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Literature summary extracted from

  • Mutahir, Z.; Clausen, A.R.; Andersson, K.M.; Wisen, S.M.; Munch-Petersen, B.; Piskur, J.
    Thymidine kinase 1 regulatory fine-tuning through tetramer formation (2013), FEBS J., 280, 1531-1541.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.21 expressed in Escherichia coli BL21(DE3) cells Homo sapiens
2.7.1.21 expressed in Escherichia coli BL21(DE3) cells Dictyostelium discoideum
2.7.1.21 expressed in Escherichia coli BL21(DE3) cells Gallus gallus
2.7.1.21 expressed in Escherichia coli BL21(DE3) cells Rattus norvegicus
2.7.1.21 expressed in Escherichia coli BL21(DE3) cells Bacillus cereus
2.7.1.21 expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana
2.7.1.21 expressed in Escherichia coli BL21(DE3) cells Caenorhabditis elegans
2.7.1.21 expressed in Escherichia coli BL21(DE3) cells Danio rerio
2.7.1.21 expressed in Escherichia coli BL21(DE3) cells Xenopus tropicalis
2.7.1.21 expressed in Escherichia coli BL21(DE3) cells Anolis carolinensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.21 0.0003
-
 thymidine in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Rattus norvegicus
2.7.1.21 0.0005
-
 thymidine in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Homo sapiens
2.7.1.21 0.0006
-
 thymidine in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Danio rerio
2.7.1.21 0.0006
-
 thymidine in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Bacillus cereus
2.7.1.21 0.0006
-
 thymidine in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Caenorhabditis elegans
2.7.1.21 0.0006
-
 thymidine subunit alpha, in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Arabidopsis thaliana
2.7.1.21 0.0007
-
 thymidine in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Bacillus cereus
2.7.1.21 0.0007
-
 thymidine subunit alpha, in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Arabidopsis thaliana
2.7.1.21 0.0009
-
 thymidine in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Caenorhabditis elegans
2.7.1.21 0.0009
-
 thymidine subunit beta, in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Arabidopsis thaliana
2.7.1.21 0.0009
-
 thymidine subunit beta, in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Arabidopsis thaliana
2.7.1.21 0.0012
-
 thymidine in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Gallus gallus
2.7.1.21 0.0014
-
 thymidine in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Danio rerio
2.7.1.21 0.0016
-
 thymidine in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Xenopus tropicalis
2.7.1.21 0.002
-
 thymidine in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Anolis carolinensis
2.7.1.21 0.0024
-
 thymidine in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Dictyostelium discoideum
2.7.1.21 0.0025
-
 thymidine in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Anolis carolinensis
2.7.1.21 0.008
-
 thymidine in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Dictyostelium discoideum
2.7.1.21 0.012
-
 thymidine in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Gallus gallus
2.7.1.21 0.016
-
 thymidine in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Homo sapiens
2.7.1.21 0.017
-
 thymidine in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Rattus norvegicus
2.7.1.21 0.017
-
 thymidine in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication Xenopus tropicalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.21 21700
-
 2 * 21700, calculated from amino acid sequence Bacillus cereus
2.7.1.21 24800
-
 4 * 24800, calculated from amino acid sequence Gallus gallus
2.7.1.21 25400
-
 2 * 25400, calculated from amino acid sequence Dictyostelium discoideum
2.7.1.21 25400
-
 4 * 25400, calculated from amino acid sequence Homo sapiens
2.7.1.21 25600
-
 4 * 25600, calculated from amino acid sequence Danio rerio
2.7.1.21 25600
-
 4 * 25600, calculated from amino acid sequence Anolis carolinensis
2.7.1.21 25700
-
 4 * 25700, calculated from amino acid sequence Rattus norvegicus
2.7.1.21 26000
-
 2 * 26000 + 2 * 30700, calculated from amino acid sequence Arabidopsis thaliana
2.7.1.21 26300
-
 4 * 26300, calculated from amino acid sequence Xenopus tropicalis
2.7.1.21 29700
-
 4 * 29700, calculated from amino acid sequence Caenorhabditis elegans
2.7.1.21 30700
-
 2 * 26000 + 2 * 30700, calculated from amino acid sequence Arabidopsis thaliana
2.7.1.21 32000
-
 for subunit alpha pre-incubated without ATP, two different peaks at 52000 and 32000 Da are obtained during gel filtration Arabidopsis thaliana
2.7.1.21 43000
-
 gel filtration Bacillus cereus
2.7.1.21 46000
-
 gel filtration Gallus gallus
2.7.1.21 49000
-
 subunit beta, gel filtration Arabidopsis thaliana
2.7.1.21 52000
-
 for subunit alpha pre-incubated without ATP, two different peaks at 52000 and 32000 Da are obtained during gel filtration Arabidopsis thaliana
2.7.1.21 55000
-
 gel filtration Rattus norvegicus
2.7.1.21 57000
-
 gel filtration Dictyostelium discoideum
2.7.1.21 57000
-
 gel filtration Anolis carolinensis
2.7.1.21 58000
-
 gel filtration Homo sapiens
2.7.1.21 64000
-
 for enzyme pre-incubated without ATP, two different peaks at 98000 and 64000 Da are obtained during gel filtration Xenopus tropicalis
2.7.1.21 82000
-
 gel filtration Danio rerio
2.7.1.21 98000
-
 for enzyme pre-incubated without ATP, two different peaks at 98000 and 64000 Da are obtained during gel filtration Xenopus tropicalis
2.7.1.21 130000
-
 gel filtration Caenorhabditis elegans

