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Literature summary extracted from

  • Nonaka, M.; Tsuchimoto, D.; Sakumi, K.; Nakabeppu, Y.
    Mouse RS21-C6 is a mammalian 2'-deoxycytidine 5'-triphosphate pyrophosphohydrolase that prefers 5-iodocytosine (2009), FEBS J., 276, 1654-1666.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.12
-
 Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.12 additional information not inhibitory: ITP up to 0.5 mM Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.12 0.0039
-
 5-iodo-dCTP pH 8.0, 30°C Mus musculus
3.6.1.12 0.0217
-
 5-bromo-dCTP pH 8.0, 30°C Mus musculus
3.6.1.12 0.034
-
 5-fluoro-dUTP pH 8.0, 30°C Mus musculus
3.6.1.12 0.0441
-
 dCTP pH 8.0, 30°C Mus musculus
3.6.1.12 0.0485
-
 5-Methyl-dCTP pH 8.0, 30°C Mus musculus
3.6.1.12 0.05
-
 5-chloro-dCTP pH 8.0, 30°C Mus musculus
3.6.1.12 0.107
-
 2-chloro-dATP pH 8.0, 30°C Mus musculus
3.6.1.12 0.118
-
 dATP pH 8.0, 30°C Mus musculus
3.6.1.12 0.164
-
 5-hydroxy-dCTP pH 8.0, 30°C Mus musculus
3.6.1.12 0.169
-
 2-hydroxy-dATP pH 8.0, 30°C Mus musculus
3.6.1.12 0.407
-
 dTTP pH 8.0, 30°C Mus musculus
3.6.1.12 0.484
-
 dUTP pH 8.0, 30°C Mus musculus
3.6.1.12 0.529
-
 CTP pH 8.0, 30°C Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.12 cytosol exclusivelyx located in the cytosol Mus musculus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.12 MgCl2 maximum activity in presence of 100 mM MgCl2 Mus musculus
3.6.1.12 MnCl2 up to 40% of the activity with MgCl2 Mus musculus
3.6.1.12 additional information divalent cation is absolutely required Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.12 18783
-
 x * 19000, SDS-PAGE, x * 18783, calculated Mus musculus
3.6.1.12 19000
-
 x * 19000, SDS-PAGE, x * 18783, calculated Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.12 Mus musculus Q9QY93
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.12 heart enzyme is ubiquitously expressed. Expression is highest in the liver and heart, and to a lesser extent the salivary gland Mus musculus
-
3.6.1.12 liver enzyme is ubiquitously expressed. Expression is highest in the liver and heart, and to a lesser extent the salivary gland Mus musculus
-
3.6.1.12 salivary gland enzyme is ubiquitously expressed. Expression is highest in the liver and heart, and to a lesser extent the salivary gland Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.12 2-chloro-dATP + H2O
-
 Mus musculus 2-chloro-AMP + diphosphate
-
 ?
3.6.1.12 2-hydroxy-dATP + H2O
-
 Mus musculus 2-hydroxy-dAMP + diphosphate
-
 ?
3.6.1.12 5-bromo-dCTP + H2O
-
 Mus musculus 5-bromo-dCMP + diphosphate
-
 ?
3.6.1.12 5-chloro-dCTP + H2O
-
 Mus musculus 5-chloro-dCMP + diphosphate
-
 ?
3.6.1.12 5-fluoro-dUTP + H2O
-
 Mus musculus 5-fluoro-dUMP + diphosphate
-
 ?
3.6.1.12 5-hydroxy-dCTP + H2O
-
 Mus musculus 5-hydroxy-dCMP + diphosphate
-
 ?
3.6.1.12 5-iodo-dCTP + H2O
-
 Mus musculus 5-iodo-dCMP + diphosphate
-
 ?
3.6.1.12 5-methyl-dCTP + H2O
-
 Mus musculus 5-methyl-dCMP + diphosphate
-
 ?
3.6.1.12 CTP + H2O
-
 Mus musculus CMP + diphosphate
-
 ?
3.6.1.12 dATP + H2O
-
 Mus musculus dAMP + diphosphate
-
 ?
3.6.1.12 dCTP + H2O
-
 Mus musculus dCMP + diphosphate
-
 ?
3.6.1.12 dTTP + H2O
-
 Mus musculus dTMP + diphosphate
-
 ?
3.6.1.12 dUTP + H2O
-
 Mus musculus dUMP + diphosphate
-
 ?
3.6.1.12 additional information no substrates: ATP, GTP, UTP, ITP, 2-hydroxy-ATP, dGTP, dITP, 8-oxo-dGTP, dCDP, dADP Mus musculus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.12 ? x * 19000, SDS-PAGE, x * 18783, calculated Mus musculus

