Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Li, L.; Liu, Y.; Wang, W.; Cheng, J.; Zhao, W.; Wang, P.
    A highly efficient galactokinase from Bifidobacterium infantis with broad substrate specificity (2012), Carbohydr. Res., 355, 35-39.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.6 expressed in Escherichia coli Bifidobacterium longum subsp. infantis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.6 0.895
-
 D-galactose pH 8, 37°C Bifidobacterium longum subsp. infantis
2.7.1.6 2.46
-
 ATP pH 8, 37°C Bifidobacterium longum subsp. infantis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.6 Mg2+ required Bifidobacterium longum subsp. infantis
2.7.1.6 Mn2+ can partly substitute Mg2+, with the reduction of the activity by about 21% Bifidobacterium longum subsp. infantis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.6 45000
-
 SDS-PAGE Bifidobacterium longum subsp. infantis

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.6 Bifidobacterium longum subsp. infantis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.6 using Ni-NTA chromatography Bifidobacterium longum subsp. infantis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.6 0.0781
-
 substrate: ATP + 6-deoxy-D-galactose, pH 8, 37°C Bifidobacterium longum subsp. infantis
2.7.1.6 0.09
-
 substrate: ATP + D-galacturonic acid, pH 8, 37°C Bifidobacterium longum subsp. infantis
2.7.1.6 0.52
-
 substrate: UTP + D-galactose, pH 8, 37°C Bifidobacterium longum subsp. infantis
2.7.1.6 0.551
-
 substrate: CTP + D-galactose, pH 8, 37°C Bifidobacterium longum subsp. infantis
2.7.1.6 0.931
-
 substrate: dCTP + D-galactose, pH 8, 37°C Bifidobacterium longum subsp. infantis
2.7.1.6 1.5
-
 substrate: dUTP + D-galactose, pH 8, 37°C Bifidobacterium longum subsp. infantis
2.7.1.6 2.5
-
 substrate: dTTP + D-galactose, pH 8, 37°C Bifidobacterium longum subsp. infantis
2.7.1.6 12.5
-
 substrate: ATP + D-galactosamine, pH 8, 37°C Bifidobacterium longum subsp. infantis
2.7.1.6 15.7
-
 substrate: ITP + D-galactose, pH 8, 37°C Bifidobacterium longum subsp. infantis
2.7.1.6 28.5
-
 substrate: GTP + D-galactose, pH 8, 37°C Bifidobacterium longum subsp. infantis
2.7.1.6 31.9
-
 substrate: dGTP + D-galactose, pH 8, 37°C Bifidobacterium longum subsp. infantis
2.7.1.6 99.7
-
 substrate: ATP + 2-deoxy-D-galactose, pH 8, 37°C Bifidobacterium longum subsp. infantis
2.7.1.6 137
-
 substrate: ATP + D-galactose, pH 8, 37°C Bifidobacterium longum subsp. infantis
2.7.1.6 138
-
 substrate: dATP + D-galactose, pH 8, 37°C Bifidobacterium longum subsp. infantis
2.7.1.6 158
-
 substrate: ATP + D-galactose, pH 8, 37°C Bifidobacterium longum subsp. infantis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.6 ATP + 2-deoxy-D-galactose substrate: 2-deoxygalactose, specific activity: 99.7 micromol/min/mg Bifidobacterium longum subsp. infantis ADP + 2-deoxy-alpha-D-galactose 1-phosphate
-
 ?
2.7.1.6 ATP + 6-deoxy-D-galactose substrate: 6-deoxygalactose, specific activity: 0.0781 micromol/min/mg Bifidobacterium longum subsp. infantis ADP + 6-deoxy-alpha-D-galactose 1-phosphate
-
 ?
2.7.1.6 ATP + D-galactosamine substrate: D-galactosamine, specific activity: 12.5 micromol/min/mg Bifidobacterium longum subsp. infantis ADP + alpha-D-galactosamine 1-phosphate
-
 ?
