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Literature summary extracted from

  • Lendenmann, U.; Spain, J.C.
    2-Aminophenol 1,6-dioxygenase: a novel aromatic ring cleavage enzyme purified from Pseudomonas pseudoalcaligenes JS45 (1996), J. Bacteriol., 178, 6227-6232.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.13.11.74 dialysis against 50 mM MOPS (pH 7.3) containing ethanol (10% [v/v]) for 24 h abolishes 95% of the activity of 2-aminophenol 1,6-dioxygenase Pseudomonas oleovorans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.74 1,2,3-trihydroxybenzene complete inhibition at 0.05 mM Pseudomonas oleovorans
1.13.11.74 1,2,4-Trihydroxybenzene complete inhibition at 0.05 mM Pseudomonas oleovorans
1.13.11.74 2,2'-dipyridyl complete inhibition at 2 mM Pseudomonas oleovorans
1.13.11.74 2,3-Dihydroxybenzoic acid 20% inhibition at 0.05 mM Pseudomonas oleovorans
1.13.11.74 2-amino-p-cresol 90% inhibition at 0.05 mM Pseudomonas oleovorans
1.13.11.74 2-methoxyphenol 30% inhibition at 0.05 mM Pseudomonas oleovorans
1.13.11.74 3-Amino-4-hydroxybenzoic acid 20% inhibition at 0.05 mM Pseudomonas oleovorans
1.13.11.74 3-chlorocatechol complete inhibition at 0.05 mM Pseudomonas oleovorans
1.13.11.74 3-hydroxyanthranilic acid 20% inhibition at 0.05 mM Pseudomonas oleovorans
1.13.11.74 3-methylcatechol complete inhibition at 0.05 mM Pseudomonas oleovorans
1.13.11.74 4-amino-3-hydroxybenzoic acid 30% inhibition at 0.05 mM Pseudomonas oleovorans
1.13.11.74 4-amino-m-cresol 10% inhibition at 0.05 mM Pseudomonas oleovorans
1.13.11.74 4-aminoresorcinol complete inhibition at 0.05 mM Pseudomonas oleovorans
1.13.11.74 4-Chlorocatechol complete inhibition at 0.05 mM Pseudomonas oleovorans
1.13.11.74 4-methylcatechol complete inhibition at 0.05 mM Pseudomonas oleovorans
1.13.11.74 Ca2+ 84% residual activity at 2 mM Pseudomonas oleovorans
1.13.11.74 Cd2+ complete inhibition at 2 mM Pseudomonas oleovorans
1.13.11.74 Co2+ 47% residual activity at 2 mM Pseudomonas oleovorans
1.13.11.74 EDTA 70% residual activity at 2 mM Pseudomonas oleovorans
1.13.11.74 Fe3+ 78% residual activity at 2 mM Pseudomonas oleovorans
1.13.11.74 H2O2 complete inhibition at 1 mM Pseudomonas oleovorans
1.13.11.74 K3Fe(CN)6 17% residual activity at 1 mM Pseudomonas oleovorans
1.13.11.74 Mg2+ 85% residual activity at 2 mM Pseudomonas oleovorans
1.13.11.74 Mn2+ 5% residual activity at 2 mM Pseudomonas oleovorans
1.13.11.74 Mo2+ 96% residual activity at 2 mM Pseudomonas oleovorans
1.13.11.74 Ni2+ 50% residual activity at 2 mM Pseudomonas oleovorans
1.13.11.74 nitrilotriacetic acid 86% residual activity at 2 mM Pseudomonas oleovorans
1.13.11.74 o-phenanthroline 1% residual activity at 2 mM Pseudomonas oleovorans
1.13.11.74 protocatechuate 20% inhibition at 0.05 mM Pseudomonas oleovorans
1.13.11.74 Zn2+ complete inhibition at 2 mM Pseudomonas oleovorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.74 0.0042
-
 2-Aminophenol in 50 mM Tris (pH 8.0) at 25°C Pseudomonas oleovorans
1.13.11.74 0.71
-
 O2 in 50 mM Tris (pH 8.0) at 25°C Pseudomonas oleovorans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.74 Fe2+ ferrous iron is the sole cofactor, 100% activity at 2 mM Pseudomonas oleovorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.74 35000
-
 2 * 39000 + 2 * 35000, SDS-PAGE Pseudomonas oleovorans
1.13.11.74 39000
-
 2 * 39000 + 2 * 35000, SDS-PAGE Pseudomonas oleovorans
1.13.11.74 140000
-
 gel filtration Pseudomonas oleovorans

