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Literature summary extracted from

  • Niedzwiecka, N.; Skorkowski, E.F.
    Purification and properties of malic enzyme from herring Clupea harengus spermatozoa (2013), Comp. Biochem. Physiol. B, 164, 216-220.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.39 ATP
-
 Clarias gariepinus
1.1.1.39 ATP
-
 Clupea harengus
1.1.1.39 ATP
-
 Cyprinus carpio
1.1.1.39 ATP
-
 Salmo salar
1.1.1.39 ATP
-
 Salmo trutta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.39 0.06
-
 NADH pH 7.0, 25°C Clupea harengus
1.1.1.39 0.16
-
 (S)-malate pH 7.5, 25°C Clupea harengus
1.1.1.39 0.2
-
 NAD+ pH 7.5, 25°C Clupea harengus
1.1.1.39 4.1
-
 pyruvate pH 7.0, 25°C Clupea harengus
1.1.1.39 16.8
-
 CO2 pH 7.0, 25°C Clupea harengus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.39 Mn2+ activates Cyprinus carpio
1.1.1.39 Mn2+ activates Salmo salar
1.1.1.39 Mn2+ activates Clarias gariepinus
1.1.1.39 Mn2+ activates Salmo trutta
1.1.1.39 Mn2+ activates, Km 0.08 mM in the decarboxylation/oxidation reaction Clupea harengus
1.1.1.39 Mn2+ activates, Km is 0.08 mM Clupea harengus
1.1.1.40 Mn2+ activates Cyprinus carpio
1.1.1.40 Mn2+ activates Salmo salar
1.1.1.40 Mn2+ activates Clarias gariepinus
1.1.1.40 Mn2+ activates Clupea harengus
1.1.1.40 Mn2+ activates Salmo trutta

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.39 61000
-
 4 * 61000, SDS-PAGE Clupea harengus
1.1.1.40 64000
-
 4 * 64000, SDS-PAGE Clupea harengus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.39 (S)-malate + NAD+ Cyprinus carpio
-
 pyruvate + CO2 + NADH
-
 r
1.1.1.39 (S)-malate + NAD+ Salmo salar
-
 pyruvate + CO2 + NADH
-
 r
1.1.1.39 (S)-malate + NAD+ Clarias gariepinus
-
 pyruvate + CO2 + NADH
-
 r
1.1.1.39 (S)-malate + NAD+ Clupea harengus
-
 pyruvate + CO2 + NADH
-
 r
1.1.1.39 (S)-malate + NAD+ Salmo trutta
-
 pyruvate + CO2 + NADH
-
 r
1.1.1.40 (S)-malate + NADP+ Cyprinus carpio
-
 pyruvate + CO2 + NADPH
-
 r
1.1.1.40 (S)-malate + NADP+ Salmo salar
-
 pyruvate + CO2 + NADPH
-
 r
1.1.1.40 (S)-malate + NADP+ Clarias gariepinus
-
 pyruvate + CO2 + NADPH
-
 r
1.1.1.40 (S)-malate + NADP+ Clupea harengus
-
 pyruvate + CO2 + NADPH
-
 ?
