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Literature summary extracted from

  • Okabe, M.; Tomita, K.; Ishitani, R.; Ishii, R.; Takeuchi, N.; Arisaka, F.; Nureki, O.; Yokoyama, S.
    Divergent evolutions of trinucleotide polymerization revealed by an archaeal CCA-adding enzyme structure (2003), EMBO J., 22, 5918-5927.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.72 overexpression in Escherichia coli Archaeoglobus fulgidus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.72 crystal structures of the CCA-adding enzyme, and its complexes with CTP and ATP at 2.0, 2.0 and 2.7 A resolutions, respectively Archaeoglobus fulgidus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.72 0.05
-
 CTP pH 8.5, 50°C Archaeoglobus fulgidus
2.7.7.72 0.24
-
 ATP pH 8.5, 50°C Archaeoglobus fulgidus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.72 Archaeoglobus fulgidus O28126
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.72
-
 Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.72 a tRNA with a 3' CC end + ATP
-
 Archaeoglobus fulgidus a tRNA with a 3' CCA end + diphosphate
-
 ?
2.7.7.72 a tRNA with a 3' cytidine + CTP
-
 Archaeoglobus fulgidus a tRNA with a 3' CC end + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.72 ATP(CTP):tRNA nucleotidyltransferase
-
 Archaeoglobus fulgidus
2.7.7.72 CCA-adding enzyme
-
 Archaeoglobus fulgidus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.72 50
-
 assay at Archaeoglobus fulgidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.72 8.5
-
 assay at Archaeoglobus fulgidus