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.74 Pseudomonas oleovorans
-
-
-
1.13.11.74 Pseudomonas oleovorans JS45
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.74 ethanol precipitation, MonoQ column chromatography, and Sephacryl S-300 gel filtration Pseudomonas oleovorans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.74 1.5
-
 crude extract, in 50 mM Tris (pH 8.0) at 25°C Pseudomonas oleovorans
1.13.11.74 21.6
-
 purified enzyme, in 50 mM Tris (pH 8.0) at 25°C Pseudomonas oleovorans

Storage Stability

EC Number Storage Stability Organism
1.13.11.74 -20°C, purified enzyme in 50 mM MOPS at pH 7.3 in the presence of 10% (v/v) glycerol, several months, no loss of activity Pseudomonas oleovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.74 2-amino-4-chlorophenol + O2 15% activity compared to 2-aminophenol Pseudomonas oleovorans ?
-
 ?
1.13.11.74 2-amino-4-chlorophenol + O2 15% activity compared to 2-aminophenol Pseudomonas oleovorans JS45 ?
-
 ?
1.13.11.74 2-amino-m-cresol + O2 1% activity compared to 2-aminophenol Pseudomonas oleovorans ?
-
 ?
1.13.11.74 2-amino-m-cresol + O2 1% activity compared to 2-aminophenol Pseudomonas oleovorans JS45 ?
-
 ?
1.13.11.74 2-aminophenol + O2 100% activity Pseudomonas oleovorans 2-aminomuconate 6-semialdehyde
-
 ?
1.13.11.74 2-aminophenol + O2 100% activity Pseudomonas oleovorans JS45 2-aminomuconate 6-semialdehyde
-
 ?
1.13.11.74 6-amino-m-cresol + O2 25% activity compared to 2-aminophenol Pseudomonas oleovorans ?
-
 ?
1.13.11.74 catechol + O2 13% activity compared to 2-aminophenol Pseudomonas oleovorans ?
-
 ?
1.13.11.74 catechol + O2 13% activity compared to 2-aminophenol Pseudomonas oleovorans JS45 ?
-
 ?
1.13.11.74 additional information 3-methylcatechol, 4-methylcatechol, 3-chlorocatechol, 4-chlorocatechol, 1,2,3-trihydroxybenzene, 1,2,4-trihydroxybenzene, 4-aminoresorcinol, 2-amino-p-cresol, 2,3-dihydroxybenzoic acid, protocatechuate, 2-methoxyphenol, 4-amino-3-hydroxybenzoic acid, 3-amino-4-hydroxybenzoic acid, 3-hydroxyanthranilic acid, and 4-amino-m-cresol are no substrates Pseudomonas oleovorans ?
-
 ?
1.13.11.74 additional information 3-methylcatechol, 4-methylcatechol, 3-chlorocatechol, 4-chlorocatechol, 1,2,3-trihydroxybenzene, 1,2,4-trihydroxybenzene, 4-aminoresorcinol, 2-amino-p-cresol, 2,3-dihydroxybenzoic acid, protocatechuate, 2-methoxyphenol, 4-amino-3-hydroxybenzoic acid, 3-amino-4-hydroxybenzoic acid, 3-hydroxyanthranilic acid, and 4-amino-m-cresol are no substrates Pseudomonas oleovorans JS45 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.74 heterodimer 2 * 39000 + 2 * 35000, SDS-PAGE Pseudomonas oleovorans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.74 60
-
 the enzyme loses 50% of its activity upon incubation at 60°C for 5 min Pseudomonas oleovorans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.74 147
-
 2-Aminophenol in 50 mM Tris (pH 8.0) at 25°C Pseudomonas oleovorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.74 7 9
-
 Pseudomonas oleovorans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.74 5.5 10.5
-
 Pseudomonas oleovorans