Synonyms

EC Number Synonyms Comment Organism
3.6.1.12 dCTP pyrophosphatase 1
-
 Mus musculus
3.6.1.12 dCTPase 1
-
 Mus musculus
3.6.1.12 DCTPP1
-
 Mus musculus
3.6.1.12 RS21-C6
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.12 60
-
 temperature-dependent increase of activity up to 60°C Mus musculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.12 0.31
-
 CTP pH 8.0, 30°C Mus musculus
3.6.1.12 0.53
-
 dATP pH 8.0, 30°C Mus musculus
3.6.1.12 0.58
-
 5-fluoro-dUTP pH 8.0, 30°C Mus musculus
3.6.1.12 0.76
-
 dTTP pH 8.0, 30°C Mus musculus
3.6.1.12 0.76
-
 dUTP pH 8.0, 30°C Mus musculus
3.6.1.12 0.87
-
 5-hydroxy-dCTP pH 8.0, 30°C Mus musculus
3.6.1.12 0.94
-
 5-iodo-dCTP pH 8.0, 30°C Mus musculus
3.6.1.12 1.33
-
 5-Methyl-dCTP pH 8.0, 30°C Mus musculus
3.6.1.12 1.37
-
 dCTP pH 8.0, 30°C Mus musculus
3.6.1.12 1.39
-
 2-hydroxy-dATP pH 8.0, 30°C Mus musculus
3.6.1.12 1.49
-
 5-bromo-dCTP pH 8.0, 30°C Mus musculus
3.6.1.12 1.5
-
 5-chloro-dCTP pH 8.0, 30°C Mus musculus
3.6.1.12 1.57
-
 2-chloro-dATP pH 8.0, 30°C Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.12 9.5
-
-
 Mus musculus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.6.1.12 0.156
-
 CTP pH 8.0, 30°C Mus musculus
3.6.1.12 1.57
-
 dUTP pH 8.0, 30°C Mus musculus
3.6.1.12 1.87
-
 dTTP pH 8.0, 30°C Mus musculus
3.6.1.12 1.9
-
 5-fluoro-dUTP pH 8.0, 30°C Mus musculus
3.6.1.12 4.49
-
 dATP pH 8.0, 30°C Mus musculus
3.6.1.12 5.3
-
 5-hydroxy-dCTP pH 8.0, 30°C Mus musculus
3.6.1.12 8.22
-
 2-hydroxy-dATP pH 8.0, 30°C Mus musculus
3.6.1.12 14.7
-
 2-chloro-dATP pH 8.0, 30°C Mus musculus
3.6.1.12 27.4
-
 5-Methyl-dCTP pH 8.0, 30°C Mus musculus
3.6.1.12 30
-
 5-chloro-dCTP pH 8.0, 30°C Mus musculus
3.6.1.12 31.1
-
 dCTP pH 8.0, 30°C Mus musculus
3.6.1.12 68.7
-
 5-bromo-dCTP pH 8.0, 30°C Mus musculus
3.6.1.12 241
-
 5-iodo-dCTP pH 8.0, 30°C Mus musculus