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Literature summary extracted from

  • Lin, M.; Ye, S.; Xiong, Y.; Cai, D.; Zhang, J.; Hu, Y.
    Expression and characterization of RNA-dependent RNA polymerase of Ectropis obliqua virus (2010), BMB Rep., 43, 284-290.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.48
-
 Ectropis obliqua picorna-like virus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.48 Mg2+ and Mn2+, both required. Maximum activity in presence of 5.0 mM Mg2+ and 1.0-2.0 mM Mn2+ Ectropis obliqua picorna-like virus
2.7.7.48 Mn2+ and Mg2+, both required. Maximum activity in presence of 5.0 mM Mg2+ and 1.0-2.0 mM Mn2+ Ectropis obliqua picorna-like virus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.48 66000
-
 x * 66000, calculated, x * 68000, SDS-PAGE Ectropis obliqua picorna-like virus
2.7.7.48 68000
-
 x * 66000, calculated, x * 68000, SDS-PAGE Ectropis obliqua picorna-like virus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.48 Ectropis obliqua picorna-like virus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.48 nucleoside triphosphate + RNAn enzyme initiates RNA synthesis in a primer- and poly(A)-dependent manner in vitro Ectropis obliqua picorna-like virus diphosphate + RNAn+1 product is double-stranded RNA ?

Subunits

EC Number Subunits Comment Organism
2.7.7.48 ? x * 66000, calculated, x * 68000, SDS-PAGE Ectropis obliqua picorna-like virus

Synonyms

EC Number Synonyms Comment Organism
2.7.7.48 RDRP
-
 Ectropis obliqua picorna-like virus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.48 30
-
-
 Ectropis obliqua picorna-like virus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.7.48 35
-
 sharp decrease in activity above Ectropis obliqua picorna-like virus