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Literature summary extracted from

  • Gueguen, Y.; Voorhorst, W.G.; van der Oost, J.; de Vos, W.M.
    Molecular and biochemical characterization of an endo-beta-1,3-glucanase of the hyperthermophilic archaeon Pyrococcus furiosus (1997), J. Biol. Chem., 272, 31258-31264.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.39 overexpressed in Escherichia coli using the T7-expression system Pyrococcus furiosus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.39 delMet174 the specific activities of the mutant LamA, on both laminarin and lichenan, is about 10fold lower than the respective wild-type value. The methionine deletion leads to an enzyme with 13.1% of wild-type Vmax and a slightly higher Km on laminarin as a substrate. With lichenan as substrate, the mutant LamA has 7.9% of the wild-type Vmax and the same Km value Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.39 additional information
-
 Laminarin 2.8 mg/ml, pH 6.5, 80°C, wild-type enzyme. 3.5 mg/ml, pH 6.5, 80°C, mutant enzyme delMet174 Pyrococcus furiosus
3.2.1.39 additional information
-
 lichenan 4.7 mg/ml, pH 6.5, 80°C, wild-type enzyme. 4.9 mg/ml, pH 6.5, 80°C, mutant enzyme delMet174 Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Pyrococcus furiosus O73951
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.39
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.39 barley beta-glucan + H2O main linkage type beta-1,3-1,4(Glc), about 10% of the specific activity compared to laminarin Pyrococcus furiosus ?
-
 ?
3.2.1.39 laminarin + H2O main linkage type beta-1,3-(Glc). The enzyme cleaves laminarin randomly, yielding glucose, laminaribiose, and higher laminari-oligosaccharides Pyrococcus furiosus ?
-
 ?
3.2.1.39 lichenan + H2O main linkage type beta-1,3-1,4(Glc), about 10% of the specific activity compared to laminarin Pyrococcus furiosus ?
-
 ?
3.2.1.39 additional information no activity with cellulose, xylan and chitin Pyrococcus furiosus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.39 endo-beta-1,3-glucanase
-
 Pyrococcus furiosus
3.2.1.39 LamA
-
 Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.39 80
-
 assay at Pyrococcus furiosus
3.2.1.39 100 105
-
 Pyrococcus furiosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.39 80 115 80°C: about 50% of maximal activity, 115°C: about 30% of maximal activity Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.39 80
-
 110 h, stable Pyrococcus furiosus
3.2.1.39 90
-
 half-life: 64 h Pyrococcus furiosus
3.2.1.39 100
-
 half-life: 19 h Pyrococcus furiosus
3.2.1.39 110
-
 half-life: 15 min Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.39 6 6.5
-
 Pyrococcus furiosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.39 4.5 8 pH 4.5: about 75% of maximal activity, pH 8.0: about 55% of maximal activity Pyrococcus furiosus