Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sillero, M.A.G.; Montes, M.; de Diego, A.; del Valle, M.; Atencia, E.A.; Sillero, A.
    Thermostable Pyrococcus furiosus DNA ligase catalyzes the synthesis of (di)nucleoside polyphosphates (2002), Extremophiles, 6, 45-50.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.5.1.1 molecular biology the enzyme is used in the ligase chain reaction Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.5.1.1 0.001
-
 ATP pH 7.5, 70°C, synthesis of P1-(5'-adenosyl),P4-(5'-guanosyl)tetraphosphate Pyrococcus furiosus
6.5.1.1 0.4
-
 GTP pH 7.5, 70°C, synthesis of P1-(5'-adenosyl),P4-(5'-guanosyl)tetraphosphate Pyrococcus furiosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.1 Mg2+ optimal concentration: 4 mM Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.1 Pyrococcus furiosus P56709
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.1 ATP + ADP
-
 Pyrococcus furiosus P1-(5'-adenosyl),P3-(5'-adenosyl)triphosphate + diphosphate
-
 ?
6.5.1.1 ATP + CDP
-
 Pyrococcus furiosus P1-(5'-adenosyl),P3-(5'-cytidyl)triphosphate + diphosphate
-
 ?
6.5.1.1 ATP + CTP
-
 Pyrococcus furiosus P1-(5'-adenosyl),P4-(5'-cytidyl)tetraphosphate + diphosphate
-
 ?
6.5.1.1 ATP + dCTP
-
 Pyrococcus furiosus P1-(5'-adenosyl),P4-[5'-(2'-deoxycytidyl)]tetraphosphate + diphosphate
-
 ?
6.5.1.1 ATP + dGTP
-
 Pyrococcus furiosus P1-(5'-adenosyl),P4-[5'-(2'-deoxyguanosyl)]tetraphosphate + diphosphate
-
 ?
6.5.1.1 ATP + dTTP
-
 Pyrococcus furiosus P1-(5'-adenosyl),P4-(5'-thymidyl)tetraphosphate + diphosphate
-
 ?
6.5.1.1 ATP + GDP
-
 Pyrococcus furiosus P1-(5'-adenosyl),P3-(5'-guanosyl)triphosphate + diphosphate
-
 ?
6.5.1.1 ATP + GTP
-
 Pyrococcus furiosus P1-(5'-adenosyl),P4-(5'-guanosyl)tetraphosphate + diphosphate
-
 ?
6.5.1.1 ATP + UTP
-
 Pyrococcus furiosus P1-(5'-adenosyl),P4-(5'-uridinyl)tetraphosphate + diphosphate
-
 ?
6.5.1.1 ATP + XDP
-
 Pyrococcus furiosus P1-(5'-adenosyl),P4-(5'-xanthosyl)tetraphosphate + diphosphate
-
 ?
6.5.1.1 ATP + XTP
-
 Pyrococcus furiosus P1-(5'-adenosyl),P4-(5'-xanthosyl)tetraphosphate + diphosphate
-
 ?
6.5.1.1 additional information the rate of synthesis of Ap4N is double that of the corresponding Ap3N Pyrococcus furiosus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.5.1.1 DNA ligase
-
 Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.5.1.1 70
-
-
 Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5.1.1 7.2 7.5
-
 Pyrococcus furiosus