1.1.1.40 (S)-malate + NADP+ Clupea harengus
-
 pyruvate + CO2 + NADPH
-
 r
1.1.1.40 (S)-malate + NADP+ Salmo trutta
-
 pyruvate + CO2 + NADPH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.39 Clarias gariepinus
-
-
-
1.1.1.39 Clupea harengus
-
-
-
1.1.1.39 Clupea harengus
-
 from the Baltic Sea
-
1.1.1.39 Cyprinus carpio
-
-
-
1.1.1.39 Salmo salar
-
-
-
1.1.1.39 Salmo trutta
-
-
-
1.1.1.40 Clarias gariepinus
-
-
-
1.1.1.40 Clupea harengus
-
-
-
1.1.1.40 Clupea harengus
-
 from the Baltic Sea
-
1.1.1.40 Cyprinus carpio
-
-
-
1.1.1.40 Salmo salar
-
-
-
1.1.1.40 Salmo trutta
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.39 native enzyme 84.7fold by anion exchange and affinity chromatography, and gel filtration Clupea harengus
1.1.1.39 native NAD-preferring malic enzyme 84.7fold by anion exchange and affinity chromatography followed by gel filtration Clupea harengus
1.1.1.40 native enzyme by anion exchange and affinity chromatography Clupea harengus
1.1.1.40 native NADP-specific malic enzyme by anion exchange and 2'5'-ADP affinity chromatography Clupea harengus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.39 spermatozoon high exnzyme activity Clupea harengus
-
1.1.1.39 spermatozoon two molecular forms of malic enzyme are present in herring spermatozoa: an NAD-preferring malic enzyme with very high activity and an NADP-specific malic enzyme (EC 1.1.1.40) with much lower activity (ratio about 33:1) Cyprinus carpio
-
1.1.1.39 spermatozoon two molecular forms of malic enzyme are present in herring spermatozoa: an NAD-preferring malic enzyme with very high activity and an NADP-specific malic enzyme (EC 1.1.1.40) with much lower activity (ratio about 33:1) Salmo salar
-
1.1.1.39 spermatozoon two molecular forms of malic enzyme are present in herring spermatozoa: an NAD-preferring malic enzyme with very high activity and an NADP-specific malic enzyme (EC 1.1.1.40) with much lower activity (ratio about 33:1) Clarias gariepinus
-
1.1.1.39 spermatozoon two molecular forms of malic enzyme are present in herring spermatozoa: an NAD-preferring malic enzyme with very high activity and an NADP-specific malic enzyme (EC 1.1.1.40) with much lower activity (ratio about 33:1) Clupea harengus
-
1.1.1.39 spermatozoon two molecular forms of malic enzyme are present in herring spermatozoa: an NAD-preferring malic enzyme with very high activity and an NADP-specific malic enzyme (EC 1.1.1.40) with much lower activity (ratio about 33:1) Salmo trutta
-
1.1.1.40 spermatozoon two molecular forms of malic enzyme are present in carp spermatozoa: an NAD-preferring malic enzyme with very high activity and an NADP-specific malic enzyme (EC 1.1.1.40) with much lower activity (ratio about 33:1) Cyprinus carpio
-
1.1.1.40 spermatozoon two molecular forms of malic enzyme are present in catfish spermatozoa: an NAD-preferring malic enzyme with very high activity and an NADP-specific malic enzyme (EC 1.1.1.40) with much lower activity (ratio about 33:1) Clarias gariepinus
-
1.1.1.40 spermatozoon two molecular forms of malic enzyme are present in herring spermatozoa: an NAD-preferring malic enzyme with very high activity and an NADP-specific malic enzyme (EC 1.1.1.40) with much lower activity (ratio about 33:1) Clupea harengus
-
1.1.1.40 spermatozoon two molecular forms of malic enzyme are present in salmon spermatozoa: an NAD-preferring malic enzyme with very high activity and an NADP-specific malic enzyme (EC 1.1.1.40) with much lower activity (ratio about 33:1) Salmo salar
-
