Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yeom, S.J.; Kim, B.N.; Kim, Y.S.; Oh, D.K.
    Hydrolysis of isoflavone glycosides by a thermostable beta-glucosidase from Pyrococcus furiosus (2012), J. Agric. Food Chem., 60, 1535-1541.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.B28 additional information not activated by monovalent or divalent cations Pyrococcus furiosus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.B28 expression in Escherichia coli Pyrococcus furiosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.B28 additional information not inhibited by EDTA Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.B28 0.33
-
 glycitin pH 6.0, 95°C Pyrococcus furiosus
3.2.1.B28 0.42
-
 daidzin pH 6.0, 95°C Pyrococcus furiosus
3.2.1.B28 0.5
-
 genistin pH 6.0, 95°C Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.B28 56000
-
 4 * 56000, SDS-PAGE Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.B28 Pyrococcus furiosus Q51723
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.B28
-
 Pyrococcus furiosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.B28 75
-
 substrate: glycitin, pH 6.0, 95°C Pyrococcus furiosus
3.2.1.B28 180
-
 substrate: daidzin, pH 6.0, 95°C Pyrococcus furiosus
3.2.1.B28 330
-
 substrate: genistin, pH 6.0, 95°C Pyrococcus furiosus
3.2.1.B28 912
-
 substrate: 4-nitrophenyl-beta-D-glucopyranoside, pH 6.0, 95°C Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.B28 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Pyrococcus furiosus 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.B28 daidzin + H2O
-
 Pyrococcus furiosus beta-D-glucose + daidzein
-
 ?
3.2.1.B28 genistin + H2O
-
 Pyrococcus furiosus beta-D-glucose + genistein
-
 ?
3.2.1.B28 glycitin + H2O i.e. glycitein 7-O-beta-glucoside Pyrococcus furiosus beta-D-glucose + glycitein
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.B28 tetramer 4 * 56000, SDS-PAGE Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.B28 95
-
-
 Pyrococcus furiosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.B28 75 98 75°C: about 50% of maximal activity, 98°C: about 85% of maximal activity Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.B28 70
-
 half-life: 1020 h Pyrococcus furiosus
3.2.1.B28 75
-
 half-life: 476 h Pyrococcus furiosus
3.2.1.B28 80
-
 half-life: 307 h Pyrococcus furiosus
3.2.1.B28 85
-
 half-life: 181 h Pyrococcus furiosus
3.2.1.B28 90
-
 half-life: 96 h Pyrococcus furiosus
3.2.1.B28 95
-
 half-life: 59 h Pyrococcus furiosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.B28 610
-
 glycitin pH 6.0, 95°C Pyrococcus furiosus
3.2.1.B28 1880
-
 daidzin pH 6.0, 95°C Pyrococcus furiosus
3.2.1.B28 6050
-
 genistin pH 6.0, 95°C Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.B28 6
-
-
 Pyrococcus furiosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.B28 5 7 pH 5.0: 80% of maximal activity, pH 8.0: about 80% of maximal activity Pyrococcus furiosus