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Literature summary extracted from

  • Inoue, K.; Ebizuka, Y.
    Purification and characterization of furostanol glycoside 26-O-beta-glucosidase from Costus speciosus rhizomes (1996), FEBS Lett., 378, 157-160.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.186 beta-sitosterol
-
 Hellenia speciosa
3.2.1.186 cholesterol
-
 Hellenia speciosa
3.2.1.186 conduritol beta-epoxide
-
 Hellenia speciosa
3.2.1.186 D-glucono-1,5-lactone 0.25 mM, 50% inhibition Hellenia speciosa
3.2.1.186 diosgenin 0.079, 50% inhibition Hellenia speciosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.186 0.05
-
 protogracillin pH 5.0, 40°C Hellenia speciosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.186 54000
-
 1 * 54000 + 1 * 58000, SDS-PAGE Hellenia speciosa
3.2.1.186 58000
-
 1 * 54000 + 1 * 58000, SDS-PAGE Hellenia speciosa
3.2.1.186 110000
-
 gel filtration Hellenia speciosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.186 additional information Hellenia speciosa the enzyme is involved in the postharvest conversion of furostanol glycosides to spirostanol glycosides in Costus speciosus. Furostanol glycosides are the precursors of spirostane glycosides, containing a glucose unit attached to the C-26 hydroxyl of the aglycone ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.186 Hellenia speciosa Q42707
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.186
-
 Hellenia speciosa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.186 rhizome
-
 Hellenia speciosa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.186 325
-
 pH 5.0, 40°C Hellenia speciosa

Storage Stability

EC Number Storage Stability Organism
3.2.1.186 4°C, 20 mM potassium phosphate buffer, pH 7.0, stable for 3 weeks Hellenia speciosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.186 4-nitrophenyl beta-D-glucoside + H2O
-
 Hellenia speciosa 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.186 additional information the enzyme is involved in the postharvest conversion of furostanol glycosides to spirostanol glycosides in Costus speciosus. Furostanol glycosides are the precursors of spirostane glycosides, containing a glucose unit attached to the C-26 hydroxyl of the aglycone Hellenia speciosa ?
-
 ?
3.2.1.186 additional information no activity amygdalin, cellobiose, gentiobiose, octyl beta-D-glucoside or prunasin Hellenia speciosa ?
-
 ?
3.2.1.186 protodioscin + H2O
-
 Hellenia speciosa 26-deglucoprotodioscin + D-glucose
-
 ?
3.2.1.186 protogracillin the enzyme is highly specific for cleavage of the C26-bound glucose moiety of furostanol glycosides Hellenia speciosa 26-deglucoprotogracillin + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.186 dimer 1 * 54000 + 1 * 58000, SDS-PAGE Hellenia speciosa

Synonyms

EC Number Synonyms Comment Organism
3.2.1.186 F26G
-
 Hellenia speciosa
3.2.1.186 furostanol 26-O-beta-D-glucoside glucohydrolase
-
 Hellenia speciosa
3.2.1.186 furostanol glycoside 26-O-beta-D-glucosidase
-
 Hellenia speciosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.186 40
-
 assay at Hellenia speciosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.186 5
-
 assay at Hellenia speciosa
3.2.1.186 5 5.5 substrate: protogracillin Hellenia speciosa

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.2.1.186 1.4
-
 pH 5.0, 40°C Hellenia speciosa beta-sitosterol
3.2.1.186 2.4
-
 pH 5.0, 40°C Hellenia speciosa conduritol beta-epoxide
3.2.1.186 2.8
-
 pH 5.0, 40°C Hellenia speciosa cholesterol