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Literature summary extracted from

  • Lee, S.A.; Belyaeva, O.V.; Kedishvili, N.Y.
    Evidence that proteosome inhibitors and chemical chaperones can rescue the activity of retinol dehydrogenase 12 mutant T49M (2011), Chem. Biol. Interact., 191, 55-59.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.300 recombinant expression in HEK-293 cell microsomes Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.300 I51N site-directed mutagenesis, the catalytically active I51N variant of RDH12 undergoes accelerated degradation through the ubiquitin-proteosome system, which results in reduced level of the protein in the cell. The RDH12 mutant has lost its retinaldehyde reductase activity. Inhibitors of proteosome activity, e.g. MG132, or dimethyl sulfoxide can partially restore the activity Homo sapiens
1.1.1.300 T49M site-directed mutagenesis, the catalytically active T49M variant of RDH12 undergoes accelerated degradation through the ubiquitin-proteosome system, which results in reduced level of the protein in the cell.The RDH12 mutant has lost its retinaldehyde reductase activity. Inhibitors of proteosome activity, e.g. MG132, or dimethyl sulfoxide can partially restore the activity Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.300 endoplasmic reticulum membrane
-
 Homo sapiens 5789
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.300 all-trans-retinal + NADPH + H+ Homo sapiens
-
 all-trans-retinol + NADP+
-
 r
1.1.1.300 all-trans-retinol + NADP+ Homo sapiens
-
 all-trans-retinal + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.300 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.300 recombinant enzyme from HEK-293 cell microsomes Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.300 eye
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.300 all-trans-retinal + NADPH + H+
-
 Homo sapiens all-trans-retinol + NADP+
-
 r
1.1.1.300 all-trans-retinol + NADP+
-
 Homo sapiens all-trans-retinal + NADPH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.300 RDH12
-
 Homo sapiens
1.1.1.300 retinaldehyde reductase
-
 Homo sapiens
1.1.1.300 retinol dehydrogenase 12
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.300 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.300 7.4
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.300 NADP+ dependent on Homo sapiens
1.1.1.300 NADPH
-
 Homo sapiens