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Literature summary extracted from

  • Brown, D.M.; Upcroft, J.A.; Upcroft, P.
    A H2O-producing NADH oxidase from the protozoan parasite Giardia duodenalis (1996), Eur. J. Biochem., 241, 155-161.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.3.2 CuSO4 0.2 mM, 97% inhibition Giardia intestinalis
1.6.3.2 H2O2 0.5 mM, 50% inhibition Giardia intestinalis
1.6.3.2 HgCl2 0.5 mM, 80% inhibition Giardia intestinalis
1.6.3.2 additional information no inhibition by EDTA Giardia intestinalis
1.6.3.2 p-chloromercuribenzoate 0.05 mM, complete inhibition Giardia intestinalis
1.6.3.2 Quinacrine 1 mM, 73% inhibition Giardia intestinalis
1.6.3.2 Quinine 0.2 mM, 50% inhibition Giardia intestinalis
1.6.3.2 ZnSO4 0.1 mM, 98% inhibition Giardia intestinalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.3.2 0.0042
-
 NADH pH 7.8, 22°C Giardia intestinalis
1.6.3.2 0.016
-
 NADPH pH 7.8, 22°C Giardia intestinalis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.6.3.2 additional information activity is not enhanced by the addition of various heavy metals which suggests that the enzyme does not contain any metal ions as co-factors Giardia intestinalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.3.2 45116
-
 1 * 45116, calculated from sequence Giardia intestinalis
1.6.3.2 46000
-
 1 * 46000, SDS-PAGE Giardia intestinalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.3.2 2 NADPH + 2 H+ + O2 Giardia intestinalis the enzyme may be involved in maintenance of an optimum intracellular redox ratio 2 NADP+ + 2 H2O
-
 ?
1.6.3.2 2 NADPH + 2 H+ + O2 Giardia intestinalis BRIS/83/HEPU/106 the enzyme may be involved in maintenance of an optimum intracellular redox ratio 2 NADP+ + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.3.2 Giardia intestinalis Q7M4I9
-
-
1.6.3.2 Giardia intestinalis BRIS/83/HEPU/106 Q7M4I9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.3.2
-
 Giardia intestinalis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.3.2 trophozoite
-
 Giardia intestinalis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.3.2 2 NADH + H+ + O2 NADPH is more efficient as electron donor compared to NADH Giardia intestinalis NAD+ + 2 H2O
-
 ?
1.6.3.2 2 NADH + H+ + O2 NADPH is more efficient as electron donor compared to NADH Giardia intestinalis BRIS/83/HEPU/106 NAD+ + 2 H2O
-
 ?
1.6.3.2 2 NADPH + 2 H+ + O2 the enzyme may be involved in maintenance of an optimum intracellular redox ratio Giardia intestinalis 2 NADP+ + 2 H2O
-
 ?
1.6.3.2 2 NADPH + 2 H+ + O2 O2 is the most effective electron acceptor, NADH or NADPH are not oxidized anaerobically Giardia intestinalis 2 NADP+ + 2 H2O
-
 ?
1.6.3.2 2 NADPH + 2 H+ + O2 the enzyme may be involved in maintenance of an optimum intracellular redox ratio Giardia intestinalis BRIS/83/HEPU/106 2 NADP+ + 2 H2O
-
 ?
1.6.3.2 2 NADPH + 2 H+ + O2 O2 is the most effective electron acceptor, NADH or NADPH are not oxidized anaerobically Giardia intestinalis BRIS/83/HEPU/106 2 NADP+ + 2 H2O
-
 ?
1.6.3.2 additional information cytochrome c is not reduced under any assay conditions used Giardia intestinalis ?
-
 ?
1.6.3.2 additional information cytochrome c is not reduced under any assay conditions used Giardia intestinalis BRIS/83/HEPU/106 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.3.2 monomer 1 * 46000, SDS-PAGE Giardia intestinalis
1.6.3.2 monomer 1 * 45116, calculated from sequence Giardia intestinalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.3.2 7.8
-
-
 Giardia intestinalis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.6.3.2 7 9 active over the range pH 7-9 Giardia intestinalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.3.2 FAD FAD is noncoveltly bound, 1 mol of FAD per mol of polypeptide Giardia intestinalis
1.6.3.2 NADH NADPH is more efficient as electron donor compared to NADH Giardia intestinalis
1.6.3.2 NADPH NADPH is more efficient as electron donor compared to NADH Giardia intestinalis

General Information

EC Number General Information Comment Organism
1.6.3.2 physiological function the enzyme may be involved in maintenance of an optimum intracellular redox ratio Giardia intestinalis