EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.1.9 | synthesis | Gluconobacter oxydans strain NH-10 is useful for production of xylitol from D-arabitol via D-xylulose | Gluconobacter oxydans |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.9 | gene xdhA, coexpression of gene xdhA and cofactor regeneration enzyme, i.e. glucose dehydrogenase gene gdh from Bacillus subtilis, in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain JM109 | Gluconobacter oxydans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.9 | xylitol + NAD+ | Gluconobacter oxydans | - |
D-xylulose + NADH + H+ | - |
r | |
1.1.1.9 | xylitol + NAD+ | Gluconobacter oxydans NH-10 | - |
D-xylulose + NADH + H+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.9 | Gluconobacter oxydans | - |
isolated from soil, China | - |
1.1.1.9 | Gluconobacter oxydans NH-10 | - |
isolated from soil, China | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.9 | native enzyme 469fold from sstrain NH-10 by ammonium sulfate fractionation, two different steps of anion exchange chromatography, and gel filtration | Gluconobacter oxydans |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.9 | 0.76 | - |
purified native enzyme, pH 11.0, 30°C, substrate meso-erythritol | Gluconobacter oxydans |
1.1.1.9 | 6.74 | - |
purified native enzyme, pH 11.0, 30°C, substrate D-arabitol | Gluconobacter oxydans |
1.1.1.9 | 12.89 | - |
purified native enzyme, pH 11.0, 30°C, substrate D-mannitol | Gluconobacter oxydans |
1.1.1.9 | 102.5 | - |
purified native enzyme, pH 11.0, 30°C, substrate xylitol | Gluconobacter oxydans |
1.1.1.9 | 156.1 | - |
purified native enzyme, pH 11.0, 30°C, substrate D-sorbitol | Gluconobacter oxydans |
1.1.1.9 | 180.4 | - |
purified native enzyme, pH 5.0, 30°C, substrate D-fructose | Gluconobacter oxydans |
1.1.1.9 | 564 | - |
purified native enzyme, pH 5.0, 30°C, substrate D-xylulose | Gluconobacter oxydans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.9 | D-arabitol + NAD+ | - |
Gluconobacter oxydans | ? + NADH + H+ | - |
r | |
1.1.1.9 | D-arabitol + NAD+ | - |
Gluconobacter oxydans NH-10 | ? + NADH + H+ | - |
r | |
1.1.1.9 | D-mannitol + NAD+ | - |
Gluconobacter oxydans | D-fructose + NADH + H+ | - |
r | |
1.1.1.9 | D-mannitol + NAD+ | - |
Gluconobacter oxydans NH-10 | D-fructose + NADH + H+ | - |
r | |
1.1.1.9 | D-sorbitol + NAD+ | - |
Gluconobacter oxydans | L-sorbose + NADH + H+ | - |
r | |
1.1.1.9 | D-sorbitol + NAD+ | - |
Gluconobacter oxydans NH-10 | L-sorbose + NADH + H+ | - |
r | |
1.1.1.9 | D-xylulose + NAD+ | - |
Gluconobacter oxydans | xylitol + NADH + H+ | - |
r | |
1.1.1.9 | meso-erythritol + NAD+ | - |
Gluconobacter oxydans | ? + NADH + H+ | - |
r | |
1.1.1.9 | meso-erythritol + NAD+ | - |
Gluconobacter oxydans NH-10 | ? + NADH + H+ | - |
r | |
1.1.1.9 | xylitol + NAD+ | - |
Gluconobacter oxydans | D-xylulose + NADH + H+ | - |
r | |
1.1.1.9 | xylitol + NAD+ | - |
Gluconobacter oxydans NH-10 | D-xylulose + NADH + H+ | - |
r |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.9 | 30 | - |
reduction reaction | Gluconobacter oxydans |
1.1.1.9 | 35 | - |
oxidation reaction | Gluconobacter oxydans |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.9 | 20 | 55 | activity range, profile overview | Gluconobacter oxydans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.9 | 5.5 | - |
reduction reaction | Gluconobacter oxydans |
1.1.1.9 | 10.5 | 11 | oxidation reaction | Gluconobacter oxydans |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.9 | 4 | 8 | reduction reaction, activity range | Gluconobacter oxydans |
1.1.1.9 | 9 | 12 | oxidation reaction, activity range | Gluconobacter oxydans |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.9 | additional information | XDH depends exclusively on NAD+/NADH as cofactors with a relatively low activity | Gluconobacter oxydans | |
1.1.1.9 | NAD+ | - |
Gluconobacter oxydans | |
1.1.1.9 | NADH | - |
Gluconobacter oxydans |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.1.9 | metabolism | bioproduction pathway of xylitol, overview | Gluconobacter oxydans |
1.1.1.9 | additional information | XDH depends exclusively on NAD+/NADH as cofactors with a relatively low activity limiting the the overall conversion process, improvement by recombinant expression of enzyme and glucose dehydrogenase cofactor regeneration enzyme | Gluconobacter oxydans |