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.21 Anolis carolinensis C3VAL3
-
-
2.7.1.21 Arabidopsis thaliana Q9S750 and Q9FN47 subunits alpha and beta
-
2.7.1.21 Bacillus cereus Q0H0H6
-
-
2.7.1.21 Caenorhabditis elegans G5EEZ5
-
-
2.7.1.21 Danio rerio Q803Y1
-
-
2.7.1.21 Dictyostelium discoideum Q27564
-
-
2.7.1.21 Gallus gallus P04047
-
-
2.7.1.21 Homo sapiens P04183
-
-
2.7.1.21 Rattus norvegicus P27158
-
-
2.7.1.21 Xenopus tropicalis Q5I0A2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.21 ATP + thymidine
-
 Homo sapiens ADP + dTMP
-
 ?
2.7.1.21 ATP + thymidine
-
 Dictyostelium discoideum ADP + dTMP
-
 ?
2.7.1.21 ATP + thymidine
-
 Gallus gallus ADP + dTMP
-
 ?
2.7.1.21 ATP + thymidine
-
 Rattus norvegicus ADP + dTMP
-
 ?
2.7.1.21 ATP + thymidine
-
 Bacillus cereus ADP + dTMP
-
 ?
2.7.1.21 ATP + thymidine
-
 Arabidopsis thaliana ADP + dTMP
-
 ?
2.7.1.21 ATP + thymidine
-
 Caenorhabditis elegans ADP + dTMP
-
 ?
2.7.1.21 ATP + thymidine
-
 Danio rerio ADP + dTMP
-
 ?
2.7.1.21 ATP + thymidine
-
 Xenopus tropicalis ADP + dTMP
-
 ?
2.7.1.21 ATP + thymidine
-
 Anolis carolinensis ADP + dTMP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.21 heterotetramer 2 * 26000 + 2 * 30700, calculated from amino acid sequence Arabidopsis thaliana
2.7.1.21 homodimer 2 * 21700, calculated from amino acid sequence Bacillus cereus
2.7.1.21 homodimer 2 * 25400, calculated from amino acid sequence Dictyostelium discoideum
2.7.1.21 homotetramer 4 * 24800, calculated from amino acid sequence Gallus gallus
2.7.1.21 homotetramer 4 * 25400, calculated from amino acid sequence Homo sapiens
2.7.1.21 homotetramer 4 * 25600, calculated from amino acid sequence Danio rerio
2.7.1.21 homotetramer 4 * 25600, calculated from amino acid sequence Anolis carolinensis
2.7.1.21 homotetramer 4 * 25700, calculated from amino acid sequence Rattus norvegicus
2.7.1.21 homotetramer 4 * 26300, calculated from amino acid sequence Xenopus tropicalis
2.7.1.21 homotetramer 4 * 29700, calculated from amino acid sequence Caenorhabditis elegans

Synonyms

EC Number Synonyms Comment Organism
2.7.1.21 thymidine kinase 1
-
 Homo sapiens
2.7.1.21 thymidine kinase 1
-
 Dictyostelium discoideum
2.7.1.21 thymidine kinase 1
-
 Gallus gallus
2.7.1.21 thymidine kinase 1
-
 Rattus norvegicus
2.7.1.21 thymidine kinase 1
-
 Bacillus cereus
2.7.1.21 thymidine kinase 1
-
 Arabidopsis thaliana
2.7.1.21 thymidine kinase 1
-
 Caenorhabditis elegans
2.7.1.21 thymidine kinase 1
-
 Danio rerio
2.7.1.21 thymidine kinase 1
-
 Xenopus tropicalis
2.7.1.21 thymidine kinase 1
-
 Anolis carolinensis
2.7.1.21 TK1
-
 Homo sapiens
2.7.1.21 TK1
-
 Dictyostelium discoideum
2.7.1.21 TK1
-
 Gallus gallus
2.7.1.21 TK1
-
 Rattus norvegicus
2.7.1.21 TK1
-
 Bacillus cereus
2.7.1.21 TK1
-
 Arabidopsis thaliana
2.7.1.21 TK1
-
 Caenorhabditis elegans
2.7.1.21 TK1
-
 Danio rerio
2.7.1.21 TK1
-
 Xenopus tropicalis
2.7.1.21 TK1
-
 Anolis carolinensis