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Literature summary extracted from

  • Zhang, J.; Li, S.; Xu, H.; Zhou, P.; Zhang, L.; Ouyang, P.
    Purification of xylitol dehydrogenase and improved production of xylitol by increasing XDH activity and NADH supply in Gluconobacter oxydans (2013), J. Agric. Food Chem., 61, 2861-2867.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.1.1.9 synthesis Gluconobacter oxydans strain NH-10 is useful for production of xylitol from D-arabitol via D-xylulose Gluconobacter oxydans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.9 gene xdhA, coexpression of gene xdhA and cofactor regeneration enzyme, i.e. glucose dehydrogenase gene gdh from Bacillus subtilis, in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain JM109 Gluconobacter oxydans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.9 xylitol + NAD+ Gluconobacter oxydans
-
D-xylulose + NADH + H+
-
r
1.1.1.9 xylitol + NAD+ Gluconobacter oxydans NH-10
-
D-xylulose + NADH + H+
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.9 Gluconobacter oxydans
-
isolated from soil, China
-
1.1.1.9 Gluconobacter oxydans NH-10
-
isolated from soil, China
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.9 native enzyme 469fold from sstrain NH-10 by ammonium sulfate fractionation, two different steps of anion exchange chromatography, and gel filtration Gluconobacter oxydans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.9 0.76
-
purified native enzyme, pH 11.0, 30°C, substrate meso-erythritol Gluconobacter oxydans
1.1.1.9 6.74
-
purified native enzyme, pH 11.0, 30°C, substrate D-arabitol Gluconobacter oxydans
1.1.1.9 12.89
-
purified native enzyme, pH 11.0, 30°C, substrate D-mannitol Gluconobacter oxydans
1.1.1.9 102.5
-
purified native enzyme, pH 11.0, 30°C, substrate xylitol Gluconobacter oxydans
1.1.1.9 156.1
-
purified native enzyme, pH 11.0, 30°C, substrate D-sorbitol Gluconobacter oxydans
1.1.1.9 180.4
-
purified native enzyme, pH 5.0, 30°C, substrate D-fructose Gluconobacter oxydans
1.1.1.9 564
-
purified native enzyme, pH 5.0, 30°C, substrate D-xylulose Gluconobacter oxydans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.9 D-arabitol + NAD+
-
Gluconobacter oxydans ? + NADH + H+
-
r
1.1.1.9 D-arabitol + NAD+
-
Gluconobacter oxydans NH-10 ? + NADH + H+
-
r
1.1.1.9 D-mannitol + NAD+
-
Gluconobacter oxydans D-fructose + NADH + H+
-
r
1.1.1.9 D-mannitol + NAD+
-
Gluconobacter oxydans NH-10 D-fructose + NADH + H+
-
r
1.1.1.9 D-sorbitol + NAD+
-
Gluconobacter oxydans L-sorbose + NADH + H+
-
r
1.1.1.9 D-sorbitol + NAD+
-
Gluconobacter oxydans NH-10 L-sorbose + NADH + H+
-
r
1.1.1.9 D-xylulose + NAD+
-
Gluconobacter oxydans xylitol + NADH + H+
-
r
1.1.1.9 meso-erythritol + NAD+
-
Gluconobacter oxydans ? + NADH + H+
-
r
1.1.1.9 meso-erythritol + NAD+
-
Gluconobacter oxydans NH-10 ? + NADH + H+
-
r
1.1.1.9 xylitol + NAD+
-
Gluconobacter oxydans D-xylulose + NADH + H+
-
r
1.1.1.9 xylitol + NAD+
-
Gluconobacter oxydans NH-10 D-xylulose + NADH + H+
-
r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.9 30
-
reduction reaction Gluconobacter oxydans
1.1.1.9 35
-
oxidation reaction Gluconobacter oxydans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.9 20 55 activity range, profile overview Gluconobacter oxydans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.9 5.5
-
reduction reaction Gluconobacter oxydans
1.1.1.9 10.5 11 oxidation reaction Gluconobacter oxydans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.9 4 8 reduction reaction, activity range Gluconobacter oxydans
1.1.1.9 9 12 oxidation reaction, activity range Gluconobacter oxydans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.9 additional information XDH depends exclusively on NAD+/NADH as cofactors with a relatively low activity Gluconobacter oxydans
1.1.1.9 NAD+
-
Gluconobacter oxydans
1.1.1.9 NADH
-
Gluconobacter oxydans

General Information

EC Number General Information Comment Organism
1.1.1.9 metabolism bioproduction pathway of xylitol, overview Gluconobacter oxydans
1.1.1.9 additional information XDH depends exclusively on NAD+/NADH as cofactors with a relatively low activity limiting the the overall conversion process, improvement by recombinant expression of enzyme and glucose dehydrogenase cofactor regeneration enzyme Gluconobacter oxydans