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Literature summary extracted from

  • De Pascale, G.; Griffiths, E.; Shakya, T.; Nazi, I.; Wright, G.
    Identification and characterization of new inhibitors of fungal homoserine kinase (2011), ChemBioChem, 12, 1179-1182.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.39 expressed in Escherichia coli BL21(DE3) cells Schizosaccharomyces pombe

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.39 (2Z)-2-cyano-N-(2,5-dibromophenyl)-3-hydroxybut-2-enamide
-
 Schizosaccharomyces pombe
2.7.1.39 1-(naphthalen-2-yl)prop-2-en-1-one
-
 Schizosaccharomyces pombe
2.7.1.39 2-(2-methylpropyl)-6,7,8,9-tetrahydro-4H,5H-cyclohepta[4,5]thieno[2,3-d][1,3]oxazin-4-one
-
 Schizosaccharomyces pombe
2.7.1.39 2-(3,4-dihydroxyphenyl)-3,6,7-trihydroxy-2,3-dihydro-4H-chromen-4-one
-
 Schizosaccharomyces pombe
2.7.1.39 3-methyl-1-phenyl-6-(trifluoromethyl)-1H-pyrazolo[3,4-b]pyridin-4-ol
-
 Schizosaccharomyces pombe
2.7.1.39 4-hydroxy-3-[(4-hydroxy-2-oxo-3,8a-dihydro-2H-chromen-3-yl)methyl]-2H-chromen-2-one
-
 Schizosaccharomyces pombe

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.39 0.0676
-
 ATP in 50 mM HEPES, pH 7.5, 40 mM KCl, 10 mM MgCl2, at 37°C Schizosaccharomyces pombe
2.7.1.39 0.463
-
 L-homoserine in 50 mM HEPES, pH 7.5, 40 mM KCl, 10 mM MgCl2, at 37°C Schizosaccharomyces pombe

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.39 Schizosaccharomyces pombe
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.39 Ni-NTA column chromatography, gel filtration Schizosaccharomyces pombe

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.39 ATP + L-homoserine
-
 Schizosaccharomyces pombe ADP + O-phospho-L-homoserine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.39 Thr1
-
 Schizosaccharomyces pombe

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.39 11.2
-
 L-homoserine in 50 mM HEPES, pH 7.5, 40 mM KCl, 10 mM MgCl2, at 37°C Schizosaccharomyces pombe
2.7.1.39 14.2
-
 ATP in 50 mM HEPES, pH 7.5, 40 mM KCl, 10 mM MgCl2, at 37°C Schizosaccharomyces pombe

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.39 0.00046
-
 4-hydroxy-3-[(4-hydroxy-2-oxo-3,8a-dihydro-2H-chromen-3-yl)methyl]-2H-chromen-2-one in 50 mM HEPES, pH 7.5, 40 mM KCl, 10 mM MgCl2, at 37°C Schizosaccharomyces pombe
2.7.1.39 0.0086
-
 3-methyl-1-phenyl-6-(trifluoromethyl)-1H-pyrazolo[3,4-b]pyridin-4-ol in 50 mM HEPES, pH 7.5, 40 mM KCl, 10 mM MgCl2, at 37°C Schizosaccharomyces pombe
2.7.1.39 0.012
-
 (2Z)-2-cyano-N-(2,5-dibromophenyl)-3-hydroxybut-2-enamide in 50 mM HEPES, pH 7.5, 40 mM KCl, 10 mM MgCl2, at 37°C Schizosaccharomyces pombe
2.7.1.39 0.0271
-
 2-(3,4-dihydroxyphenyl)-3,6,7-trihydroxy-2,3-dihydro-4H-chromen-4-one in 50 mM HEPES, pH 7.5, 40 mM KCl, 10 mM MgCl2, at 37°C Schizosaccharomyces pombe
2.7.1.39 0.032
-
 1-(naphthalen-2-yl)prop-2-en-1-one in 50 mM HEPES, pH 7.5, 40 mM KCl, 10 mM MgCl2, at 37°C Schizosaccharomyces pombe
2.7.1.39 0.0358
-
 2-(2-methylpropyl)-6,7,8,9-tetrahydro-4H,5H-cyclohepta[4,5]thieno[2,3-d][1,3]oxazin-4-one in 50 mM HEPES, pH 7.5, 40 mM KCl, 10 mM MgCl2, at 37°C Schizosaccharomyces pombe

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.39 24.1
-
 L-homoserine in 50 mM HEPES, pH 7.5, 40 mM KCl, 10 mM MgCl2, at 37°C Schizosaccharomyces pombe
2.7.1.39 210
-
 ATP in 50 mM HEPES, pH 7.5, 40 mM KCl, 10 mM MgCl2, at 37°C Schizosaccharomyces pombe