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Literature summary extracted from

  • Liu, T.; Hao, L.; Wang, R.; Liu, B.
    Molecular characterization of a thermostable aldehyde dehydrogenase (ALDH) from the hyperthermophilic archaeon Sulfolobus tokodaii strain 7 (2013), Extremophiles, 17, 181-190.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.3 2-mercaptoethanol about 140% activity at 5 mM Sulfurisphaera tokodaii
1.2.1.3 dithiothreitol about 150% activity at 5 mM Sulfurisphaera tokodaii
1.2.1.5 NH4+ 500 mM, 1.31fold activation Sulfurisphaera tokodaii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.3 expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells Sulfurisphaera tokodaii
1.2.1.5
-
 Sulfurisphaera tokodaii

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.3 C274A the mutation leads to a drastic loss of the activity Sulfurisphaera tokodaii
1.2.1.3 C274S the mutation leads to a drastic loss of the activity Sulfurisphaera tokodaii
1.2.1.3 E240A the mutation leads to a drastic loss of the activity Sulfurisphaera tokodaii
1.2.1.3 E240S the mutation leads to a drastic loss of the activity Sulfurisphaera tokodaii
1.2.1.3 E370Q the mutation results in decreased activity (higher Km and lower kcat values toward NAD+ and acetaldehyde than the wild type enzyme) Sulfurisphaera tokodaii
1.2.1.3 I168A the mutation results in no obvious change of kinetics properties toward acetaldehyde and a comparable increase of affinity toward NAD+ Sulfurisphaera tokodaii
1.2.1.3 K165Q the mutation results in decreased activity (higher Km and lower kcat values toward NAD+ and acetaldehyde than the wild type enzyme) Sulfurisphaera tokodaii
1.2.1.3 N142A the mutation results in decreased activity (higher Km and lower kcat values toward NAD+ and acetaldehyde than the wild type enzyme) Sulfurisphaera tokodaii

General Stability

EC Number General Stability Organism
1.2.1.5 the enzyme is stable to 50 mM PMSF Sulfurisphaera tokodaii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.3 H2O2
-
 Sulfurisphaera tokodaii
1.2.1.3 nitroglycerin more than half of the original activity retained in the presence of 5.5 mM and approximate 80% of the activity is lost by the addition of 11 mM nitroglycerin Sulfurisphaera tokodaii
1.2.1.5 2-mercaptoethanol 10% (v/v), 55% inhibition Sulfurisphaera tokodaii
1.2.1.5 acetone 10% (v/v), 21% inhibition Sulfurisphaera tokodaii
1.2.1.5 DMSO 10% (v/v), 32% inhibition Sulfurisphaera tokodaii
1.2.1.5 EDTA 50 mM, 22% inhibition Sulfurisphaera tokodaii
1.2.1.5 ethanediol 10% (v/v), 60% inhibition Sulfurisphaera tokodaii
1.2.1.5 guanidine hydrochloride 10% (v/v), 42% inhibition Sulfurisphaera tokodaii
1.2.1.5 H2O2 the loss of activity induced by H2O2 can be restored by reducing agents, although a decrease of the restored activity is observed upon addition of 10 mM dithiothreitol Sulfurisphaera tokodaii
