EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.28 | expression of the His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Pediococcus acidilactici |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.28 | Cu2+ | inhibits 35% at 1 mM | Pediococcus acidilactici | |
1.1.1.28 | Fe2+ | inhibits 25% at 1 mM | Pediococcus acidilactici |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.28 | 0.09 | - |
pyruvate | recombinant enzyme, pH 5.5, 30°C | Pediococcus acidilactici | |
1.1.1.28 | 2.9 | - |
phenylpyruvate | recombinant enzyme, pH 5.5, 30°C | Pediococcus acidilactici |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.28 | Mn2+ | activates by 25% at 1 mM | Pediococcus acidilactici | |
1.1.1.28 | additional information | poor effects by 1 mM Ca2+, Zn2+, Mg2+, Ba2+, and Co2+ | Pediococcus acidilactici |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.28 | 37000 | - |
x * 37000, recombinant His6-tagged enzyme, SDS-PAGE | Pediococcus acidilactici |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.28 | (R)-lactate + NAD+ | Pediococcus acidilactici | - |
pyruvate + NADH + H+ | - |
r | |
1.1.1.28 | (R)-lactate + NAD+ | Pediococcus acidilactici DSM 20284 | - |
pyruvate + NADH + H+ | - |
r | |
1.1.1.28 | phenylpyruvate + NADH + H+ | Pediococcus acidilactici | - |
(R)-3-phenyllactate + NAD+ | - |
? | |
1.1.1.28 | phenylpyruvate + NADH + H+ | Pediococcus acidilactici DSM 20284 | - |
(R)-3-phenyllactate + NAD+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.28 | Pediococcus acidilactici | - |
- |
- |
1.1.1.28 | Pediococcus acidilactici DSM 20284 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.28 | recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Pediococcus acidilactici |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.28 | 140 | - |
purified recombinant enzyme, substrate phenylpyruvate, pH 5.5, 30°C | Pediococcus acidilactici |
1.1.1.28 | 422 | - |
purified recombinant enzyme, substrate pyruvate, pH 5.5, 30°C | Pediococcus acidilactici |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.28 | (R)-lactate + NAD+ | - |
Pediococcus acidilactici | pyruvate + NADH + H+ | - |
r | |
1.1.1.28 | (R)-lactate + NAD+ | - |
Pediococcus acidilactici DSM 20284 | pyruvate + NADH + H+ | - |
r | |
1.1.1.28 | phenylpyruvate + NADH + H+ | - |
Pediococcus acidilactici | (R)-3-phenyllactate + NAD+ | - |
? | |
1.1.1.28 | phenylpyruvate + NADH + H+ | - |
Pediococcus acidilactici DSM 20284 | (R)-3-phenyllactate + NAD+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.28 | ? | x * 37000, recombinant His6-tagged enzyme, SDS-PAGE | Pediococcus acidilactici |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.28 | D-LDH | - |
Pediococcus acidilactici |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.28 | 30 | - |
- |
Pediococcus acidilactici |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.28 | 20 | 70 | activity range, profile, overview | Pediococcus acidilactici |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.28 | 30 | 40 | the enzyme is significantly stable below 30°C, but the activity decreases significantly above 40°C | Pediococcus acidilactici |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.28 | 305 | - |
phenylpyruvate | recombinant enzyme, pH 5.5, 30°C | Pediococcus acidilactici |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.28 | 5.5 | - |
- |
Pediococcus acidilactici |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.28 | 4 | 7 | activity range, profile, overview | Pediococcus acidilactici |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.28 | NAD+ | - |
Pediococcus acidilactici | |
1.1.1.28 | NADH | - |
Pediococcus acidilactici |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.28 | 105 | - |
phenylpyruvate | recombinant enzyme, pH 5.5, 30°C | Pediococcus acidilactici | |
1.1.1.28 | 3157 | - |
pyruvate | recombinant enzyme, pH 5.5, 30°C | Pediococcus acidilactici |