Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Son, l.e..T.; Ko, K.M.; Cho, J.H.; Singaravelu, G.; Chatterjee, I.; Choi, T.W.; Song, H.O.; Yu, J.R.; Park, B.J.; Lee, S.K.; Ahnn, J.
    DHS-21, a dicarbonyl/L-xylulose reductase (DCXR) ortholog, regulates longevity and reproduction in Caenorhabditis elegans (2011), FEBS Lett., 585, 1310-1316.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.10 gene dhs-21, DNA and amino acid sequence determination and analysis, the dhs-21 gene is physically mapped to the cosmid R11D1 between the sma-1 and mes-4genes on chromosome V, expression of His6-tagged enzyme. Spatial and temporal expression pattern of the DHS-21-GFP construct in transgenic worms, overview Caenorhabditis elegans

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.10 additional information library screening and isolation of a dhs-21 deletion mutant Caenorhabditis elegans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.10 0.17
-
 L-erythrulose pH 7.0, temperature not specified in the publication Caenorhabditis elegans
1.1.1.10 0.18
-
 L-ribulose pH 7.0, temperature not specified in the publication Caenorhabditis elegans
1.1.1.10 0.27
-
 D-erythrose pH 7.0, temperature not specified in the publication Caenorhabditis elegans
1.1.1.10 0.66
-
 L-xylulose pH 7.0, temperature not specified in the publication Caenorhabditis elegans
1.1.1.10 0.68
-
 D-threose pH 7.0, temperature not specified in the publication Caenorhabditis elegans
1.1.1.10 2.58
-
 D-xylulose pH 7.0, temperature not specified in the publication Caenorhabditis elegans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.10 plasma membrane of spermatids Caenorhabditis elegans 5886
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.10 L-xylulose + NADPH + H+ Caenorhabditis elegans
-
 xylitol + NADP+
-
 ir
1.1.1.10 additional information Caenorhabditis elegans dicarbonyl/L-xylulose reductase is bifunctional ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.10 Caenorhabditis elegans Q21929 gene dhs-21
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.10 gonadal sheath cell
-
 Caenorhabditis elegans
-
1.1.1.10 intestine
-
 Caenorhabditis elegans
-
1.1.1.10 spermathecal-uterine valve
-
 Caenorhabditis elegans
-
1.1.1.10 spermatid
-
 Caenorhabditis elegans
-
1.1.1.10 uterine seam cell
-
 Caenorhabditis elegans
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.10 D-erythrose + NADPH + H+
-
 Caenorhabditis elegans ? + NADP+
-
 ir
1.1.1.10 D-threose + NADPH + H+
-
 Caenorhabditis elegans ? + NADP+
-
 ir
1.1.1.10 D-xylulose + NADPH + H+
-
 Caenorhabditis elegans xylitol + NADP+
-
 ?
1.1.1.10 L-erythrulose + NADPH + H+
-
 Caenorhabditis elegans ? + NADP+
-
 ir
1.1.1.10 L-ribulose + NADPH + H+ low activity Caenorhabditis elegans ? + NADP+
-
 ?
1.1.1.10 L-xylulose + NADPH + H+
-
 Caenorhabditis elegans xylitol + NADP+
-
 ir
1.1.1.10 additional information substrate specificity, overview Caenorhabditis elegans ?
-
 ?
1.1.1.10 additional information dicarbonyl/L-xylulose reductase is bifunctional Caenorhabditis elegans ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.10 DCXR
-
 Caenorhabditis elegans
1.1.1.10 dicarbonyl/L-xylulose reductase
-
 Caenorhabditis elegans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.10 0.49
-
 L-ribulose pH 7.0, temperature not specified in the publication Caenorhabditis elegans
1.1.1.10 1.22
-
 D-xylulose pH 7.0, temperature not specified in the publication Caenorhabditis elegans
1.1.1.10 1.8
-
 D-erythrose pH 7.0, temperature not specified in the publication Caenorhabditis elegans
1.1.1.10 2.32
-
 D-threose pH 7.0, temperature not specified in the publication Caenorhabditis elegans
1.1.1.10 3.32
-
 L-erythrulose pH 7.0, temperature not specified in the publication Caenorhabditis elegans
1.1.1.10 4.98
-
 L-xylulose pH 7.0, temperature not specified in the publication Caenorhabditis elegans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.10 7
-
 assay at Caenorhabditis elegans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.10 additional information no activity with NADH Caenorhabditis elegans
1.1.1.10 NADPH
-
 Caenorhabditis elegans

Expression

EC Number Organism Comment Expression
1.1.1.10 Caenorhabditis elegans knock-down of daf-16 and elt-2 transcription factors affects dhs-21 expression down

General Information

EC Number General Information Comment Organism
1.1.1.10 malfunction dhs-21 null mutants show defects in longevity, reproduction and egg-laying Caenorhabditis elegans
1.1.1.10 metabolism dicarbonyl/L-xylulose reductase converts L-xylulose into xylitol, and reduces various alpha-dicarbonyl compounds, performing a dual role in carbohydrate metabolism and detoxification Caenorhabditis elegans
1.1.1.10 physiological function the enzyme is essential for normal life span and reproduction Caenorhabditis elegans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.10 0.36
-
 L-ribulose pH 7.0, temperature not specified in the publication Caenorhabditis elegans
1.1.1.10 0.47
-
 D-xylulose pH 7.0, temperature not specified in the publication Caenorhabditis elegans
1.1.1.10 3.42
-
 D-threose pH 7.0, temperature not specified in the publication Caenorhabditis elegans
1.1.1.10 6.75
-
 D-erythrose pH 7.0, temperature not specified in the publication Caenorhabditis elegans
1.1.1.10 7.55
-
 L-xylulose pH 7.0, temperature not specified in the publication Caenorhabditis elegans
1.1.1.10 19.03
-
 L-erythrulose pH 7.0, temperature not specified in the publication Caenorhabditis elegans