EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.4 | DNA and amino acid sequence determination and analysis, sequence comparison, subcloning in Escherichia coli strain JM109, expression in Escherichia coli strain BL21 (DE3) | Paenibacillus polymyxa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.4 | 0.2 | - |
(3R,3S)-acetoin | pH 7.0, 30°C | Paenibacillus polymyxa | |
1.1.1.4 | 0.32 | - |
NADH | pH 7.0, 30°C | Paenibacillus polymyxa | |
1.1.1.4 | 0.84 | - |
acetoin | (S)-acetoin, pH 7.0, 30°C | Paenibacillus polymyxa | |
1.1.1.4 | 2.3 | - |
NAD+ | pH 7.0, 30°C | Paenibacillus polymyxa | |
1.1.1.4 | 2.73 | - |
meso-butane-2,3-diol | pH 7.0, 30°C | Paenibacillus polymyxa | |
1.1.1.4 | 7.67 | - |
(2R,3R)-butane-2,3-diol | pH 7.0, 30°C | Paenibacillus polymyxa |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.4 | 44500 | - |
2 * 44500, SDS-PAGE | Paenibacillus polymyxa |
1.1.1.4 | 90000 | - |
gel filtration | Paenibacillus polymyxa |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.4 | Paenibacillus polymyxa | - |
isolated from soil | - |
1.1.1.4 | Paenibacillus polymyxa ZJ-9 | - |
isolated from soil | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.4 | native enzyme 35fold to homogeneity by fractional ammonium sulfate precipitation, followed by two different steps of anion exchange chromatography | Paenibacillus polymyxa |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.4 | cell culture | - |
Paenibacillus polymyxa | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.4 | 4.65 | - |
purified native enzyme, pH 7.0, 30°C | Paenibacillus polymyxa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.4 | (2R,3R)-butane-2,3-diol + NAD+ | - |
Paenibacillus polymyxa | (3R,3S)-acetoin + NADH + H+ | - |
r | |
1.1.1.4 | (2R,3R)-butane-2,3-diol + NAD+ | - |
Paenibacillus polymyxa ZJ-9 | (3R,3S)-acetoin + NADH + H+ | - |
r | |
1.1.1.4 | meso-butane-2,3-diol + NAD+ | - |
Paenibacillus polymyxa | acetoin + NADH + H+ | - |
r | |
1.1.1.4 | meso-butane-2,3-diol + NAD+ | - |
Paenibacillus polymyxa ZJ-9 | acetoin + NADH + H+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.4 | dimer | 2 * 44500, SDS-PAGE | Paenibacillus polymyxa |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.4 | 2,3-butanediol dehydrogenase | - |
Paenibacillus polymyxa |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.4 | 30 | - |
- |
Paenibacillus polymyxa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.4 | 6 | - |
oxidation reaction | Paenibacillus polymyxa |
1.1.1.4 | 8 | - |
reduction reaction | Paenibacillus polymyxa |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.4 | additional information | no activity with NADP+ or NADPH as the coenzyme, respectively | Paenibacillus polymyxa | |
1.1.1.4 | NAD+ | - |
Paenibacillus polymyxa | |
1.1.1.4 | NADH | - |
Paenibacillus polymyxa |