BRENDA - Enzyme Database show

Novel benzene ring biosynthesis from C3 and C4 primary metabolites by two enzymes

Suzuki, H.; Ohnishi, Y.; Furusho, Y.; Sakuda, S.; Horinouchi, S.; J. Biol. Chem. 281, 36944-36951 (2006)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.1.2.56
expression in Escherichia coli
Streptomyces griseus
4.1.99.20
expression in Escherichia coli
Streptomyces griseus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.99.20
pyridoxal 5'-phosphate
-
Streptomyces griseus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.2.56
0.0056
-
L-aspartate 4-semialdehyde
pH 7.2, 30°C
Streptomyces griseus
4.1.2.56
0.14
-
glycerone phosphate
pH 7.2, 30°C
Streptomyces griseus
4.1.99.20
0.012
-
2-amino-4,5-dihydroxy-6-oxo-7-(phosphonooxy)heptanoate
30°C, pH 7.2
Streptomyces griseus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.99.20
Co2+
0.1 mM, divalent mtal ion is essential for activity. Co2+ show 85% of the activity compared to Mn2+
Streptomyces griseus
4.1.99.20
Fe2+
0.1 mM, divalent mtal ion is essential for activity. Fe2+ show 81% of the activity compared to Mn2+
Streptomyces griseus
4.1.99.20
Mg2+
0.1 mM, divalent mtal ion is essential for activity. Mg2+ show 41% of the activity compared to Mn2+
Streptomyces griseus
4.1.99.20
Mn2+
0.1 mM, divalent mtal ion is essential for activity. Co2+, Fe2+ and Mg2+ can be used to a lesser extent than Mn2+
Streptomyces griseus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.2.56
30000
-
gel filtration
Streptomyces griseus
4.1.2.56
33000
-
10 * 33000, SDS-PAGE
Streptomyces griseus
4.1.99.20
46000
-
1 * 46000, SDS-PAGE
Streptomyces griseus
4.1.99.20
54000
-
gel filtration
Streptomyces griseus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.10.3.15
3-amino-4-hydroxybenzoate + N-acetyl-L-cysteine + O2
Streptomyces griseus
-
grixazone B + H2O + CO2
-
-
?
1.10.3.15
3-amino-4-hydroxybenzoate + N-acetyl-L-cysteine + O2
Streptomyces griseus IFO 13350
-
grixazone B + H2O + CO2
-
-
?
4.1.2.56
glycerone phosphate + L-aspartate 4-semialdehyde
Streptomyces griseus
-
2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate
-
-
?
4.1.2.56
glycerone phosphate + L-aspartate 4-semialdehyde
Streptomyces griseus TK21
-
2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate
-
-
?
4.1.99.20
2-amino-4,5-dihydroxy-6-oxo-7-(phosphonooxy)heptanoate
Streptomyces griseus
the enzyme is involved in biosynthesis of grixazone, a yellow pigment that contains a phenoxazinone chromophore
3-amino-4-hydroxybenzoate + phosphate + 2 H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.10.3.15
Streptomyces griseus
-
-
-
1.10.3.15
Streptomyces griseus IFO 13350
-
-
-
4.1.2.56
Streptomyces griseus
A0JC77
-
-
4.1.2.56
Streptomyces griseus TK21
A0JC77
-
-
4.1.99.20
Streptomyces griseus
A0JC76
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.2.56
-
Streptomyces griseus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.10.3.15
3-amino-4-hydroxybenzoate + N-acetyl-L-cysteine + O2
-
722650
Streptomyces griseus
grixazone B + H2O + CO2
-
-
-
?
1.10.3.15
3-amino-4-hydroxybenzoate + N-acetyl-L-cysteine + O2
-
722650
Streptomyces griseus IFO 13350
grixazone B + H2O + CO2
-
-
-
?
4.1.2.56
glycerone phosphate + L-aspartate 4-semialdehyde
-
722650
Streptomyces griseus
2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate
-
-
-
?
4.1.2.56
glycerone phosphate + L-aspartate 4-semialdehyde
part of the pathway for the biosynthesis of grixazone, a mixture of yellow pigments produced by the bacterium Streptomyces griseus
722650
Streptomyces griseus
2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate
-
-
-
?
4.1.2.56
glycerone phosphate + L-aspartate 4-semialdehyde
-
722650
Streptomyces griseus TK21
2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate
-
-
-
?