1.2.1.5 nitroglycerin more than half of the original activity is retained in the presence of 5.5 mM nitroglycerin and approximate 80% of the activity is lost by the addition of 11 mM nitroglycerin. Dithiothreitol and alpha-lipoic acid can also restore the nitroglycerin inactivated dehydrogenation to more than half of the original activity Sulfurisphaera tokodaii
1.2.1.5 PEG-400 10% (v/v), 16% inhibition Sulfurisphaera tokodaii
1.2.1.5 PMSF 50 mM, no loss of activity Sulfurisphaera tokodaii
1.2.1.5 Urea 500 mM, 44% inhibition Sulfurisphaera tokodaii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.3 0.82
-
 NAD+ with Dl-glyceraldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C Sulfurisphaera tokodaii
1.2.1.3 0.84
-
 NAD+ with acetaldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C Sulfurisphaera tokodaii
1.2.1.3 3.81
-
 NADP+ with acetaldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C Sulfurisphaera tokodaii
1.2.1.3 6.12
-
 DL-glyceraldehyde 3-phosphate in 50 mM Tris/HCl, at pH 8.0 and 80°C Sulfurisphaera tokodaii
1.2.1.3 6.38
-
 acetaldehyde in 50 mM Tris/HCl, at pH 8.0 and 80°C Sulfurisphaera tokodaii
1.2.1.3 8.47
-
 NADP+ with Dl-glyceraldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C Sulfurisphaera tokodaii
1.2.1.3 8.51
-
 DL-glyceraldehyde in 50 mM Tris/HCl, at pH 8.0 and 80°C Sulfurisphaera tokodaii
1.2.1.5 0.84
-
 NAD+ pH 8, 80°C Sulfurisphaera tokodaii
1.2.1.5 3.81
-
 NADP+ pH 8, 80°C Sulfurisphaera tokodaii
1.2.1.5 6.12
-
 D-glyceraldehyde 3-phosphate pH 8, 80°C Sulfurisphaera tokodaii
1.2.1.5 6.38
-
 acetaldehyde pH 8, 80°C Sulfurisphaera tokodaii
1.2.1.5 8.51
-
 D-glyceraldehyde pH 8, 80°C Sulfurisphaera tokodaii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.3 K+ 130% activity at 20 mM Sulfurisphaera tokodaii
1.2.1.3 Li+ 119% activity at 20 mM Sulfurisphaera tokodaii
1.2.1.3 additional information the enzyme is not influenced by 20 mM Mg2, Cu2+, Ca2+, Ba2+, Ni2+, Co2+, and Sr2+ Sulfurisphaera tokodaii
1.2.1.3 Na+ 122% activity at 20 mM Sulfurisphaera tokodaii
1.2.1.3 NH4+ 131% activity at 20 mM Sulfurisphaera tokodaii
1.2.1.5 K+ 500 mM, 1.3fold activation Sulfurisphaera tokodaii
1.2.1.5 Li+ 500 mM, 107% of the activity compared to activity without metal ions Sulfurisphaera tokodaii
1.2.1.5 Mg2+ 20 mM, 107% of the activity compared to activity without metal ions Sulfurisphaera tokodaii
1.2.1.5 Na+ 500 mM, 1.2fold activation Sulfurisphaera tokodaii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.3 50000
-
 4 * 50000, SDS-PAGE Sulfurisphaera tokodaii
1.2.1.3 51300
-
 4 * 51300, calculated from amino acid sequence Sulfurisphaera tokodaii
1.2.1.3 200000
-
 gel filtration Sulfurisphaera tokodaii
1.2.1.5 50000
-
 4 * 50000, SDS-PAGE Sulfurisphaera tokodaii
1.2.1.5 51300
-
 4 * 51300, calculation from sequence Sulfurisphaera tokodaii
1.2.1.5 200000
-
 gel filtration Sulfurisphaera tokodaii

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.2.1.3 dithiothreitol the enzyme is stable in dithiothreitol (90% of the original activity remaining) Sulfurisphaera tokodaii
1.2.1.3 Ethanol the enzyme is stable in ethanol (90% of the original activity remaining) Sulfurisphaera tokodaii
