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Literature summary extracted from

  • Bauer, M.W.; Driskill, L.E.; Callen, W.; Snead, M.A.; Mathur, E.J.; Kelly, R.M.
    An endoglucanase, EglA, from the hyperthermophilic archaeon Pyrococcus furiosus hydrolyzes beta-1,4 bonds in mixed-linkage (1-->3),(1-->4)-beta-D-glucans and cellulose (1999), J. Bacteriol., 181, 284-290.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 expressed in Escherichia coli Pyrococcus furiosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 cellobiose
-
 Pyrococcus furiosus
3.2.1.4 D-glucono-1,5-lactone
-
 Pyrococcus furiosus
3.2.1.4 D-glucose
-
 Pyrococcus furiosus
3.2.1.4 laminaribiose
-
 Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 0.061
-
 4-nitrophenyl cellotrioside pH 6.0, 95°C Pyrococcus furiosus
3.2.1.4 0.12
-
 4-nitrophenyl cellotetraoside pH 6.0, 95°C Pyrococcus furiosus
3.2.1.4 0.15
-
 4-nitrophenyl cellobioside pH 6.0, 95°C Pyrococcus furiosus
3.2.1.4 0.36
-
 4-nitrophenyl cellopentaoside pH 6.0, 95°C Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 35900
-
 x * 35900, calculated from sequence Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Pyrococcus furiosus Q9V2T0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 4-nitrophenyl beta-D-glucoside + H2O low activity Pyrococcus furiosus 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.4 4-nitrophenyl cellobioside + H2O
-
 Pyrococcus furiosus 4-nitrophenol + cellobiose
-
 ?
3.2.1.4 4-nitrophenyl cellopentaoside + H2O
-
 Pyrococcus furiosus 4-nitrophenol + cellopentaose
-
 ?
3.2.1.4 4-nitrophenyl cellotetraoside + H2O
-
 Pyrococcus furiosus 4-nitrophenol + cellotetraose
-
 ?
3.2.1.4 4-nitrophenyl cellotrioside + H2O
-
 Pyrococcus furiosus 4-nitrophenol + cellotriose
-
 ?
3.2.1.4 barley beta-glucan + H2O the highest specific activity toward polysaccharides occurs with mixed-linkage (1->3),(1->4)-beta-D-glucans such as barley beta-glucan and lichenan Pyrococcus furiosus ?
-
 ?
3.2.1.4 cellohexaose + H2O
-
 Pyrococcus furiosus ?
-
 ?
3.2.1.4 cellopentaose + H2O
-
 Pyrococcus furiosus ?
-
 ?
3.2.1.4 lichenan + H2O the highest specific activity toward polysaccharides occurs with mixed-linkage (1->3),(1->4)-beta-D-glucans such as barley beta-D-glucan and lichenan Pyrococcus furiosus ?
-
 ?
3.2.1.4 additional information EglA hydrolyzes shorter cellodextrins (DP <5) as well as the amorphous portions of polysaccharides which contain only beta-1,4 bonds such as carboxymethyl cellulose, microcrystalline cellulose, Whatman paper, and cotton linter. Kinetics studies with cellooliogsaccharides and p-nitrophenyl-cellooligosaccharides indicated that the enzyme had three glucose binding subsites (-I, -II, and -III) for the nonreducing end and two glucose binding subsites (+I and +II) for the reducing end from the scissile glycosidic linkage. No activity is detected on solely beta-1,3-linked oligosaccharides or polysaccharides Pyrococcus furiosus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 35900, calculated from sequence Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 EglA
-
 Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 100
-
-
 Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 95
-
 half-life: 40 h Pyrococcus furiosus
3.2.1.4 112
-
 denaturing temperature Pyrococcus furiosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.4 0.79
-
 4-nitrophenyl cellobioside pH 6.0, 95°C Pyrococcus furiosus
3.2.1.4 1.4
-
 4-nitrophenyl cellopentaoside pH 6.0, 95°C Pyrococcus furiosus
3.2.1.4 3.6
-
 4-nitrophenyl cellotetraoside pH 6.0, 95°C Pyrococcus furiosus
3.2.1.4 8.3
-
 4-nitrophenyl cellotrioside pH 6.0, 95°C Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 6
-
-
 Pyrococcus furiosus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.4 0.1
-
 D-glucono-1,5-lactone pH 6.0, 95°C Pyrococcus furiosus
3.2.1.4 1.6
-
 laminaribiose pH 6.0, 95°C Pyrococcus furiosus
3.2.1.4 3
-
 cellobiose pH 6.0, 95°C Pyrococcus furiosus
3.2.1.4 90
-
 D-glucose pH 6.0, 95°C Pyrococcus furiosus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.4 0.06
-
 4-nitrophenyl beta-D-glucoside pH 6.0, 95°C Pyrococcus furiosus
3.2.1.4 6.5
-
 4-nitrophenyl cellopentaoside pH 6.0, 95°C Pyrococcus furiosus
3.2.1.4 8.7
-
 4-nitrophenyl cellobioside pH 6.0, 95°C Pyrococcus furiosus
3.2.1.4 49
-
 4-nitrophenyl cellotetraoside pH 6.0, 95°C Pyrococcus furiosus
3.2.1.4 220
-
 4-nitrophenyl cellotrioside pH 6.0, 95°C Pyrococcus furiosus