Literature summary extracted from

Lang, M.; Kamrat, T.; Nidetzky, B.
Influence of ionic liquid cosolvent on transgalactosylation reactions catalyzed by thermostable beta-glycosylhydrolase CelB from Pyrococcus furiosus (2006), Biotechnol. Bioeng., 95, 1093-1100.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
3.2.1.B28	1,3-dimethylimidazolium methylsulfate	the enzyme retains full catalytic efficiency for lactose hydrolysis at 80°C in a 50% (v/v) solution of the ionic liquid 1,3-dimethylimidazoliummethylsulfate in sodium citrate buffer, pH 5.5. It is inactive but not irreversibly denatured at 70% (v/v) ionic liquid	Pyrococcus furiosus

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.2.1.B28	Pyrococcus furiosus	Q51723	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.2.1.B28	additional information	the transferase activity of the enzyme can be improved significantly by addition of the ionic liquid 1,3-dimethylimidazoliummethylsulfate. The relative enhancement of the selectivity of the enzyme for glycosyl transfer to the acceptor nucleophile compared with water by the cosolvent is strongly dependent on the structure of the acceptor, suggesting that interactions of the acceptor with solvent molecules, water or ionic liquid, make a major contribution to the observed transgalactosylation specificity of the enzyme	Pyrococcus furiosus	?	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
3.2.1.B28	CelB	-	Pyrococcus furiosus

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Release 2023.1 (January 2023)