1.2.1.3 isopropanol the enzyme is stable in isopropanol (90% of the original activity remaining) Sulfurisphaera tokodaii
1.2.1.3 Methanol the enzyme is stable in methanol (90% of the original activity remaining) Sulfurisphaera tokodaii
1.2.1.3 phenylmethylsulfonyl fluoride the enzyme is stable in phenylmethylsulfonyl fluoride (90% of the original activity remaining) Sulfurisphaera tokodaii
1.2.1.3 SDS the enzyme is stable in SDS (90% of the original activity remaining) Sulfurisphaera tokodaii
1.2.1.5 2-mercaptoethanol 10% (v/v), 55% loss of activity Sulfurisphaera tokodaii
1.2.1.5 Acetone 10% (v/v), 21% loss of activity Sulfurisphaera tokodaii
1.2.1.5 DMSO 10% (v/v),% loss of activity Sulfurisphaera tokodaii
1.2.1.5 Ethanol 10% (v/v), 2% loss of activity Sulfurisphaera tokodaii
1.2.1.5 Ethylene glycol 10% (v/v), 60% loss of activity Sulfurisphaera tokodaii
1.2.1.5 guanidine-HCl 10% (v/v), 42% loss of activity Sulfurisphaera tokodaii
1.2.1.5 isopropanol 10% (v/v), 8% loss of activity Sulfurisphaera tokodaii
1.2.1.5 Methanol 10% (v/v), 7% loss of activity Sulfurisphaera tokodaii
1.2.1.5 polyethyleneglycol PEG-400, 10% (v/v), 16% loss of activity Sulfurisphaera tokodaii
1.2.1.5 SDS 10% (v/v), 3% loss of activity Sulfurisphaera tokodaii
1.2.1.5 urea 500 mM, 44% loss of activity Sulfurisphaera tokodaii

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.3 Sulfurisphaera tokodaii
-
-
-
1.2.1.3 Sulfurisphaera tokodaii 7
-
-
-
1.2.1.5 Sulfurisphaera tokodaii Q976X5
-
-
1.2.1.5 Sulfurisphaera tokodaii 7 Q976X5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.3 Ni-NTA affinity column chromatography Sulfurisphaera tokodaii
1.2.1.5
-
 Sulfurisphaera tokodaii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.3 1,3-dihydroxyacetone + NAD+ + H2O 53.93% activity compared to acetaldehyde Sulfurisphaera tokodaii ? + NADH + H+
-
 ?
1.2.1.3 3-nitrobenzaldehyde + NAD+ + H2O 32.98% activity compared to acetaldehyde Sulfurisphaera tokodaii 3-nitrobenzoate + NADH + H+
-
 ?
1.2.1.3 acetaldehyde + NAD+ + H2O 100% activity with acetaldehyde and NAD+ Sulfurisphaera tokodaii acetate + NADH + H+
-
 ?
1.2.1.3 acetaldehyde + NAD+ + H2O 100% activity with acetaldehyde and NAD+ Sulfurisphaera tokodaii 7 acetate + NADH + H+
-
 ?
1.2.1.3 acetaldehyde + NADP+ + H2O low activity with NADP+ Sulfurisphaera tokodaii acetate + NADPH + H+
-
 ?
1.2.1.3 benzaldehyde + NAD+ + H2O 35.5% activity compared to acetaldehyde Sulfurisphaera tokodaii benzoate + NADH + H+
-
 ?
1.2.1.3 benzaldehyde + NAD+ + H2O 35.5% activity compared to acetaldehyde Sulfurisphaera tokodaii 7 benzoate + NADH + H+
-
 ?
1.2.1.3 butyraldehyde + NAD+ + H2O 48.15% activity compared to acetaldehyde Sulfurisphaera tokodaii butyrate + NADH + H+
-
 ?
1.2.1.3 butyraldehyde + NAD+ + H2O 48.15% activity compared to acetaldehyde Sulfurisphaera tokodaii 7 butyrate + NADH + H+
-
 ?
1.2.1.3 chloral + NAD+ + H2O 2.67% activity compared to acetaldehyde Sulfurisphaera tokodaii trichloroacetate + NADH + H+
-
 ?
1.2.1.3 D-glyceraldehyde + NAD+ + H2O 50.25% activity compared to acetaldehyde Sulfurisphaera tokodaii D-glycerate + NADH + H+
-
 ?