4.1.2.56
glycerone phosphate + L-aspartate 4-semialdehyde
part of the pathway for the biosynthesis of grixazone, a mixture of yellow pigments produced by the bacterium Streptomyces griseus
722650
Streptomyces griseus TK21
2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate
-
-
-
?
4.1.99.20
2-amino-4,5-dihydroxy-6-oxo-7-(phosphonooxy)heptanoate
the enzyme is involved in biosynthesis of grixazone, a yellow pigment that contains a phenoxazinone chromophore
722650
Streptomyces griseus
3-amino-4-hydroxybenzoate + phosphate + 2 H2O
-
-
-
?
4.1.99.20
2-amino-4,5-dihydroxy-6-oxo-7-(phosphonooxy)heptanoate
the reaction is suggested to take place in several steps. Schiff base formation, double bond migration and dephosphorylation followed by ring opening and closing to form a pyrrolidine ring, and finally dehydration to form the product 3-amino-4-hydroxybenzoate
722650
Streptomyces griseus
3-amino-4-hydroxybenzoate + phosphate + 2 H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.1.2.56
decamer
10 * 33000, SDS-PAGE
Streptomyces griseus
4.1.99.20
monomer
1 * 46000, SDS-PAGE
Streptomyces griseus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.1.2.56
30
-
assay at
Streptomyces griseus
4.1.99.20
30
-
-
Streptomyces griseus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.1.2.56
40
-
stable below
Streptomyces griseus
4.1.99.20
30
-
at pH 7.2, 1 h, stable below
Streptomyces griseus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.1.2.56
0.2
-
glycerone phosphate
pH 7.2, 30°C
Streptomyces griseus
4.1.2.56
0.2
-
L-aspartate 4-semialdehyde
pH 7.2, 30°C
Streptomyces griseus
4.1.99.20
0.025
-
2-amino-4,5-dihydroxy-6-oxo-7-(phosphonooxy)heptanoate
30°C, pH 7.2
Streptomyces griseus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.2.56
8
-
-
Streptomyces griseus
4.1.99.20
8
-
-
Streptomyces griseus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.1.99.20
7.5
9
pH 7.5: about 75% of maximal activity, pH 9.0: about 75% of maximal activity
Streptomyces griseus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
4.1.2.56
6.5
10
1 h, 30°C, stable
Streptomyces griseus
4.1.99.20
6.5
10.5
at 30°C, stable for 1 h
Streptomyces griseus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.2.56
expression in Escherichia coli
Streptomyces griseus
4.1.99.20
expression in Escherichia coli
Streptomyces griseus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.99.20
pyridoxal 5'-phosphate
-
Streptomyces griseus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.2.56
0.0056
-
L-aspartate 4-semialdehyde
pH 7.2, 30°C
Streptomyces griseus
4.1.2.56
0.14
-
glycerone phosphate
pH 7.2, 30°C
Streptomyces griseus
4.1.99.20
0.012
-
2-amino-4,5-dihydroxy-6-oxo-7-(phosphonooxy)heptanoate
30°C, pH 7.2
Streptomyces griseus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.99.20
Co2+
0.1 mM, divalent mtal ion is essential for activity. Co2+ show 85% of the activity compared to Mn2+
Streptomyces griseus
4.1.99.20
Fe2+
0.1 mM, divalent mtal ion is essential for activity. Fe2+ show 81% of the activity compared to Mn2+
Streptomyces griseus
4.1.99.20
Mg2+
0.1 mM, divalent mtal ion is essential for activity. Mg2+ show 41% of the activity compared to Mn2+
Streptomyces griseus
4.1.99.20
Mn2+
0.1 mM, divalent mtal ion is essential for activity. Co2+, Fe2+ and Mg2+ can be used to a lesser extent than Mn2+
Streptomyces griseus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.2.56
30000
-
gel filtration
Streptomyces griseus
4.1.2.56
33000
-
10 * 33000, SDS-PAGE
Streptomyces griseus
4.1.99.20
46000
-
1 * 46000, SDS-PAGE
Streptomyces griseus
4.1.99.20
54000
-
gel filtration
Streptomyces griseus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.10.3.15
3-amino-4-hydroxybenzoate + N-acetyl-L-cysteine + O2
Streptomyces griseus
-
grixazone B + H2O + CO2
-
-
?
1.10.3.15
3-amino-4-hydroxybenzoate + N-acetyl-L-cysteine + O2
Streptomyces griseus IFO 13350
-
grixazone B + H2O + CO2
-
-
?
