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Literature summary extracted from

  • van der Oost, J.; Voorhorst, W.G.; Kengen, S.W.; Geerling, A.C.; Wittenhorst, V.; Gueguen, Y.; de Vos, W.M.
    Genetic and biochemical characterization of a short-chain alcohol dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus (2001), Eur. J. Biochem., 268, 3062-3068.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.2 expressed in Escherichia coli Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.2 0.08
-
NADPH pH 7.0, 70°C Pyrococcus furiosus
1.1.1.2 0.08
-
NADP+ pH 10.0, 70°C Pyrococcus furiosus
1.1.1.2 3.3
-
Pyruvaldehyde pH 7.0, 70°C Pyrococcus furiosus
1.1.1.2 60.8
-
2-Pentanol pH 10.0, 70°C Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.2 25962
-
2 * 25962, calculated from sequence Pyrococcus furiosus
1.1.1.2 26000
-
2 * 26000, SDS-PAGE Pyrococcus furiosus
1.1.1.2 50000 55000 gel filtration Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.2 Pyrococcus furiosus O73949
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.2
-
Pyrococcus furiosus

Storage Stability

EC Number Storage Stability Organism
1.1.1.2 4°C, several months, stable Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.2 (R)-2-butanol + NADP+ the enzyme shows a slight preference for the (S)-2-butanol over (R)-2-butanol Pyrococcus furiosus 2-butanone + NADPH + H+
-
?
1.1.1.2 (S)-2-butanol + NADP+ the enzyme shows a slight preference for the (S)-2-butanol over (R)-2-butanol Pyrococcus furiosus 2-butanone + NADPH + H+
-
?
1.1.1.2 2-pentanol + NADP+ maximal activity in oxidative reaction Pyrococcus furiosus 2-pentanone + NADPH + H+
-
r
1.1.1.2 2-propanol + NADP+
-
Pyrococcus furiosus acetone + NADPH + H+
-
?
1.1.1.2 additional information a broad range of primary alcohols could be oxidized by AdhA and includes substrates with 2-10 carbon atoms. It shows activity towards a broad range of secondary alcohols that on average are oxidized faster than the primary alcohols. Alcohols with a second or a third hydroxyl group are converted less efficiently Pyrococcus furiosus ?
-
?
1.1.1.2 pyruvaldehyde + NADPH + H+ maximal activity in reductive reaction Pyrococcus furiosus lactaldehyde + NADP+
-
r

Subunits

EC Number Subunits Comment Organism
1.1.1.2 dimer 2 * 26000, SDS-PAGE Pyrococcus furiosus
1.1.1.2 dimer 2 * 25962, calculated from sequence Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.2 AdhA
-
Pyrococcus furiosus
1.1.1.2 short-chain ADH
-
Pyrococcus furiosus
1.1.1.2 short-chain alcohol dehydrogenase
-
Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.2 70
-
assay at Pyrococcus furiosus
1.1.1.2 90
-
-
Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.2 80
-
half-life: 150 h Pyrococcus furiosus
1.1.1.2 90
-
half-life: 22.5 h Pyrococcus furiosus
1.1.1.2 100
-
half-life: 25 min Pyrococcus furiosus
1.1.1.2 103
-
melting temperature is 102.7°C Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.2 7.5
-
aldehyde reduction Pyrococcus furiosus
1.1.1.2 10 11 alcohol oxidation Pyrococcus furiosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.2 6.7 9 pH 6.7: about 80% of maximal activity, pH 9.0: about 80% of maximal activity Pyrococcus furiosus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.2 NADP+
-
Pyrococcus furiosus
1.1.1.2 NADPH
-
Pyrococcus furiosus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.2 0.3
-
2-Pentanol pH 10.0, 70°C Pyrococcus furiosus
1.1.1.2 4.2
-
Pyruvaldehyde pH 7.0, 70°C Pyrococcus furiosus