1.2.1.3 DL-glyceraldehyde + NAD+ + H2O 77.27% activity compared to acetaldehyde Sulfurisphaera tokodaii DL-glycerate + NADH + H+
-
 ?
1.2.1.3 DL-glyceraldehyde + NAD+ + H2O low activity with NADP+ Sulfurisphaera tokodaii DL-glycerate + NADH + H+
-
 ?
1.2.1.3 DL-glyceraldehyde 3-phosphate + NAD+ + H2O 52.7% activity compared to acetaldehyde Sulfurisphaera tokodaii DL-glycerate 3-phosphate + NADH + H+
-
 ?
1.2.1.3 formaldehyde + NAD+ + H2O 7.77% activity compared to acetaldehyde Sulfurisphaera tokodaii formate + NADH + H+
-
 ?
1.2.1.3 glycolaldehyde + NAD+ + H2O 75.43% activity compared to acetaldehyde Sulfurisphaera tokodaii glycolate + NADH + H+
-
 ?
1.2.1.3 additional information no activity with methanol, ethanol and isopropanol Sulfurisphaera tokodaii ?
-
 ?
1.2.1.3 additional information no activity with methanol, ethanol and isopropanol Sulfurisphaera tokodaii 7 ?
-
 ?
1.2.1.3 phenylacetaldehyde + NAD+ + H2O 4.21% activity compared to acetaldehyde Sulfurisphaera tokodaii phenylacetate + NADH + H+
-
 ?
1.2.1.3 propionaldehyde + NAD+ + H2O 56.22% activity compared to acetaldehyde Sulfurisphaera tokodaii propionate + NADH + H+
-
 ?
1.2.1.3 propionaldehyde + NAD+ + H2O 56.22% activity compared to acetaldehyde Sulfurisphaera tokodaii 7 propionate + NADH + H+
-
 ?
1.2.1.5 1,3-dihydroxyacetone + NAD+ + H2O 53.93% of the activity compared to acetaldehyde Sulfurisphaera tokodaii ? + NADH + H+
-
 ?
1.2.1.5 3-nitrobenzaldehyde + NAD+ + H2O 32.98% of the activity compared to acetaldehyde Sulfurisphaera tokodaii 3-nitrobenzoate + NADH + H+
-
 ?
1.2.1.5 acetaldehyde + NAD+ + H2O kcat/KM for NAD+ with acetaldehyde as cosubstrate is 2.5fold higher compared to NADP+ Sulfurisphaera tokodaii acetate + NADH + H+
-
 ?
1.2.1.5 acetaldehyde + NAD+ + H2O kcat/KM for NAD+ with acetaldehyde as cosubstrate is 2.5fold higher compared to NADP+ Sulfurisphaera tokodaii 7 acetate + NADH + H+
-
 ?
1.2.1.5 acetaldehyde + NADP+ + H2O
-
 Sulfurisphaera tokodaii acetate + NADPH
-
 ?
1.2.1.5 benzaldehyde + NAD+ + H2O 35.5% of the activity compared to acetaldehyde Sulfurisphaera tokodaii benzoate + NADH + H+
-
 ?
1.2.1.5 benzaldehyde + NAD+ + H2O 35.5% of the activity compared to acetaldehyde Sulfurisphaera tokodaii 7 benzoate + NADH + H+
-
 ?
1.2.1.5 butyraldehyde + NAD+ + H2O 48.15% of the activity compared to acetaldehyde Sulfurisphaera tokodaii butyrate + NADH + H+
-
 ?
1.2.1.5 butyraldehyde + NAD+ + H2O 48.15% of the activity compared to acetaldehyde Sulfurisphaera tokodaii 7 butyrate + NADH + H+
-
 ?
