Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Abken, H.-J.; Deppenmeier, U.
    Purification and properties of an F420H2 dehydrogenase from Methanosarcina mazei Gö1 (1997), FEMS Microbiol. Lett., 154, 231-237.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.98.4 0.007
-
 reduced coenzyme F420 pH 8.5, 39°C Methanosarcina mazei Gö1

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.98.4 membrane
-
 Methanosarcina mazei Gö1 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.98.4 Iron-sulfur cluster the enzyme contains 7 mol S2- and 7 mol Fe Methanosarcina mazei Gö1

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.98.4 16000
-
 x * 40000 + x * 37000 + x * 22000 + x * 20000 x * 16000, SDS-PAGE Methanosarcina mazei Gö1
1.1.98.4 20000
-
 x * 40000 + x * 37000 + x * 22000 + x * 20000 x * 16000, SDS-PAGE Methanosarcina mazei Gö1
1.1.98.4 22000
-
 x * 40000 + x * 37000 + x * 22000 + x * 20000 x * 16000, SDS-PAGE Methanosarcina mazei Gö1
1.1.98.4 37000
-
 x * 40000 + x * 37000 + x * 22000 + x * 20000 x * 16000, SDS-PAGE Methanosarcina mazei Gö1
1.1.98.4 40000
-
 x * 40000 + x * 37000 + x * 22000 + x * 20000 x * 16000, SDS-PAGE Methanosarcina mazei Gö1
1.1.98.4 115000
-
 gel filtration Methanosarcina mazei Gö1

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.98.4 additional information Methanosarcina mazei Gö1 the enzyme is involved in the reduction of heterodisulfide ?
-
 ?
1.1.98.4 additional information Methanosarcina mazei Gö1 DSM 3647 the enzyme is involved in the reduction of heterodisulfide ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.98.4 Methanosarcina mazei Gö1
-
-
-
1.1.98.4 Methanosarcina mazei Gö1 DSM 3647
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.98.4
-
 Methanosarcina mazei Gö1

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.98.4 17
-
 activity with reduced coenzyme F420 as electron donor and methylviologen and metronidazole as electron acceptors at pH 8.5 at a temperature of 39°C Methanosarcina mazei Gö1
1.1.98.4 68
-
 activity with reduced coenzyme F420 as electron donor and tetramethyl-4-benzoquinone as electron acceptors at pH 8.5 at a temperature of 39°C Methanosarcina mazei Gö1
1.1.98.4 136
-
 activity with reduced coenzyme F420 as electron donor and 2,3-dimethyl-1,4-naphthoquinone as electron acceptors at pH 8.5 at a temperature of 39°C Methanosarcina mazei Gö1
1.1.98.4 173
-
 activity with reduced coenzyme F420 as electron donor and 2-methyl-1,4-naphthoquinone as electron acceptors at pH 8.5 at a temperature of 39°C Methanosarcina mazei Gö1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.98.4 2,3-dimethyl-1,4-naphthoquinone + reduced coenzyme F420
-
 Methanosarcina mazei Gö1 2,3-dimethyl-1,4-naphthoquinol + oxidized coenzyme F420
-
 ?
1.1.98.4 2,3-dimethyl-1,4-naphthoquinone + reduced coenzyme F420
-
 Methanosarcina mazei Gö1 DSM 3647 2,3-dimethyl-1,4-naphthoquinol + oxidized coenzyme F420
-
 ?
1.1.98.4 2-methyl-1,4-naphthoquinone + reduced coenzyme F420 i.e. menadione Methanosarcina mazei Gö1 2-methyl-1,4-naphthoquinol + oxidized coenzyme F420
-
 ?
1.1.98.4 2-methyl-1,4-naphthoquinone + reduced coenzyme F420 i.e. menadione Methanosarcina mazei Gö1 DSM 3647 2-methyl-1,4-naphthoquinol + oxidized coenzyme F420
-
 ?
1.1.98.4 additional information the enzyme is involved in the reduction of heterodisulfide Methanosarcina mazei Gö1 ?
-
 ?
1.1.98.4 additional information no hydrogenase activity. No activity with NAD+ or NADP+ Methanosarcina mazei Gö1 ?
-
 ?
1.1.98.4 additional information the enzyme is involved in the reduction of heterodisulfide Methanosarcina mazei Gö1 DSM 3647 ?
-
 ?
1.1.98.4 additional information no hydrogenase activity. No activity with NAD+ or NADP+ Methanosarcina mazei Gö1 DSM 3647 ?
-
 ?
1.1.98.4 tetramethyl-4-benzoquinone + reduced coenzyme F420 i.e. menadione Methanosarcina mazei Gö1 tetramethyl-1,4-benzoquinol + oxidized coenzyme F420
-
 ?
1.1.98.4 tetramethyl-4-benzoquinone + reduced coenzyme F420 i.e. menadione Methanosarcina mazei Gö1 DSM 3647 tetramethyl-1,4-benzoquinol + oxidized coenzyme F420
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.98.4 oligomer x * 40000 + x * 37000 + x * 22000 + x * 20000 x * 16000, SDS-PAGE Methanosarcina mazei Gö1

Synonyms

EC Number Synonyms Comment Organism
1.1.98.4 F420H2 dehydrogenase part of the energy-conserving F420H2:heterodisulfide oxidoreductase system (EC 1.8.98.1) Methanosarcina mazei Gö1

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.98.4 FAD contains 0.2 mol FAD per mol of enzyme Methanosarcina mazei Gö1

General Information

EC Number General Information Comment Organism
1.1.98.4 physiological function the enzyme is involved in the reduction of heterodisulfide Methanosarcina mazei Gö1