EC Number | Cloned (Comment) | Organism |
---|---|---|
2.6.1.1 | expressed in Escherichia coli as a His-tagged fusion protein | Bacillus subtilis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.6.1.1 | D232N | mutant shows no activity | Bacillus subtilis |
2.6.1.1 | K270H | mutant shows no activity | Bacillus subtilis |
2.6.1.1 | R403Y | mutant shows no activity | Bacillus subtilis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.1 | 0.3 | - |
2-oxoglutarate | pH 8.0, 45°C | Bacillus subtilis | |
2.6.1.1 | 0.6 | - |
oxaloacetate | pH 8.0, 45°C | Bacillus subtilis | |
2.6.1.1 | 6.7 | - |
L-aspartate | pH 8.0, 45°C | Bacillus subtilis | |
2.6.1.1 | 8 | - |
L-glutamate | pH 8.0, 45°C | Bacillus subtilis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.1 | CaCl2 | 1 mM relative activity: 115.5% | Bacillus subtilis | |
2.6.1.1 | CoCl2 | 1 mM relative activity: 122.1% | Bacillus subtilis | |
2.6.1.1 | CuSO4 | 1 mM relative activity: 84.3% | Bacillus subtilis | |
2.6.1.1 | EDTA | 1 mM relative activity: 106.4% | Bacillus subtilis | |
2.6.1.1 | MgCl2 | 1 mM relative activity: 96.9% | Bacillus subtilis | |
2.6.1.1 | MnSO4 | 1 mM relative activity: 90.1% | Bacillus subtilis | |
2.6.1.1 | ZnSO4 | 1 mM relative activity: 99.7% | Bacillus subtilis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.6.1.1 | 49100 | - |
calculated from cDNA | Bacillus subtilis |
2.6.1.1 | 55000 | - |
SDS-PAGE | Bacillus subtilis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.6.1.1 | Bacillus subtilis | Q939C9 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.1 | L-aspartate + 2-oxoglutarate | relative activity L-aspartate: 100% | Bacillus subtilis | oxaloacetate + L-glutamate | - |
? | |
2.6.1.1 | L-aspartate + oxaloacetate | relative activity compared to 2-oxoglutarate: 81.5% | Bacillus subtilis | ? | - |
? | |
2.6.1.1 | L-glutamate + 2-oxoglutarate | relative activity compared to L-aspartate: 46.7% | Bacillus subtilis | 2-oxoglutarate + L-glutamate | - |
? | |
2.6.1.1 | L-phenylalanine + 2-oxoglutarate | relative activity compared to L-aspartate: 0.3% | Bacillus subtilis | 2-oxo-3-phenylpropionic acid + L-glutamate | - |
? | |
2.6.1.1 | L-tryptophan + 2-oxoglutarate | relative activity compared to L-aspartate: 1.7% | Bacillus subtilis | 3-indole-2-oxopropionic acid + L-glutamate | - |
? | |
2.6.1.1 | L-tyrosine + 2-oxoglutarate | relative activity compared to L-aspartate: 0.4 | Bacillus subtilis | 3-(4-hydroxyphenyl)-2-oxopropionic acid + L-glutamate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.6.1.1 | monomer | - |
Bacillus subtilis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.6.1.1 | aatB3 | - |
Bacillus subtilis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.6.1.1 | 45 | - |
- |
Bacillus subtilis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.6.1.1 | 50 | - |
enzyme stable up to 50°C | Bacillus subtilis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.1 | 14 | - |
L-glutamate | pH 8.0, 45°C | Bacillus subtilis | |
2.6.1.1 | 22 | - |
oxaloacetate | pH 8.0, 45°C | Bacillus subtilis | |
2.6.1.1 | 27 | - |
2-oxoglutarate | pH 8.0, 45°C | Bacillus subtilis | |
2.6.1.1 | 30 | - |
L-aspartate | pH 8.0, 45°C | Bacillus subtilis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.6.1.1 | 8 | - |
- |
Bacillus subtilis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.6.1.1 | 7 | 9 | relatively high activity in alkaline range | Bacillus subtilis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.1 | pyridoxal 5'-phosphate | - |
Bacillus subtilis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.1 | 1.75 | - |
L-glutamate | pH 8.0, 45°C | Bacillus subtilis | |
2.6.1.1 | 4.5 | - |
L-aspartate | pH 8.0, 45°C | Bacillus subtilis | |
2.6.1.1 | 36.67 | - |
oxaloacetate | pH 8.0, 45°C | Bacillus subtilis | |
2.6.1.1 | 84.38 | - |
2-oxoglutarate | pH 8.0, 45°C | Bacillus subtilis |