1.2.1.5 D-glyceraldehyde + NAD+ + H2O 50.25% of the activity compared to acetaldehyde. kcat/KM for NAD+ with D-glyceraldehyde as cosubstrate is 3.8fold higher compared to NADP+ Sulfurisphaera tokodaii D-glycerate + NADH + H+
-
 ?
1.2.1.5 D-glyceraldehyde + NADP+ + H2O 50.25% of the activity compared to acetaldehyde Sulfurisphaera tokodaii D-glycerate + NADPH
-
 ?
1.2.1.5 D-glyceraldehyde 3-phosphate + NAD+ + H2O 52.7% of the activity compared to acetaldehyde Sulfurisphaera tokodaii D-glycerate 3-phosphate + NADH + H+
-
 ?
1.2.1.5 glycolaldehyde + NAD+ + H2O 75.43% of the activity compared to acetaldehyde Sulfurisphaera tokodaii glycolate + NADH + H+
-
 ?
1.2.1.5 additional information no activity with methanol or ethanol Sulfurisphaera tokodaii ?
-
 ?
1.2.1.5 additional information no activity with methanol or ethanol Sulfurisphaera tokodaii 7 ?
-
 ?
1.2.1.5 propionaldehyde + NAD+ + H2O 56.22% of the activity compared to acetaldehyde Sulfurisphaera tokodaii propionate + NADH + H+
-
 ?
1.2.1.5 propionaldehyde + NAD+ + H2O 56.22% of the activity compared to acetaldehyde Sulfurisphaera tokodaii 7 propionate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.3 homotetramer 4 * 50000, SDS-PAGE Sulfurisphaera tokodaii
1.2.1.3 homotetramer 4 * 51300, calculated from amino acid sequence Sulfurisphaera tokodaii
1.2.1.5 tetramer 4 * 50000, SDS-PAGE Sulfurisphaera tokodaii
1.2.1.5 tetramer 4 * 51300, calculation from sequence Sulfurisphaera tokodaii

Synonyms

EC Number Synonyms Comment Organism
1.2.1.3 ALDH
-
 Sulfurisphaera tokodaii
1.2.1.5 ST0064
-
 Sulfurisphaera tokodaii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.3 80
-
-
 Sulfurisphaera tokodaii
1.2.1.5 80
-
-
 Sulfurisphaera tokodaii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.2.1.3 70 90 about 50% activity at 70°C, 100% activity at 80°C, about 80% activity at 90°C Sulfurisphaera tokodaii
1.2.1.5 70 90 70°C: about 50% of maximal activity, 90°C: about 90% of maximal activity Sulfurisphaera tokodaii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.3 70 90 the enzyme shows no obvious loss of activity after heat-treatment at 70°C for 12 h, and more than 60% of the original activity remains after treatment at 80°C for 12 h. The half-life at 90°C is 4 h Sulfurisphaera tokodaii
1.2.1.5 additional information
-
 coenzyme NAD+ or NADP+ can regulate the enzymatic thermostability Sulfurisphaera tokodaii
1.2.1.5 70 80 about 20% increase of activity after treatment at 70 and 80°C for 2 h, probably due to thermal activation of the enzyme Sulfurisphaera tokodaii
1.2.1.5 90
-
 half-life: 4 h. Significant protection against thermal inactivation by 1 mM NAD+ or NADP+ when the preheating time is above 4 h at 90°C Sulfurisphaera tokodaii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.3 0.6
-
 NAD+ with DL-glyceraldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C Sulfurisphaera tokodaii
1.2.1.3 0.83
-
 DL-glyceraldehyde 3-phosphate in 50 mM Tris/HCl, at pH 8.0 and 80°C Sulfurisphaera tokodaii
1.2.1.3 0.92
-
 DL-glyceraldehyde in 50 mM Tris/HCl, at pH 8.0 and 80°C Sulfurisphaera tokodaii