1.1.1.40 spermatozoon two molecular forms of malic enzyme are present in salmon spermatozoa: an NAD-preferring malic enzyme with very high activity and an NADP-specific malic enzyme (EC 1.1.1.40) with much lower activity (ratio about 33:1) Salmo trutta
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.39 4.25
-
 purified native enzyme, pH 7.0, 25°C, carboxylation reaction Clupea harengus
1.1.1.39 4.25
-
 purified native enzyme, pH 7.0, 25°C, pyruvate carboxylation Clupea harengus
1.1.1.39 36.09
-
 purified native enzyme, pH 7.5, 25°C, decarboxylation reaction Clupea harengus
1.1.1.39 36.09
-
 purified native enzyme, pH 7.5, 25°C, malate decarboxylation Clupea harengus
1.1.1.40 0.008
-
 purified native enzyme, pH 7.5, 25°C Clarias gariepinus
1.1.1.40 0.023
-
 purified native enzyme, pH 7.5, 25°C Salmo trutta
1.1.1.40 0.026
-
 purified native enzyme, pH 7.5, 25°C Salmo salar
1.1.1.40 0.045
-
 purified native enzyme, pH 7.5, 25°C Cyprinus carpio
1.1.1.40 0.213
-
 purified native enzyme, pH 7.5, 25°C Clupea harengus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.39 (S)-malate + NAD+
-
 Cyprinus carpio pyruvate + CO2 + NADH
-
 r
1.1.1.39 (S)-malate + NAD+
-
 Salmo salar pyruvate + CO2 + NADH
-
 r
1.1.1.39 (S)-malate + NAD+
-
 Clarias gariepinus pyruvate + CO2 + NADH
-
 r
1.1.1.39 (S)-malate + NAD+
-
 Clupea harengus pyruvate + CO2 + NADH
-
 r
1.1.1.39 (S)-malate + NAD+
-
 Salmo trutta pyruvate + CO2 + NADH
-
 r
1.1.1.39 (S)-malate + NAD+ the decarboxylation reaction is preferred, overview Clupea harengus pyruvate + CO2 + NADH
-
 r
1.1.1.39 pyruvate + CO2 + NADH the rate of carboxylation of pyruvate to malate is lower than for the decarboxylation reaction Clupea harengus (S)-malate + NAD+
-
 r
1.1.1.40 (S)-malate + NADP+
-
 Cyprinus carpio pyruvate + CO2 + NADPH
-
 r
1.1.1.40 (S)-malate + NADP+
-
 Salmo salar pyruvate + CO2 + NADPH
-
 r
1.1.1.40 (S)-malate + NADP+
-
 Clarias gariepinus pyruvate + CO2 + NADPH
-
 r
1.1.1.40 (S)-malate + NADP+
-
 Clupea harengus pyruvate + CO2 + NADPH
-
 ?
1.1.1.40 (S)-malate + NADP+
-
 Clupea harengus pyruvate + CO2 + NADPH
-
 r
1.1.1.40 (S)-malate + NADP+
-
 Salmo trutta pyruvate + CO2 + NADPH
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.39 ? x * 61000, SDS-PAGE Clupea harengus
1.1.1.39 tetramer 4 * 61000, SDS-PAGE Clupea harengus
1.1.1.40 ? x * 64000, SDS-PAGE Clupea harengus
1.1.1.40 tetramer 4 * 64000, SDS-PAGE Clupea harengus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.39 NAD-preferring malic enzyme
-
 Cyprinus carpio
1.1.1.39 NAD-preferring malic enzyme
-
 Salmo salar
1.1.1.39 NAD-preferring malic enzyme
-
 Clarias gariepinus
1.1.1.39 NAD-preferring malic enzyme
-
 Clupea harengus
1.1.1.39 NAD-preferring malic enzyme
-
 Salmo trutta
1.1.1.39 NAD-preferring ME
-
 Clupea harengus
1.1.1.40 NADP-specific ME
-
 Clupea harengus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.39 25
-
 assay at Cyprinus carpio
1.1.1.39 25
-
 assay at Salmo salar
1.1.1.39 25
-
 assay at Clarias gariepinus
1.1.1.39 25
-
 assay at Clupea harengus
1.1.1.39 25
-
 assay at Salmo trutta
1.1.1.40 25
-
 assay at Cyprinus carpio
1.1.1.40 25
-
 assay at Salmo salar
1.1.1.40 25
-
 assay at Clarias gariepinus
1.1.1.40 25
-
 assay at Clupea harengus
1.1.1.40 25
-
 assay at Salmo trutta

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.39 7
-
 carboxylation reaction Clupea harengus
1.1.1.39 7
-
 pyruvate carboxylation Clupea harengus
1.1.1.39 7.5
-
 assay at Cyprinus carpio
1.1.1.39 7.5
-
 assay at Salmo salar
1.1.1.39 7.5
-
 assay at Clarias gariepinus
1.1.1.39 7.5
-
 assay at Salmo trutta
1.1.1.39 7.5
-
 decarboxylation reaction Clupea harengus
1.1.1.39 7.5
-
 malate decarboxylation Clupea harengus
1.1.1.40 7
-
 malate decarboxylation Clupea harengus
