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Literature summary extracted from

  • Negron, L.; Patchett, M.L.; Parker, E.J.
    Expression, purification, and characterisation of dehydroquinate synthase from Pyrococcus furiosus (2011), Enzyme Res., 2011, 134893.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.4 overexpressed in Escherichia coli Rosetta DE3. The enzyme is insoluble when expressed in Escherichia coli but is partially solubilised when KCl is included in the cell lysis buffer Pyrococcus furiosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.4 EDTA inactivates the enzyme, and enzyme activity is restored by several divalent metal ions including (in order of decreasing effectiveness) Cd2+, Co2+, Zn2+, and Mn2+ Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.4 0.0037
-
 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate pH 6.8, 60°C Pyrococcus furiosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.4 Cd2+ activates Pyrococcus furiosus
4.2.3.4 Co2+ activates Pyrococcus furiosus
4.2.3.4 Mn2+ activates Pyrococcus furiosus
4.2.3.4 Zn2+ activates Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.4 37397
-
 2 * 37397, electrospray ionisation mass spectrometry Pyrococcus furiosus
4.2.3.4 72000
-
 gel filtration Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.4 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate Pyrococcus furiosus the enzyme catalyses the second step of the shikimate pathway to aromatic compounds 3-dehydroquinate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.4 Pyrococcus furiosus Q8U0A8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.4
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.4 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate
-
 Pyrococcus furiosus 3-dehydroquinate + phosphate
-
 ?
4.2.3.4 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate the enzyme catalyses the second step of the shikimate pathway to aromatic compounds Pyrococcus furiosus 3-dehydroquinate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.4 homodimer 2 * 37397, electrospray ionisation mass spectrometry Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
4.2.3.4 DHQS
-
 Pyrococcus furiosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.3.4 90
-
 active up to Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.3.4 90
-
 Tm-value Pyrococcus furiosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.4 3
-
 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate pH 6.8, 60°C Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.4 6.8
-
-
 Pyrococcus furiosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.3.4 6.3 9.5 pH 6.3: 65% of maximal activity, pH 9.5: about 65% of maximal activity Pyrococcus furiosus

General Information

EC Number General Information Comment Organism
4.2.3.4 physiological function the enzyme catalyses the second step of the shikimate pathway to aromatic compounds Pyrococcus furiosus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.3.4 811
-
 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate pH 6.8, 60°C Pyrococcus furiosus