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Literature summary extracted from

  • Kengen, S.W.; Luesink, E.J.; Stams, A.J.; Zehnder, A.J.
    Purification and characterization of an extremely thermostable beta-glucosidase from the hyperthermophilic archaeon Pyrococcus furiosus (1993), Eur. J. Biochem., 213, 305-312.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.B28 D-glucose weak Pyrococcus furiosus
3.2.1.B28 SDS 0.1%, activity is completely and irreversibly blocked Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.B28 0.15
-
 4-nitrophenyl beta-D-glucopyranoside pH 5, 80°C Pyrococcus furiosus
3.2.1.B28 20
-
 cellobiose pH 5, 80°C Pyrococcus furiosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.B28 cytoplasm
-
 Pyrococcus furiosus 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.B28 additional information divalent metal ions not required Pyrococcus furiosus
3.2.1.B28 NaCl optimal concentration: 200 mM Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.B28 58000
-
 4 * 58000, SDD-PAGE Pyrococcus furiosus
3.2.1.B28 230000
-
 non-denaturing PAGE Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.B28 Pyrococcus furiosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.B28
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.B28 4-nitrophenyl beta-D-galactopyranoside + H2O hydrolysed at 34% compared to activity with 4-nitrophenyl-beta-D-glucopyranoside Pyrococcus furiosus 4-nitrophenol + beta-D-galactopyranose
-
 ?
3.2.1.B28 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Pyrococcus furiosus 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.B28 4-nitrophenyl beta-D-xylopyranoside + H2O hydrolysed at 9.2% compared to activity with 4-nitrophenyl-beta-D-glucopyranoside Pyrococcus furiosus 4-nitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.B28 cellobiose + H2O as active as 4-nitrophenyl-beta-D-glucopyranoside Pyrococcus furiosus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.B28 tetramer 4 * 58000, SDD-PAGE Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.B28 102 105
-
 Pyrococcus furiosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.B28 80 110 80°C: about 45% of maximal activity, 110°C: about 65% of maximal activity Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.B28 100
-
 half-life: 85 h Pyrococcus furiosus
3.2.1.B28 110
-
 half-life: 13 h Pyrococcus furiosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.B28 454
-
 cellobiose pH 5, 80°C Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.B28 5
-
-
 Pyrococcus furiosus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.B28 300
-
 D-glucose pH 5, 80°C Pyrococcus furiosus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.B28 Pyrococcus furiosus isoelectric focusing
-
 4.4