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Literature summary extracted from

  • Consalvi, V.; Chiaraluce, R.; Politi, L.; Vaccaro, R.; De Rosa, M.; Scandurra, R.
    Extremely thermostable glutamate dehydrogenase from the hyperthermophilic archaebacterium Pyrococcus furiosus (1991), Eur. J. Biochem., 202, 1189-1196.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.4.1.4 the purified enzyme can withstand at least three freezing (-20°C) and thawing (25°C) cycles without significant effects on activity the enzyme can be lyophilzed and stored for several months without loss of activity Pyrococcus furiosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.4 2-oxoglutarate substrate inhibition above 2.0 mM Pyrococcus furiosus
1.4.1.4 L-glutamate substrate inhibition above 15 mM Pyrococcus furiosus
1.4.1.4 additional information no effect: EDTA, o-phenanthroline, sodium azide, 8-hydroxyquinoline in the concentration raneg of 1-10 mM Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.4 0.012
-
 NADPH pH 7.6, 60°C Pyrococcus furiosus
1.4.1.4 0.02
-
 NADP+ pH 7.6, 60°C Pyrococcus furiosus
1.4.1.4 0.36
-
 NADH pH 7.6, 50°C Pyrococcus furiosus
1.4.1.4 0.6
-
 L-glutamate pH 7.6, 60°C Pyrococcus furiosus
1.4.1.4 6
-
 2-oxoglutarate pH 7.6, 60°C Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.4 48000
-
 x * 48000, SDS-PAGE Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.4 Pyrococcus furiosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.4
-
 Pyrococcus furiosus

Storage Stability

EC Number Storage Stability Organism
1.4.1.4 25°C, 20 mM potassium phosphate, pH 6.5, containing 7 mM 2-mercaptoethanol and 10% glycerol, stable for several weeks Pyrococcus furiosus
1.4.1.4 4°C, 20 mM potassium phosphate, pH 6.5, containing 7 mM 2-mercaptoethanol and 10% glycerol, stable for several weeks Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.4 2-aminopentanoate + H2O + NADP+ activity with 2-oxovalerate amd 2-aminopentanoate is 25% and 17% compared to the activity with 2-oxoglutarate and L-glutamate Pyrococcus furiosus 2-oxopentanoate + NH3 + NADPH + H+
-
 r
1.4.1.4 2-oxoglutarate + NH3 + NADH + H+ weak activity Pyrococcus furiosus L-glutamate + H2O + NAD+
-
 ?
1.4.1.4 L-glutamate + H2O + NADP+
-
 Pyrococcus furiosus 2-oxoglutarate + NH3 + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.4.1.4 ? x * 48000, SDS-PAGE Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.4 60
-
 assay at Pyrococcus furiosus
1.4.1.4 75
-
 reductive amination Pyrococcus furiosus
1.4.1.4 80
-
 oxidation of glutamate Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.4 90
-
 20 h, stable Pyrococcus furiosus
1.4.1.4 100
-
 half-life: 12 h Pyrococcus furiosus
1.4.1.4 110
-
 half-life: 25 min Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.4 7.6
-
 assay at Pyrococcus furiosus
1.4.1.4 9
-
-
 Pyrococcus furiosus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.4 NAD+ low activity Pyrococcus furiosus
1.4.1.4 NADP+
-
 Pyrococcus furiosus
1.4.1.4 NADPH
-
 Pyrococcus furiosus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.4.1.4 Pyrococcus furiosus isoelectrofocusing
-
 4.5