1.2.1.3 1.14
-
 acetaldehyde in 50 mM Tris/HCl, at pH 8.0 and 80°C Sulfurisphaera tokodaii
1.2.1.3 1.63
-
 NADP+ with DL-glyceraldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C Sulfurisphaera tokodaii
1.2.1.3 2.05
-
 NAD+ with acetaldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C Sulfurisphaera tokodaii
1.2.1.3 3.71
-
 NADP+ with acetaldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C Sulfurisphaera tokodaii
1.2.1.5 0.83
-
 D-glyceraldehyde 3-phosphate pH 8, 80°C Sulfurisphaera tokodaii
1.2.1.5 0.92
-
 D-glyceraldehyde pH 8, 80°C Sulfurisphaera tokodaii
1.2.1.5 1.14
-
 acetaldehyde pH 8, 80°C Sulfurisphaera tokodaii
1.2.1.5 2.05
-
 NAD+ pH 8, 80°C Sulfurisphaera tokodaii
1.2.1.5 3.71
-
 NADP+ pH 8, 80°C Sulfurisphaera tokodaii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.3 8
-
-
 Sulfurisphaera tokodaii
1.2.1.5 8
-
-
 Sulfurisphaera tokodaii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.3 6 9 about 60% activity at pH 6.0, about 70% activity at pH 7.0, 100% activity pH 8.0, about 85% activity at pH 9.0 Sulfurisphaera tokodaii
1.2.1.5 6 9 pH 6.0: about 60% of maximal activity, pH 9.0: about 90% of maximal activity Sulfurisphaera tokodaii

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.3 NAD+ the enzyme can utilize both NAD+ and NADP+ as cofactors and shows a preference toward NAD+ Sulfurisphaera tokodaii
1.2.1.3 NADP+ the enzyme can utilize both NAD+ and NADP+ as cofactors and shows a preference toward NAD+ Sulfurisphaera tokodaii
1.2.1.5 NAD+ kcat/KM for NAD+ with acetaldehyde as cosubstrate is 2.5fold higher compared to NADP+. kcat/KM for NAD+ with D-glyceraldehyde as cosubstrate is 3.8fold higher compared to NADP+ Sulfurisphaera tokodaii
1.2.1.5 NADP+ kcat/KM for NAD+ with acetaldehyde as cosubstrate is 2.5fold higher compared to NADP+. kcat/KM for NAD+ with D-glyceraldehyde as cosubstrate is 3.8fold higher compared to NADP+ Sulfurisphaera tokodaii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2.1.3 0.11
-
 DL-glyceraldehyde in 50 mM Tris/HCl, at pH 8.0 and 80°C Sulfurisphaera tokodaii
1.2.1.3 0.14
-
 DL-glyceraldehyde 3-phosphate in 50 mM Tris/HCl, at pH 8.0 and 80°C Sulfurisphaera tokodaii
1.2.1.3 0.18
-
 acetaldehyde in 50 mM Tris/HCl, at pH 8.0 and 80°C Sulfurisphaera tokodaii
1.2.1.3 0.19
-
 NADP+ with DL-glyceraldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C Sulfurisphaera tokodaii
1.2.1.3 0.73
-
 NAD+ with DL-glyceraldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C Sulfurisphaera tokodaii
1.2.1.3 0.98
-
 NADP+ with acetaldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C Sulfurisphaera tokodaii
1.2.1.3 2.45
-
 NAD+ with acetaldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C Sulfurisphaera tokodaii
1.2.1.5 0.11
-
 D-glyceraldehyde pH 8, 80°C Sulfurisphaera tokodaii
1.2.1.5 0.14
-
 D-glyceraldehyde 3-phosphate pH 8, 80°C Sulfurisphaera tokodaii
1.2.1.5 0.18
-
 acetaldehyde pH 8, 80°C Sulfurisphaera tokodaii
1.2.1.5 0.98
-
 NADP+ pH 8, 80°C Sulfurisphaera tokodaii
1.2.1.5 2.45
-
 NAD+ pH 8, 80°C Sulfurisphaera tokodaii