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Literature summary extracted from

  • Klump, H.; Di Ruggiero, J.; Kessel, M.; Park, J.B.; Adams, M.W.; Robb, F.T.
    Glutamate dehydrogenase from the hyperthermophile Pyrococcus furiosus. Thermal denaturation and activation (1992), J. Biol. Chem., 267, 22681-22685.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.3 270000
-
-
 Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.3 L-glutamate + H2O + NAD(P)+ Pyrococcus furiosus
-
 2-oxoglutarate + NH3 + NAD(P)H + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.3 Pyrococcus furiosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.3
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.3 L-glutamate + H2O + NAD(P)+
-
 Pyrococcus furiosus 2-oxoglutarate + NH3 + NAD(P)H + H+
-
 ?
1.4.1.3 L-glutamate + H2O + NAD+
-
 Pyrococcus furiosus 2-oxoglutarate + NH3 + NADH + H+
-
 ?
1.4.1.3 L-glutamate + H2O + NADP+
-
 Pyrococcus furiosus 2-oxoglutarate + NH3 + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.3 hexamer
-
 Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.3 85
-
 assay at Pyrococcus furiosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.4.1.3 40
-
 inactive at temperatures below 40°C, undergoes heat activation above 40°C Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.3 110
-
 thermal denaturation starts at 110°C and is completed at 118°C. The process of heat activation from 40 to 80°C is accompanied by a much smaller increase in absorbance at 280 nm and a reversible increase in heat capacity with DELTAcal = 187 Kcal/mol GDH and Tm = 57°C. This absorbance change as well as the moderate increase in heat capacity suggest that thermal activation leads to some exposure of hydrophobic groups to solvent water as the GDH structure is opened slightly. The increase in absorbance at 280 nm during activation is only 12% of that for denaturation Pyrococcus furiosus
1.4.1.3 113
-
 Tm-value = 113°C Pyrococcus furiosus
1.4.1.3 118
-
 thermal denaturation starts at 110°C and is comp1eted at 118°C Pyrococcus furiosus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.3 NAD+ both NAD+ and NADP+ are utilized as cofactors. The inactive enzyme also binds cofactors Pyrococcus furiosus
1.4.1.3 NADP+ both NAD+ and NADP+ are utilized as cofactors. The inactive enzyme also binds cofactors Pyrococcus furiosus