Number General Information Comment Organism
4.1.2.56 physiological function part of the pathway for the biosynthesis of grixazone, a mixture of yellow pigments produced by the bacterium Streptomyces griseus Streptomyces griseus