1.1.1.40 7.5
-
 assay at Cyprinus carpio
1.1.1.40 7.5
-
 assay at Salmo salar
1.1.1.40 7.5
-
 assay at Clarias gariepinus
1.1.1.40 7.5
-
 assay at Clupea harengus
1.1.1.40 7.5
-
 assay at Salmo trutta

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.39 6 8 decarboxylation reaction, activity range Clupea harengus
1.1.1.39 6 9 activity range, profile overview Clupea harengus
1.1.1.39 6 7.5 carboxylation reaction, activity range Clupea harengus
1.1.1.40 6 8 activity range, profile overview Clupea harengus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.39 NAD+
-
 Cyprinus carpio
1.1.1.39 NAD+
-
 Salmo salar
1.1.1.39 NAD+
-
 Clarias gariepinus
1.1.1.39 NAD+
-
 Clupea harengus
1.1.1.39 NAD+
-
 Salmo trutta
1.1.1.39 NAD+ dependent on Clupea harengus
1.1.1.39 NADH
-
 Cyprinus carpio
1.1.1.39 NADH
-
 Salmo salar
1.1.1.39 NADH
-
 Clarias gariepinus
1.1.1.39 NADH
-
 Clupea harengus
1.1.1.39 NADH
-
 Salmo trutta
1.1.1.40 NADP+
-
 Cyprinus carpio
1.1.1.40 NADP+
-
 Salmo salar
1.1.1.40 NADP+
-
 Clarias gariepinus
1.1.1.40 NADP+
-
 Clupea harengus
1.1.1.40 NADP+
-
 Salmo trutta
1.1.1.40 NADP+ dependent on Clupea harengus
1.1.1.40 NADPH
-
 Cyprinus carpio
1.1.1.40 NADPH
-
 Salmo salar
1.1.1.40 NADPH
-
 Clarias gariepinus
1.1.1.40 NADPH
-
 Clupea harengus
1.1.1.40 NADPH
-
 Salmo trutta

General Information

EC Number General Information Comment Organism
1.1.1.39 metabolism fish spermatozoa contain a glycolytic pathway, tricarboxylic acid cycle and oxidative phosphorylation system, all of which are key pathways contributing to ATP synthesis, involving the enzyme Cyprinus carpio
1.1.1.39 metabolism fish spermatozoa contain a glycolytic pathway, tricarboxylic acid cycle and oxidative phosphorylation system, all of which are key pathways contributing to ATP synthesis, involving the enzyme Salmo salar
1.1.1.39 metabolism fish spermatozoa contain a glycolytic pathway, tricarboxylic acid cycle and oxidative phosphorylation system, all of which are key pathways contributing to ATP synthesis, involving the enzyme Clarias gariepinus
1.1.1.39 metabolism fish spermatozoa contain a glycolytic pathway, tricarboxylic acid cycle and oxidative phosphorylation system, all of which are key pathways contributing to ATP synthesis, involving the enzyme Clupea harengus
1.1.1.39 metabolism fish spermatozoa contain a glycolytic pathway, tricarboxylic acid cycle and oxidative phosphorylation system, all of which are key pathways contributing to ATP synthesis, involving the enzyme Salmo trutta
1.1.1.40 metabolism fish spermatozoa contain a glycolytic pathway, tricarboxylic acid cycle and oxidative phosphorylation system, all of which are key pathways contributing to ATP synthesis, involving the enzyme Cyprinus carpio
1.1.1.40 metabolism fish spermatozoa contain a glycolytic pathway, tricarboxylic acid cycle and oxidative phosphorylation system, all of which are key pathways contributing to ATP synthesis, involving the enzyme Salmo salar
1.1.1.40 metabolism fish spermatozoa contain a glycolytic pathway, tricarboxylic acid cycle and oxidative phosphorylation system, all of which are key pathways contributing to ATP synthesis, involving the enzyme Clarias gariepinus
1.1.1.40 metabolism fish spermatozoa contain a glycolytic pathway, tricarboxylic acid cycle and oxidative phosphorylation system, all of which are key pathways contributing to ATP synthesis, involving the enzyme Clupea harengus
1.1.1.40 metabolism fish spermatozoa contain a glycolytic pathway, tricarboxylic acid cycle and oxidative phosphorylation system, all of which are key pathways contributing to ATP synthesis, involving the enzyme Salmo trutta