2.7.1.6 ATP + D-galactose substrate: ATP, specific activity: 137 micromol/min/mg Bifidobacterium longum subsp. infantis ADP + alpha-D-galactose 1-phosphate
-
 ?
2.7.1.6 ATP + D-galactose substrate: D-galactose, specific activity: 158 micromol/min/mg Bifidobacterium longum subsp. infantis ADP + alpha-D-galactose 1-phosphate
-
 ?
2.7.1.6 ATP + D-galacturonic acid substrate: galacturonic acid, specific activity: 0.09 micromol/min/mg Bifidobacterium longum subsp. infantis ADP + alpha-D-galacturonic acid 1-phosphate
-
 ?
2.7.1.6 CTP + D-galactose substrate: CTP, specific activity: 0.551 micromol/min/mg Bifidobacterium longum subsp. infantis CDP + alpha-D-galactose 1-phosphate
-
 ?
2.7.1.6 dATP + D-galactose substrate: dATP, specific activity: 138 micromol/min/mg Bifidobacterium longum subsp. infantis dADP + alpha-D-galactose 1-phosphate
-
 ?
2.7.1.6 dCTP + D-galactose substrate: dCTP, specific activity: 0.931 micromol/min/mg Bifidobacterium longum subsp. infantis dCDP + alpha-D-galactose 1-phosphate
-
 ?
2.7.1.6 dGTP + D-galactose substrate: dGTP, specific activity: 31.9 micromol/min/mg Bifidobacterium longum subsp. infantis dGDP + alpha-D-galactose 1-phosphate
-
 ?
2.7.1.6 dTTP + D-galactose substrate: dTTP, specific activity: 2.5 micromol/min/mg Bifidobacterium longum subsp. infantis ?
-
 ?
2.7.1.6 dUTP + D-galactose substrate: dUTP, specific activity: 1.5 micromol/min/mg Bifidobacterium longum subsp. infantis dUDP + alpha-D-galactose 1-phosphate
-
 ?
2.7.1.6 GTP + D-galactose substrate: GTP, specific activity: 28.5 micromol/min/mg Bifidobacterium longum subsp. infantis GDP + alpha-D-galactose 1-phosphate
-
 ?
2.7.1.6 ITP + D-galactose substrate: ITP, specific activity: 15.7 micromol/min/mg Bifidobacterium longum subsp. infantis IDP + alpha-D-galactose 1-phosphate
-
 ?
2.7.1.6 UTP + D-galactose substrate: UTP, specific activity: 0.52 micromol/min/mg Bifidobacterium longum subsp. infantis UDP + alpha-D-galactose 1-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.6 BiGalK
-
 Bifidobacterium longum subsp. infantis
2.7.1.6 galactokinase
-
 Bifidobacterium longum subsp. infantis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.6 37
-
 assay at Bifidobacterium longum subsp. infantis
2.7.1.6 55
-
 highest activity Bifidobacterium longum subsp. infantis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.6 4 30 only 20% of maximal activity Bifidobacterium longum subsp. infantis
2.7.1.6 65
-
 over 76% of maximal activity Bifidobacterium longum subsp. infantis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.6 146
-
 ATP pH 8, 37°C Bifidobacterium longum subsp. infantis
2.7.1.6 146
-
 D-galactose pH 8, 37°C Bifidobacterium longum subsp. infantis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.6 8
-
 assay at Bifidobacterium longum subsp. infantis
2.7.1.6 8.5
-
 optimal pH Bifidobacterium longum subsp. infantis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.6 7 9 enzyme keeps over 60% of maximal activity Bifidobacterium longum subsp. infantis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.6 59.5
-
 ATP pH 8, 37°C Bifidobacterium longum subsp. infantis
2.7.1.6 164
-
 D-galactose pH 8, 37°C Bifidobacterium longum subsp. infantis