4.1.2.56
glycerone phosphate + L-aspartate 4-semialdehyde
Streptomyces griseus
-
2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate
-
-
?
4.1.2.56
glycerone phosphate + L-aspartate 4-semialdehyde
Streptomyces griseus TK21
-
2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate
-
-
?
4.1.99.20
2-amino-4,5-dihydroxy-6-oxo-7-(phosphonooxy)heptanoate
Streptomyces griseus
the enzyme is involved in biosynthesis of grixazone, a yellow pigment that contains a phenoxazinone chromophore
3-amino-4-hydroxybenzoate + phosphate + 2 H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.2.56
-
Streptomyces griseus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.10.3.15
3-amino-4-hydroxybenzoate + N-acetyl-L-cysteine + O2
-
722650
Streptomyces griseus
grixazone B + H2O + CO2
-
-
-
?
1.10.3.15
3-amino-4-hydroxybenzoate + N-acetyl-L-cysteine + O2
-
722650
Streptomyces griseus IFO 13350
grixazone B + H2O + CO2
-
-
-
?
4.1.2.56
glycerone phosphate + L-aspartate 4-semialdehyde
-
722650
Streptomyces griseus
2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate
-
-
-
?
4.1.2.56
glycerone phosphate + L-aspartate 4-semialdehyde
part of the pathway for the biosynthesis of grixazone, a mixture of yellow pigments produced by the bacterium Streptomyces griseus
722650
Streptomyces griseus
2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate
-
-
-
?
4.1.2.56
glycerone phosphate + L-aspartate 4-semialdehyde
-
722650
Streptomyces griseus TK21
2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate
-
-
-
?
4.1.2.56
glycerone phosphate + L-aspartate 4-semialdehyde
part of the pathway for the biosynthesis of grixazone, a mixture of yellow pigments produced by the bacterium Streptomyces griseus
722650
Streptomyces griseus TK21
2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate
-
-
-
?
4.1.99.20
2-amino-4,5-dihydroxy-6-oxo-7-(phosphonooxy)heptanoate
the enzyme is involved in biosynthesis of grixazone, a yellow pigment that contains a phenoxazinone chromophore
722650
Streptomyces griseus
3-amino-4-hydroxybenzoate + phosphate + 2 H2O
-
-
-
?
4.1.99.20
2-amino-4,5-dihydroxy-6-oxo-7-(phosphonooxy)heptanoate
the reaction is suggested to take place in several steps. Schiff base formation, double bond migration and dephosphorylation followed by ring opening and closing to form a pyrrolidine ring, and finally dehydration to form the product 3-amino-4-hydroxybenzoate
722650
Streptomyces griseus
3-amino-4-hydroxybenzoate + phosphate + 2 H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.2.56
decamer
10 * 33000, SDS-PAGE
Streptomyces griseus
4.1.99.20
monomer
1 * 46000, SDS-PAGE
Streptomyces griseus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.1.2.56
30
-
assay at
Streptomyces griseus
4.1.99.20
30
-
-
Streptomyces griseus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.1.2.56
40
-
stable below
Streptomyces griseus
4.1.99.20
30
-
at pH 7.2, 1 h, stable below
Streptomyces griseus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.1.2.56
0.2
-
glycerone phosphate
pH 7.2, 30°C
Streptomyces griseus
4.1.2.56
0.2
-
L-aspartate 4-semialdehyde
pH 7.2, 30°C
Streptomyces griseus
4.1.99.20
0.025
-
2-amino-4,5-dihydroxy-6-oxo-7-(phosphonooxy)heptanoate
30°C, pH 7.2
Streptomyces griseus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.2.56
8
-
-
Streptomyces griseus
4.1.99.20
8
-
-
Streptomyces griseus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.1.99.20
7.5
9
pH 7.5: about 75% of maximal activity, pH 9.0: about 75% of maximal activity
Streptomyces griseus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
4.1.2.56
6.5
10
1 h, 30°C, stable
Streptomyces griseus
4.1.99.20
6.5
10.5
at 30°C, stable for 1 h
Streptomyces griseus
General Information
EC Number
General Information
Commentary
Organism
4.1.2.56
physiological function
part of the pathway for the biosynthesis of grixazone, a mixture of yellow pigments produced by the bacterium Streptomyces griseus
Streptomyces griseus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
4.1.2.56
physiological function
part of the pathway for the biosynthesis of grixazone, a mixture of yellow pigments produced by the bacterium Streptomyces griseus
Streptomyces griseus